PHF10 (NM_018288) Human Tagged ORF Clone

CAT#: RC210609

PHF10 (Myc-DDK-tagged)-Human PHD finger protein 10 (PHF10), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY413136) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210609 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHF10
• Synonyms: BAF45A; XAP135
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210609 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCAAGAACAAGTCAGTGAATATTTGGGTGTGACCTCCTTTAAAAGGAAATATCCAGATTTAGAGC
  GACGAGATTTGTCTCACAAGGAGAAACTCTACCTGAGAGAGCTAAATGTCATTACTGAAACTCAGTGCAC
  TCTAGGCTTAACAGCATTGCGCAGTGATGAAGTGATTGATTTAATGATAAAAGAATATCCAGCCAAACAT
  GCTGAGTATTCTGTTATTCTACAAGAAAAAGAACGTCAACGAATTACAGACCATTATAAAGAGTATTCCC
  AAATGCAACAACAGAATACTCAGAAAGTTGAAGCCAGTAAAGTGCCTGAGTATATTAAGAAAGCTGCCAA
  AAAAGCAGCAGAATTTAATAGCAACTTAAACCGGGAACGCATGGAAGAAAGAAGAGCTTATTTTGACTTG
  CAGACACATGTTATCCAGGTACCTCAAGGGAAGTACAAAGTTTTGCCAACAGAGCGAACAAAGGTCAGTT
  CTTACCCAGTGGCTCTCATCCCCGGACAGTTCCAGGAATATTATAAGAGGTACTCACCAGATGAGCTGCG
  GTATCTGCCATTAAACACAGCCCTGTATGAGCCCCCTCTGGATCCTGAGCTCCCTGCTCTAGACAGTGAT
  GGTGATTCAGATGATGGCGAAGATGGTCGAGGTGATGAGAAACGGAAAAATAAAGGCACTTCGGACAGCT
  CCTCTGGCAATGTATCTGAAGGGGAAAGCCCTCCTGACAGCCAGGAGGACTCTTTCCAGGGAAGACAGAA
  ATCAAAAGACAAAGCTGCCACTCCAAGAAAAGATGGTCCCAAACGTTCTGTACTGTCCAAGTCAGTTCCT
  GGGTACAAGCCAAAGGTCATTCCAAATGCTATATGTGGAATTTGTCTGAAGGGTAAGGAGTCCAACAAGA
  AAGGAAAGGCTGAATCACTTATACACTGCTCCCAATGTGAGAATAGTGGCCATCCTTCTTGCCTGGATAT
  GACAATGGAGCTTGTTTCTATGATTAAGACCTACCCATGGCAGTGTATGGAATGTAAAACATGCATTATA
  TGTGGACAACCCCACCATGAAGAAGAAATGATGTTCTGTGATATGTGTGACAGAGGTTATCATACTTTTT
  GTGTGGGCCTTGGTGCTATTCCATCAGGTCGCTGGATTTGTGACTGTTGTCAGCGGGCCCCCCCAACACC
  CAGGAAAGTGGGCAGAAGGGGGAAAAACAGCAAAGAGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210609 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLQEQVSEYLGVTSFKRKYPDLERRDLSHKEKLYLRELNVITETQCTLGLTALRSDEVIDLMIKEYPAKH
  AEYSVILQEKERQRITDHYKEYSQMQQQNTQKVEASKVPEYIKKAAKKAAEFNSNLNRERMEERRAYFDL
  QTHVIQVPQGKYKVLPTERTKVSSYPVALIPGQFQEYYKRYSPDELRYLPLNTALYEPPLDPELPALDSD
  GDSDDGEDGRGDEKRKNKGTSDSSSGNVSEGESPPDSQEDSFQGRQKSKDKAATPRKDGPKRSVLSKSVP
  GYKPKVIPNAICGICLKGKESNKKGKAESLIHCSQCENSGHPSCLDMTMELVSMIKTYPWQCMECKTCII
  CGQPHHEEEMMFCDMCDRGYHTFCVGLGAIPSGRWICDCCQRAPPTPRKVGRRGKNSKEG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018288
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018288.1, NM_018288.2, NP_060758.1
• RefSeq Size: 1692 bp
• RefSeq ORF: 1497 bp
• Locus ID: 55274
• Domains: PHD
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• MW: 46.6 kDa
• Gene Summary: This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28. [provided by RefSeq, Jul 2008]