EGF (NM_001963) Human Tagged ORF Clone
CAT#: RC210817L2
- LentiORF®
Lenti ORF clone of Human epidermal growth factor (EGF), transcript variant 1, mGFP tagged
"NM_001963" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | EGF |
Synonyms | HOMG4; URG |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC210817).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001963 |
ORF Size | 3621 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001963.2, NP_001954.1 |
RefSeq Size | 5600 bp |
RefSeq ORF | 3624 bp |
Locus ID | 1950 |
Cytogenetics | 4q25 |
Domains | ldl_recept_b, EGF_CA, EGF, EGF |
Protein Families | Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane |
Protein Pathways | Bladder cancer, Cytokine-cytokine receptor interaction, Endocytosis, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton |
MW | 133.9 kDa |
Gene Summary | 'This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC127840 | EGF (untagged)-Human epidermal growth factor (EGF), transcript variant 1 |
USD 310.00 |
|
RC210817 | EGF (Myc-DDK-tagged)-Human epidermal growth factor (EGF), transcript variant 1 |
USD 1,270.00 |
|
RG210817 | EGF (GFP-tagged) - Human epidermal growth factor (EGF), transcript variant 1 |
USD 1,400.00 |
|
RC210817L1 | Lenti ORF clone of Human epidermal growth factor (EGF), transcript variant 1, Myc-DDK-tagged |
USD 1,824.00 |
|
RC210817L3 | Lenti ORF clone of Human epidermal growth factor (EGF), transcript variant 1, Myc-DDK-tagged |
USD 1,470.00 |
|
RC210817L4 | Lenti ORF clone of Human epidermal growth factor (EGF), transcript variant 1, mGFP tagged |
USD 1,470.00 |
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