Pyrin (MEFV) (NM_000243) Human Tagged ORF Clone

CAT#: RC210934

MEFV (Myc-DDK-tagged)-Human Mediterranean fever (MEFV), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY424850) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210934 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MEFV
• Synonyms: FMF; MEF; PAAND; TRIM20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210934 representing NM_000243
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAAGACCCCTAGTGACCATCTGCTGTCCACCCTGGAGGAGCTGGTGCCCTATGACTTCGAGAAGT
  TCAAGTTCAAGCTGCAGAACACCAGTGTGCAGAAGGAGCACTCCAGGATCCCCCGGAGCCAGATCCAGAG
  AGCCAGGCCGGTGAAGATGGCCACTCTGCTGGTCACCTACTATGGGGAAGAGTACGCCGTGCAGCTCACC
  CTGCAGGTCCTGCGGGCCATCAACCAGCGCCTGCTGGCCGAGGAGCTCCACAGGGCAGCCATTCAGGAAT
  ATTCCACACAAGAAAACGGCACAGACGATTCCGCAGCGTCCAGCTCCCTGGGGGAGAACAAGCCCAGGAG
  CCTGAAGACTCCAGACCACCCCGAGGGGAACGAGGGGAACGGCCCTCGGCCGTACGGGGGCGGGGCTGCC
  AGCCTGCGGTGCAGCCAGCCCGAGGCCGGGAGGGGGCTGTCGAGGAAGCCCCTGAGCAAACGCAGAGAGA
  AGGCATCGGAGGGCCTGGACGCGCAGGGCAAGCCTCGGACCCGGAGCCCGGCCCTGCCGGGCGGGAGAAG
  CCCCGGCCCCTGCAGGGCGCTAGAGGGGGGCCAGGCCGAGGTCCAGCTGCGCAGAAACGCCAGCTCCGCG
  GGGAGGCTGCAGGGGCTGGCGGGGGGCGCCCCGGGGCAGAAGGAGTGCAGGCCCTTCGAAGTGTACCTGC
  CCTCGGGAAAGATGCGACCTAGAAGCCTTGAGGTCACCATTTCTACAGGGGAGAAGGCGCCCGCAAATCC
  AGAAATTCTCCTGACTCTAGAGGAAAAGACAGCTGCGAATCTGGACTCGGCAACAGAACCCCGGGCAAGG
  CCCACTCCGGATGGAGGGGCATCTGCGGACCTGAAGGAAGGCCCTGGAAATCCAGAACATTCGGTCACCG
  GAAGGCCACCAGACACGGCTGCGAGTCCCCGTTGCCACGCCCAGGAAGGAGACCCAGTTGACGGTACCTG
  TGTGCGTGATTCCTGCAGCTTCCCCGAGGCAGTTTCTGGGCACCCCCAGGCCTCAGGCAGCCGCTCACCT
  GGCTGCCCCCGGTGCCAGGACTCCCATGAAAGGAAGAGCCCGGGAAGCCTAAGCCCCCAGCCCCTGCCAC
  AGTGTAAGCGCCACCTGAAGCAGGTCCAGCTGCTCTTCTGTGAGGATCACGATGAGCCCATCTGCCTCAT
  CTGCAGTCTGAGTCAGGAGCACCAAGGCCACCGGGTGCGCCCCATTGAGGAGGTCGCCCTGGAACACAAG
  AAGAAAATTCAGAAGCAGCTGGAGCATCTGAAGAAGCTGAGAAAATCAGGGGAGGAGCAGCGATCCTATG
  GGGAGGAGAAGGCAGTGAGCTTTCTGAAACAAACTGAAGCGCTGAAGCAGCGGGTGCAGAGGAAGCTGGA
  GCAGGTGTACTACTTCCTGGAACAGCAGGAGCATTTCTTTGTGGCCTCACTGGAGGACGTGGGCCAGATG
  GTTGGGCAGATCAGGAAGGCATATGACACCCGCGTATCCCAGGACATCGCCCTGCTCGACGCGCTGATTG
  GGGAACTGGAGGCCAAGGAGTGCCAGTCAGAATGGGAACTTCTGCAGGACATTGGAGACATCTTGCACAG
  GGCTAAGACAGTGCCTGTCCCTGAAAAGTGGACCACTCCTCAAGAGATAAAACAAAAGATCCAACTCCTC
  CACCAGAAGTCAGAGTTTGTGGAGAAGAGCACAAAGTACTTCTCAGAAACCCTGCGTTCAGAAATGGAAA
  TGTTCAATGTTCCGGAGCTGATTGGCGCTCAGGCACATGCTGTTAATGTGATTCTGGATGCAGAAACCGC
  TTACCCCAACCTCATCTTCTCTGATGATCTGAAGAGTGTTAGACTTGGAAACAAGTGGGAGAGGCTGCCT
  GATGGCCCGCAAAGATTTGACAGCTGTATCATTGTTCTGGGCTCTCCGAGTTTCCTCTCTGGCCGCCGTT
  ACTGGGAGGTGGAGGTTGGAGACAAGACAGCATGGATCCTGGGAGCCTGCAAGACATCCATAAGCAGGAA
  AGGGAACATGACTCTGTCGCCAGAGAATGGCTACTGGGTGGTGATAATGATGAAGGAAAATGAGTACCAG
  GCGTCCAGCGTTCCCCCGACCCGCCTGCTAATAAAGGAGCCTCCCAAGCGTGTGGGCATCTTCGTGGACT
  ACAGAGTTGGAAGCATCTCCTTTTACAATGTGACAGCCAGATCCCACATCTATACATTCGCCAGCTGCTC
  TTTCTCTGGGCCCCTTCAACCTATCTTCAGCCCTGGGACACGTGATGGAGGGAAGAACACAGCTCCTCTG
  ACTATCTGTCCAGTGGGTGGTCAGGGGCCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210934 representing NM_000243
  Red=Cloning site Green=Tags(s)
  
  MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLT
  LQVLRAINQRLLAEELHRAAIQEYSTQENGTDDSAASSSLGENKPRSLKTPDHPEGNEGNGPRPYGGGAA
  SLRCSQPEAGRGLSRKPLSKRREKASEGLDAQGKPRTRSPALPGGRSPGPCRALEGGQAEVQLRRNASSA
  GRLQGLAGGAPGQKECRPFEVYLPSGKMRPRSLEVTISTGEKAPANPEILLTLEEKTAANLDSATEPRAR
  PTPDGGASADLKEGPGNPEHSVTGRPPDTAASPRCHAQEGDPVDGTCVRDSCSFPEAVSGHPQASGSRSP
  GCPRCQDSHERKSPGSLSPQPLPQCKRHLKQVQLLFCEDHDEPICLICSLSQEHQGHRVRPIEEVALEHK
  KKIQKQLEHLKKLRKSGEEQRSYGEEKAVSFLKQTEALKQRVQRKLEQVYYFLEQQEHFFVASLEDVGQM
  VGQIRKAYDTRVSQDIALLDALIGELEAKECQSEWELLQDIGDILHRAKTVPVPEKWTTPQEIKQKIQLL
  HQKSEFVEKSTKYFSETLRSEMEMFNVPELIGAQAHAVNVILDAETAYPNLIFSDDLKSVRLGNKWERLP
  DGPQRFDSCIIVLGSPSFLSGRRYWEVEVGDKTAWILGACKTSISRKGNMTLSPENGYWVVIMMKENEYQ
  ASSVPPTRLLIKEPPKRVGIFVDYRVGSISFYNVTARSHIYTFASCSFSGPLQPIFSPGTRDGGKNTAPL
  TICPVGGQGPD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000243
• ORF Size: 2343 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000243.1, NM_000243.2, NP_000234.1
• RefSeq Size: 3511 bp
• RefSeq ORF: 2346 bp
• Locus ID: 4210
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• MW: 86.3 kDa
• Gene Summary: 'This gene encodes a protein, also known as pyrin or marenostrin, that is an important modulator of innate immunity. Mutations in this gene are associated with Mediterranean fever, a hereditary periodic fever syndrome. [provided by RefSeq, Jul 2008]'