ABTB2 (NM_145804) Human Tagged ORF Clone

CAT#: RC210896

ABTB2 (Myc-DDK-tagged)-Human ankyrin repeat and BTB (POZ) domain containing 2 (ABTB2)

Product Images

Western blot validation of overexpression lysate (Cat# LY407856) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210896 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABTB2
• Synonyms: ABTB2A; BTBD22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210896 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCCGGCGACGGGCTGCGCCGGGGCAAGTCCGCGCGCTGCGGCCTCACCTTCTCAGTGGGTCGCT
  TTTTCCGCTGGATGGTGGACACCCGAATCTCCGTGCGCATCCACGAGTACGCAGCCATCTCCCTCACCGC
  CTGCATGGAGAACCTGGTGGAGGAGATCCGGGCCAGGGTGATGGCCAGCCACAGCCCTGATGGCGGAGGG
  GCCGGAGGCGGGGAGGTGTCTGCTGAGGCCCTGGAGATGGTCATCAACAACGACGCCGAGCTCTGGGGCG
  TCTTGCAGCCCTATGAGCATCTCATCTGCGGCAAGAACGCCAATGGTGTCCTCTCCCTCCCCGCATACTT
  CAGCCCCTACAACGGCGGGTCCCTGGGCCATGACGAGCGAGCCGATGCCTATGCCCAGCTGGAGCTCCGA
  ACCCTGGAGCAGTCCCTCCTGGCCACCTGCGTGGGCAGCATCTCGGAGCTGAGTGACTTGGTCTCCCGTG
  CCATGCACCACATGCAGGGGCGTCACCCCCTGTGCCCGGGTGCCAGCCCTGCCCGCCAGGCCCGCCAGCC
  GCCACAGCCCATCACTTGGTCCCCCGACGCCCTCCACACGCTCTACTACTTTCTGCGGTGTCCACAGATG
  GAGTCCATGGAGAACCCCAACCTGGACCCCCCGAGAATGACCTTGAACAATGAACGGCCCTTCATGCTGC
  TGCCGCCCCTCATGGAGTGGATGCGCGTGGCCATCACCTACGCAGAGCACCGCCGCAGCCTCACCGTGGA
  CAGCGGCGACATCCGGCAGGCAGCCCGGCTGCTGCTGCCTGGTCTGGACTGTGAACCTCGGCAGCTCAAA
  CCCGAACACTGTTTCAGTTCCTTCCGGAGGCTGGATGCCCGAGCAGCTACTGAAAAATTCAACCAGGACC
  TGGGTTTCCGCATGCTCAACTGTGGGAGGACGGACCTCATCAACCAAGCCATCGAGGCCTTGGGTCCGGA
  TGGGGTTAACACCATGGATGACCAGGGTATGACGCCACTGATGTACGCCTGCGCTGCTGGGGACGAAGCG
  ATGGTCCAGATGTTGATTGATGCTGGGGCAAACTTGGACATCCAGGTTCCAAGCAACTCCCCCAGGCACC
  CTTCCATCCACCCCGACAGCCGGCACTGGACCTCACTGACATTCGCTGTGCTGCATGGACACATCTCTGT
  GGTCCAGTTGCTGCTGGATGCTGGTGCCCATGTCGAGGGCTCGGCAGTGAACGGCGGCGAGGACAGCTAT
  GCGGAGACGCCCCTGCAGCTGGCCTCTGCGGCAGGGAACTATGAGCTGGTCAGTTTGTTGCTGAGCCGAG
  GCGCCGACCCCCTCCTCAGCATGCTGGAGGCCCACGGCATGGGCTCCTCCCTCCACGAGGACATGAACTG
  CTTCAGCCACTCAGCTGCCCACGGCCACAGGAACGTCCTGAGGAAGCTCCTGACGCAGCCACAGCAGGCT
  AAAGCGGACGTGCTGTCCCTGGAGGAGATCCTGGCCGAGGGTGTGGAGGAAAGTGATGCGTCGAGCCAGG
  GCAGTGGCAGCGAGGGGCCCGTGCGGCTGAGCCGGACCCGCACCAAGGCCCTACAGGAGGCCATGTACTA
