Metabotropic Glutamate Receptor 8 (GRM8) (NM_000845) Human Tagged ORF Clone

CAT#: RC210813

GRM8 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 8 (GRM8), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY426746) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226256 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRM8
• Synonyms: GLUR8; GPRC1H; mGlu8; MGLUR8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210813 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTATGCGAGGGAAAGCGATCAGCCTCTTGCCCTTGTTTCTTCCTCTTGACCGCCAAGTTCTACTGGA
  TCCTCACAATGATGCAAAGAACTCACAGCCAGGAGTATGCCCATTCCATACGGGTGGATGGGGACATTAT
  TTTGGGGGGTCTCTTCCCTGTCCACGCAAAGGGAGAGAGAGGGGTGCCTTGTGGGGAGCTGAAGAAGGAA
  AAGGGGATTCACAGACTGGAGGCCATGCTTTATGCAATTGACCAGATTAACAAGGACCCTGATCTCCTTT
  CCAACATCACTCTGGGTGTCCGCATCCTCGACACGTGCTCTAGGGACACCTATGCTTTGGAGCAGTCTCT
  AACATTCGTGCAGGCATTAATAGAGAAAGATGCTTCGGATGTGAAGTGTGCTAATGGAGATCCACCCATT
  TTCACCAAGCCCGACAAGATTTCTGGCGTCATAGGTGCTGCAGCAAGCTCCGTGTCCATCATGGTTGCTA
  ACATTTTAAGACTTTTTAAGATACCTCAAATCAGCTATGCATCCACAGCCCCAGAGCTAAGTGATAACAC
  CAGGTATGACTTTTTCTCTCGAGTGGTTCCGCCTGACTCCTACCAAGCCCAAGCCATGGTGGACATCGTG
  ACAGCACTGGGATGGAATTATGTTTCGACACTGGCTTCTGAGGGGAACTATGGTGAGAGCGGTGTGGAGG
  CCTTCACCCAGATCTCGAGGGAGATTGGTGGTGTTTGCATTGCTCAGTCACAGAAAATCCCACGTGAACC
  AAGACCTGGAGAATTTGAAAAAATTATCAAACGCCTGCTAGAAACACCTAATGCTCGAGCAGTGATTATG
  TTTGCCAATGAGGATGACATCAGGAGGATATTGGAAGCAGCAAAAAAACTAAACCAAAGTGGGCATTTTC
  TCTGGATTGGCTCAGATAGTTGGGGATCCAAAATAGCACCTGTCTATCAGCAAGAGGAGATTGCAGAAGG
  GGCTGTGACAATTTTGCCCAAACGAGCATCAATTGATGGATTTGATCGATACTTTAGAAGCCGAACTCTT
  GCCAATAATCGAAGAAATGTGTGGTTTGCAGAATTCTGGGAGGAGAATTTTGGCTGCAAGTTAGGATCAC
  ATGGGAAAAGGAACAGTCATATAAAGAAATGCACAGGGCTGGAGCGAATTGCTCGGGATTCATCTTATGA
  ACAGGAAGGAAAGGTCCAATTTGTAATTGATGCTGTATATTCCATGGCTTACGCCCTGCACAATATGCAC
  AAAGATCTCTGCCCTGGATACATTGGCCTTTGTCCACGAATGAGTACCATTGATGGGAAAGAGCTACTTG
  GTTATATTCGGGCTGTAAATTTTAATGGCAGTGCTGGCACTCCTGTCACTTTTAATGAAAACGGAGATGC
  TCCTGGACGTTATGATATCTTCCAGTATCAAATAACCAACAAAAGCACAGAGTACAAAGTCATCGGCCAC
  TGGACCAATCAGCTTCATCTAAAAGTGGAAGACATGCAGTGGGCTCATAGAGAACATACTCACCCGGCGT
  CTGTCTGCAGCCTGCCGTGTAAGCCAGGGGAGAGGAAGAAAACGGTGAAAGGGGTCCCTTGCTGCTGGCA
  CTGTGAACGCTGTGAAGGTTACAACTACCAGGTGGATGAGCTGTCCTGTGAACTTTGCCCTCTGGATCAG
  AGACCCAACATGAACCGCACAGGCTGCCAGCTTATCCCCATCATCAAATTGGAGTGGCATTCTCCCTGGG
  CTGTGGTGCCTGTGTTTGTTGCAATATTGGGAATCATCGCCACCACCTTTGTGATCGTGACCTTTGTCCG
