Lactate Dehydrogenase C (LDHC) (NM_017448) Human Tagged ORF Clone
CAT#: RC210627
LDHC (Myc-DDK-tagged)-Human lactate dehydrogenase C (LDHC), transcript variant 2
"NM_017448" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | LDHC |
Synonyms | CT32; LDH3; LDHX |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210627 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAACTGTCAAGGAGCAGCTAATTGAGAAGCTAATTGAGGATGATGAAAACTCCCAGTGTAAAATTA CTATTGTTGGAACTGGTGCCGTAGGCATGGCTTGTGCTATTAGTATCTTACTGAAGGATTTGGCTGATGA ACTTGCCCTTGTTGATGTTGCATTGGACAAACTGAAGGGAGAAATGATGGATCTTCAGCATGGCAGTCTT TTCTTTAGTACTTCAAAGATTACTTCTGGAAAAGATTACAGTGTATCTGCAAACTCCAGAATAGTTATTG TCACAGCAGGTGCAAGGCAGCAGGAGGGAGAAACTCGCCTTGCCCTGGTCCAACGTAATGTGGCTATAAT GAAATCAATCATTCCTGCCATAGTCCATTATAGTCCTGATTGTAAAATTCTTGTTGTTTCAAATCCAGTG GATATTTTGACATATATAGTCTGGAAGATAAGTGGCTTACCTGTAACTCGTGTAATTGGAAGTGGTTGTA ATCTAGACTCTGCCCGTTTCCGTTACCTAATTGGAGAAAAGTTGGGTGTCCACCCCACAAGCTGCCATGG TTGGATTATTGGAGAACATGGTGATTCTAGTGTGCCCTTATGGAGTGGGGTGAATGTTGCTGGTGTTGCT CTGAAGACTCTGGACCCTAAATTAGGAACGGATTCAGATAAGGAACACTGGAAAAATATCCATAAACAAG TTATTCAAAGTGCCTATGAAATTATCAAGCTGAAGGGGTATACCTCTTGGGCTATTGGACTGTCTGTGAT GGATCTGGTAGGATCCATTTTGAAAAATCTTAGGAGAGTGCACCCAGTTTCCACCATGGTTAAGGGATTA TATGGAATAAAAGAAGAACTCTTTCTCAGTATCCCTTGTGTCTTGGGGCGGAATGGTGTCTCAGATGTTG TGAAAATTAACTTGAATTCTGAGGAGGAGGCCCTTTTCAAGAAGAGTGCAGAAACACTTTGGAATATTCA AAAGGATCTAATATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210627 protein sequence
Red=Cloning site Green=Tags(s) MSTVKEQLIEKLIEDDENSQCKITIVGTGAVGMACAISILLKDLADELALVDVALDKLKGEMMDLQHGSL FFSTSKITSGKDYSVSANSRIVIVTAGARQQEGETRLALVQRNVAIMKSIIPAIVHYSPDCKILVVSNPV DILTYIVWKISGLPVTRVIGSGCNLDSARFRYLIGEKLGVHPTSCHGWIIGEHGDSSVPLWSGVNVAGVA LKTLDPKLGTDSDKEHWKNIHKQVIQSAYEIIKLKGYTSWAIGLSVMDLVGSILKNLRRVHPVSTMVKGL YGIKEELFLSIPCVLGRNGVSDVVKINLNSEEEALFKKSAETLWNIQKDLIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017448 |
ORF Size | 996 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017448.1, NM_017448.2, NM_017448.3, NP_059144.1 |
RefSeq Size | 1264 bp |
RefSeq ORF | 999 bp |
Locus ID | 3948 |
Cytogenetics | 11p15.1 |
Protein Pathways | Cysteine and methionine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism |
MW | 36.3 kDa |
Gene Summary | ' Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC304466 | LDHC (untagged)-Human lactate dehydrogenase C (LDHC), transcript variant 2 |
USD 660.00 |
|
SC322033 | LDHC (untagged)-Human lactate dehydrogenase C (LDHC), transcript variant 2 |
USD 660.00 |
|
RG210627 | LDHC (GFP-tagged) - Human lactate dehydrogenase C (LDHC), transcript variant 2 |
USD 460.00 |
|
RC210627L3 | Lenti ORF clone of Human lactate dehydrogenase C (LDHC), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC210627L4 | Lenti ORF clone of Human lactate dehydrogenase C (LDHC), transcript variant 2, mGFP tagged |
USD 620.00 |
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