RCE1 (NM_001032279) Human Tagged ORF Clone

CAT#: RC210814

RCE1 (Myc-DDK-tagged)-Human RCE1 homolog, prenyl protein peptidase (S. cerevisiae) (RCE1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY422291) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210814 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RCE1
• Synonyms: FACE2; RCE1A; RCE1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210814 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCTGGGCGGGGATGGGCTGCGACTGCTGTCGGTGTCGCGGCCGGAGCGGCCGCCCGAGTCGG
  CGGCGCTGGGCGGCCTGGGCCCCGGGCTGTGCTGCTGGGTGTCAGTGTTCTCCTGCCTCAGCCTCGCCTG
  CTCCTACGTGGGCAGCCTCTACGTCTGGAAGAGCGAACTGCCCAGGGACCATCCCGCGGTCATCAAGCGA
  CGCTTCACCAGCGTCCTGGTGGTGTCCAGTCTCTCACCCCTGTGCGTGCTGCTCTGGAGGGAACTCACAG
  GCATCCAGCCAGGCACATCCCTGCTCACCCTGATGGGCTTCAGGCTGGAGGGCATTTTCCCAGCGGCGCT
  GCTGCCCCTGTTGCTGACCATGATTCTTTTCCTGGGCCCACTGATGCAGCTCTCTATGGATTGCCCTTGT
  GACCTGGCAGATGGGCTGAAGGTTGTCCTGGCCCCCCGCTCCTGGGCCCGCTGCCTCACAGACATGCGTT
  GGCTGCGGAACCAAGTGATCGCCCCGCTGACAGAGGAGCTGGTGTTCCGGGCCTGTATGCTGCCCATGTT
  AGCACCGTGCATGGGCCTGGGCCCTGCTGTGTTCACCTGCCCGCTCTTTTTTGGAGTTGCCCATTTTCAC
  CATATTATTGAGCAGCTGCGTTTCCGCCAGAGCAGCGTGGGGAACATCTTCTTGTCTGCTGCGTTCCAGT
  TCTCCTACACAGCTGTCTTCGGTGCCTACACTGCTTTCCTCTTCATCCGCACAGGACACCTGATTGGGCC
  GGTTCTCTGCCATTCCTTCTGCAATTACATGGGTTTCCCAGCTGTTTGCGCGGCCTTGGAGCACCCACAG
  AGGCGGCCCCTGCTGGCAGGCTATGCCCTGGGTGTGGGACTCTTCCTGCTTCTGCTCCAGCCCCTCACGG
  ACCCCAAGCTCTACGGCAGCCTTCCCCTTTGTGTGCTTTTGGAGCGGGCAGGGGACTCAGAGGCTCCCCT
  GTGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210814 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAALGGDGLRLLSVSRPERPPESAALGGLGPGLCCWVSVFSCLSLACSYVGSLYVWKSELPRDHPAVIKR
  RFTSVLVVSSLSPLCVLLWRELTGIQPGTSLLTLMGFRLEGIFPAALLPLLLTMILFLGPLMQLSMDCPC
  DLADGLKVVLAPRSWARCLTDMRWLRNQVIAPLTEELVFRACMLPMLAPCMGLGPAVFTCPLFFGVAHFH
  HIIEQLRFRQSSVGNIFLSAAFQFSYTAVFGAYTAFLFIRTGHLIGPVLCHSFCNYMGFPAVCAALEHPQ
  RRPLLAGYALGVGLFLLLLQPLTDPKLYGSLPLCVLLERAGDSEAPLCS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001032279
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001032279.1, NP_001027450.1
• RefSeq Size: 1485
• RefSeq ORF: 678
• Locus ID: 9986
• Protein Families: Protease, Transmembrane
• MW: 35.8 kDa
• Gene Summary: This gene encodes an integral membrane protein which is classified as a member of the metalloproteinase family. This enzyme is thought to function in the maintenance and processing of CAAX-type prenylated proteins. [provided by RefSeq, Jul 2008]