  CAGCGCTGAGCACGGCTACGTGGACATCACCATGGAGCTGAGGGCACTGGGAGTCCCCTGGAAGCTGCAC
  ATCTGGATCGAGTCTCTGAGGACCTCGTTCTCGCAGTCGCGGTACTCGGTGGTGCAGAGCCTCCTGCGGG
  ACTTCAGCTCCATCAGAGAGGAGGAGTACAACGAGGAGCTGGTGACCGAGGGCTTGCAGCTCATGTTCGA
  CATCCTCAAGACCAGCAAAAACGACTCCGTCATCCAGCAACTGGCTACCATCTTCACCCACTGCTATGGC
  AGCAGTCCCATCCCCAGCATCCCAGAGATCCGGAAGACCCTGCCGGCCAGGCTAGATCCACACTTTTTGA
  ACAATAAGGAGATGTCAGATGTGACCTTCCTGGTGGAAGGAAAGCTGTTTTATGCACATAAAGTCCTGCT
  GGTGACAGCTTCTAACAGGTTCAAGACACTAATGACCAATAAATCAGAACAGGATGGGGACAGCAGCAAG
  ACCATCGAGATCAGCGACATGAAGTACCACATTTTTCAGATGATGATGCAGTATCTGTACTACGGAGGAA
  CAGAATCCATGGAGATCCCCACCACTGACATCCTGGAGCTGCTGTCAGCTGCCAGCCTGTTCCAGCTGGA
  TGCCCTGCAGAGGCACTGCGAGATCCTGTGCTCCCAGACCCTCAGCATGGAGAGTGCCGTGAACACCTAC
  AAATATGCCAAGATCCACAATGCCCCAGAACTGGCCCTGTTCTGTGAGGGCTTCTTCCTCAAGCACATGA
  AGGCCCTACTGGAGCAGGATGCCTTCCGGCAGCTCATCTACGGCCGCAGCAGCAAAGTGCAGGGCCTGGA
  TCCACTGCAGGACCTGCAGAACACCCTGGCAGAGCGCGTGCACTCTGTCTACATCACCTCCCGGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210896 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSAGDGLRRGKSARCGLTFSVGRFFRWMVDTRISVRIHEYAAISLTACMENLVEEIRARVMASHSPDGGG
  AGGGEVSAEALEMVINNDAELWGVLQPYEHLICGKNANGVLSLPAYFSPYNGGSLGHDERADAYAQLELR
  TLEQSLLATCVGSISELSDLVSRAMHHMQGRHPLCPGASPARQARQPPQPITWSPDALHTLYYFLRCPQM
  ESMENPNLDPPRMTLNNERPFMLLPPLMEWMRVAITYAEHRRSLTVDSGDIRQAARLLLPGLDCEPRQLK
  PEHCFSSFRRLDARAATEKFNQDLGFRMLNCGRTDLINQAIEALGPDGVNTMDDQGMTPLMYACAAGDEA
  MVQMLIDAGANLDIQVPSNSPRHPSIHPDSRHWTSLTFAVLHGHISVVQLLLDAGAHVEGSAVNGGEDSY
  AETPLQLASAAGNYELVSLLLSRGADPLLSMLEAHGMGSSLHEDMNCFSHSAAHGHRNVLRKLLTQPQQA
  KADVLSLEEILAEGVEESDASSQGSGSEGPVRLSRTRTKALQEAMYYSAEHGYVDITMELRALGVPWKLH
  IWIESLRTSFSQSRYSVVQSLLRDFSSIREEEYNEELVTEGLQLMFDILKTSKNDSVIQQLATIFTHCYG
  SSPIPSIPEIRKTLPARLDPHFLNNKEMSDVTFLVEGKLFYAHKVLLVTASNRFKTLMTNKSEQDGDSSK
  TIEISDMKYHIFQMMMQYLYYGGTESMEIPTTDILELLSAASLFQLDALQRHCEILCSQTLSMESAVNTY
  KYAKIHNAPELALFCEGFFLKHMKALLEQDAFRQLIYGRSSKVQGLDPLQDLQNTLAERVHSVYITSRV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145804
• ORF Size: 2517 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145804.1, NP_665803.1
• RefSeq Size: 4919 bp
• RefSeq ORF: 3078 bp
• Locus ID: 25841
• MW: 93.3 kDa