HS3ST2 (NM_006043) Human Tagged ORF Clone

CAT#: RC210819

HS3ST2 (Myc-DDK-tagged)-Human heparan sulfate (glucosamine) 3-O-sulfotransferase 2 (HS3ST2)

Product Images

Western blot validation of overexpression lysate (Cat# LY416916) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210819 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HS3ST2
• Synonyms: 3OST2; 30ST2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210819 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTATAGGGTCCTGGGCCGCGCGGGGCCACCTCAGCCGCGGAGGGCGCGCAGGCTGCTCTTCGCCT
  TCACGCTCTCGCTCTCCTGCACTTACCTGTGTTACAGCTTCCTGTGCTGCTGCGACGACCTGGGTCGGAG
  CCGCCTCCTCGGCGCGCCTCGCTGCCTCCGCGGCCCCAGCGCGGGCGGCCAGAAACTTCTCCAGAAGTCC
  CGCCCCTGTGATCCCTCCGGGCCGACGCCCAGCGAGCCCAGCGCTCCCAGCGCGCCCGCCGCCGCCGTGC
  CCGCCCCTCGCCTCTCCGGTTCCAACCACTCCGGCTCACCCAAGCTGGGTACCAAGCGGTTGCCCCAAGC
  CCTCATTGTGGGCGTGAAGAAGGGGGGCACCCGGGCCGTGCTGGAGTTTATCCGAGTACACCCGGACGTG
  CGGGCCTTGGGCACGGAACCCCACTTCTTTGACAGGAACTACGGCCGCGGGCTGGATTGGTACAGGAGCC
  TGATGCCCAGGACCCTCGAGAGCCAGATCACGCTGGAGAAGACGCCCAGCTACTTTGTCACTCAAGAGGC
  TCCTCGACGCATCTTCAACATGTCCCGAGACACCAAGCTGATCGTGGTTGTGCGGAACCCTGTGACCCGT
  GCCATCTCTGATTACACGCAGACACTCTCCAAGAAGCCCGACATCCCGACCTTTGAGGGCCTCTCCTTCC
  GCAACCGCACCCTGGGCCTGGTGGACGTGTCATGGAACGCCATCCGCATCGGCATGTACGTGCTGCACCT
  GGAGAGCTGGCTGCAGTACTTCCCGCTAGCTCAGATTCACTTCGTCAGTGGCGAGCGACTCATCACTGAC
  CCGGCCGGCGAGATGGGGCGAGTCCAGGACTTCCTGGGCATTAAGAGATTCATCACGGACAAGCACTTCT
  ATTTCAACAAGACCAAAGGATTCCCTTGCTTGAAAAAAACAGAATCGAGCCTCCTGCCTCGATGCTTGGG
  CAAATCAAAAGGGAGAACTCATGTACAGATTGATCCTGAAGTGATAGACCAGCTCCGAGAATTTTATAGA
  CCGTATAATATCAAATTTTATGAAACCGTTGGGCAGGACTTCAGGTGGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210819 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAYRVLGRAGPPQPRRARRLLFAFTLSLSCTYLCYSFLCCCDDLGRSRLLGAPRCLRGPSAGGQKLLQKS
  RPCDPSGPTPSEPSAPSAPAAAVPAPRLSGSNHSGSPKLGTKRLPQALIVGVKKGGTRAVLEFIRVHPDV
  RALGTEPHFFDRNYGRGLDWYRSLMPRTLESQITLEKTPSYFVTQEAPRRIFNMSRDTKLIVVVRNPVTR
  AISDYTQTLSKKPDIPTFEGLSFRNRTLGLVDVSWNAIRIGMYVLHLESWLQYFPLAQIHFVSGERLITD
  PAGEMGRVQDFLGIKRFITDKHFYFNKTKGFPCLKKTESSLLPRCLGKSKGRTHVQIDPEVIDQLREFYR
  PYNIKFYETVGQDFRWE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006043
• ORF Size: 1101 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006043.1, NP_006034.1
• RefSeq Size: 1968 bp
• RefSeq ORF: 1104 bp
• Locus ID: 9956
• Domains: Sulfotransfer
• Protein Families: Transmembrane
• Protein Pathways: Heparan sulfate biosynthesis
• MW: 41.5 kDa
• Gene Summary: Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It is a type II integral membrane protein and possesses heparan sulfate glucosaminyl 3-O-sulfotransferase activity. This gene is expressed predominantly in brain and may play a role in the nervous system. [provided by RefSeq, Jul 2008]