  CTATAATGACACACCTATCGTGAGGGCTTCAGGACGCGAACTTAGTTACGTGCTCCTAACGGGGATTTTT
  CTCTGTTATTCAATCACGTTTTTAATGATTGCAGCACCAGATACAATCATATGCTCCTTCCGACGGGTCT
  TCCTAGGACTTGGCATGTGTTTCAGCTATGCAGCCCTTCTGACCAAAACAAACCGTATCCACCGAATATT
  TGAGCAGGGGAAGAAATCTGTCACAGCGCCCAAGTTCATTAGTCCAGCATCTCAGCTGGTGATCACCTTC
  AGCCTCATCTCCGTCCAGCTCCTTGGAGTGTTTGTCTGGTTTGTTGTGGATCCCCCCCACATCATCATTG
  ACTATGGAGAGCAGCGGACACTAGATCCAGAGAAGGCCAGGGGAGTGCTCAAGTGTGACATTTCTGATCT
  CTCACTCATTTGTTCACTTGGATACAGTATCCTCTTGATGGTCACTTGTACTGTTTATGCCATTAAAACG
  AGAGGTGTCCCAGAGACTTTCAATGAAGCCAAACCTATTGGATTTACCATGTATACCACCTGCATCATTT
  GGTTAGCTTTCATCCCCATCTTTTTTGGTACAGCCCAGTCAGCAGAAAAGATGTACATCCAGACAACAAC
  ACTTACTGTCTCCATGAGTTTAAGTGCTTCAGTATCTCTGGGCATGCTCTATATGCCCAAGGTTTATATT
  ATAATTTTTCATCCAGAACAGAATGTTCAAAAACGCAAGAGGAGCTTCAAGGCTGTGGTGACAGCTGCCA
  CCATGCAAAGCAAACTGATCCAAAAAGGAAATGACAGACCAAATGGCGAGGTGAAAAGTGAACTCTGTGA
  GAGTCTTGAAACCAACACTTCCTCTACCAAGACAACATATATCAGTTACAGCAATCATTCAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210813 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVCEGKRSASCPCFFLLTAKFYWILTMMQRTHSQEYAHSIRVDGDIILGGLFPVHAKGERGVPCGELKKE
  KGIHRLEAMLYAIDQINKDPDLLSNITLGVRILDTCSRDTYALEQSLTFVQALIEKDASDVKCANGDPPI
  FTKPDKISGVIGAAASSVSIMVANILRLFKIPQISYASTAPELSDNTRYDFFSRVVPPDSYQAQAMVDIV
  TALGWNYVSTLASEGNYGESGVEAFTQISREIGGVCIAQSQKIPREPRPGEFEKIIKRLLETPNARAVIM
  FANEDDIRRILEAAKKLNQSGHFLWIGSDSWGSKIAPVYQQEEIAEGAVTILPKRASIDGFDRYFRSRTL
  ANNRRNVWFAEFWEENFGCKLGSHGKRNSHIKKCTGLERIARDSSYEQEGKVQFVIDAVYSMAYALHNMH
  KDLCPGYIGLCPRMSTIDGKELLGYIRAVNFNGSAGTPVTFNENGDAPGRYDIFQYQITNKSTEYKVIGH
  WTNQLHLKVEDMQWAHREHTHPASVCSLPCKPGERKKTVKGVPCCWHCERCEGYNYQVDELSCELCPLDQ
  RPNMNRTGCQLIPIIKLEWHSPWAVVPVFVAILGIIATTFVIVTFVRYNDTPIVRASGRELSYVLLTGIF
  LCYSITFLMIAAPDTIICSFRRVFLGLGMCFSYAALLTKTNRIHRIFEQGKKSVTAPKFISPASQLVITF
  SLISVQLLGVFVWFVVDPPHIIIDYGEQRTLDPEKARGVLKCDISDLSLICSLGYSILLMVTCTVYAIKT
  RGVPETFNEAKPIGFTMYTTCIIWLAFIPIFFGTAQSAEKMYIQTTTLTVSMSLSASVSLGMLYMPKVYI
  IIFHPEQNVQKRKRSFKAVVTAATMQSKLIQKGNDRPNGEVKSELCESLETNTSSTKTTYISYSNHSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000845
• ORF Size: 2724 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000845.1, NM_000845.2, NP_000836.1
• RefSeq Size: 3572 bp
• RefSeq ORF: 2727 bp
• Locus ID: 2918
• Cytogenetics: 7q31.33
• Domains: 7tm_3, ANF_receptor
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 101.7 kDa
• Gene Summary: 'L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'