ADCK5 (NM_174922) Human Tagged ORF Clone

CAT#: RC210973

ADCK5 (Myc-DDK-tagged)-Human aarF domain containing kinase 5 (ADCK5)

Product Images

Western blot validation of overexpression lysate (Cat# LY406404) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210973 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADCK5
• Synonyms: FLJ35454; MGC126708
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210973 representing NM_174922
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCGACCGGTGCAGCTCTGTCATTTCCACTCTGCTCTGCTGCACAGCAGGCAGAAGCCCTGGCCGT
  CCCCTGCTGTGTTCTTCAGGAGAAACGTCAGGGGCCTTCCTCCAAGGTTCTCCAGCCCCACACCCCTGTG
  GAGGAAGGTGCTCTCCACCGCGGTAGTGGGGGCGCCCCTGCTCCTCGGAGCCCGCTATGTCATGGCAGAG
  GCACGGGAGAAGAGGAGGATGCGGCTCGTGGTGGATGGCATGGGGCGCTTTGGCAGGTCTCTGAAGGTCG
  GCCTGCAGATCTCCCTGGACTACTGGTGGTGCACCAATGTTGTCCTTCGAGGGGTGGAAGAGAACAGCCC
  AGGCTACTTGGAGGTGATGTCTGCGTGTCACCAGCGGGCGGCTGATGCCCTGGTGGCAGGGGCCATCAGC
  AACGGGGGCCTCTACGTGAAGCTGGGCCAGGGGCTGTGCTCCTTCAACCACCTGCTTCCCCCCGAGTATA
  CCCGGACCCTGCGCGTGCTAGAGGACAGGGCCCTCAAGCGGGGCTTCCAGGAGGTGGATGAGTTGTTCCT
  TGAGGACTTCCAGGCCCTCCCCCACGAGCTCTTCCAGGAGTTTGACTACCAGCCAATTGCTGCCGCCAGC
  CTGGCACAGGTGCACAGAGCCAAGCTGCACGATGGCACCAGCGTGGCTGTGAAGGTGCAGTACATCGACC
  TGCGGGACCGCTTTGATGGGGACATCCACACCCTGGAGCTCCTGCTGCGGCTCGTTGAGGTCATGCACCC
  CAGCTTTGGCTTCAGCTGGGTCCTCCAGGACCTGAAGGGGACCCTGGCCCAGGAGCTGGACTTCGAGAAT
  GAGGGCCGCAACGCAGAGCGCTGTGCGCGGGAGCTGGCGCACTTCCCCTACGTCGTGGTGCCCCGCGTGC
  ACTGGGACAAGTCCAGCAAGCGCGTGCTCACTGCCGACTTCTGCGCCGGCTGCAAGGTCAACGATGTGGA
  GGCCATCAGGAGCCAGGGGCTGGCAGTGCATGACATAGCAGAAAAGCTCATCAAGGCCTTTGCTGAGCAG
  ATATTTTACACCGGCTTCATCCACTCGGACCCACATCCTGGCAACGTTCTGGTGCGGAAAGGCCCGGACG
  GGAAAGCGGAGCTGGTGCTGCTGGACCACGGGCTCTACCAGTTCCTGGAGGAGAAGGACCGCGCAGCCCT
  CTGCCAGCTGTGGCGGGCCATCATCCTGCGGGACGACGCCGCCATGAGGGCGCACGCAGCCGCACTGGGG
  GTGCAAGACTACCTCCTGTTCGCCGAGATGCTCATGCAGCGCCCCGTGCGCCTGGGGCAGCTGTGGGGCT
  CGCACCTACTGAGCCGCGAAGAGGCGGCCTACATGGTGGACATGGCCCGCGAGCGCTTCGAGGCCGTCAT
  GGCGGTGCTCAGGGAGCTGCCGCGGCCCATGCTGCTGGTGCTGCGCAACATCAACACCGTGCGCGCTATC
  AACGTGGCCCTCGGCGCCCCCGTGGACCGCTACTTCCTTATGGCTAAAAGGGCTGTCCGGGGCTGGAGCC
  GCCTGGCGGGCGCCACGTATCGGGGTGTCTACGGCACCAGCCTCCTGCGCCACGCCAAGGTCGTCTGGGA
  GATGCTCAAGTTTGAAGTGGCGCTCAGGCTGGAGACCTTGGCCATGCGGCTGACCGCCCTCCTGGCTCGT
  GCTCTGGTCCACCTGAGCCTCGTGCCCCCAGCGGAGGAGCTCTACCAGTACCTGGAGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210973 representing NM_174922
  Red=Cloning site Green=Tags(s)
  
  MWRPVQLCHFHSALLHSRQKPWPSPAVFFRRNVRGLPPRFSSPTPLWRKVLSTAVVGAPLLLGARYVMAE
  AREKRRMRLVVDGMGRFGRSLKVGLQISLDYWWCTNVVLRGVEENSPGYLEVMSACHQRAADALVAGAIS
  NGGLYVKLGQGLCSFNHLLPPEYTRTLRVLEDRALKRGFQEVDELFLEDFQALPHELFQEFDYQPIAAAS
  LAQVHRAKLHDGTSVAVKVQYIDLRDRFDGDIHTLELLLRLVEVMHPSFGFSWVLQDLKGTLAQELDFEN
  EGRNAERCARELAHFPYVVVPRVHWDKSSKRVLTADFCAGCKVNDVEAIRSQGLAVHDIAEKLIKAFAEQ
  IFYTGFIHSDPHPGNVLVRKGPDGKAELVLLDHGLYQFLEEKDRAALCQLWRAIILRDDAAMRAHAAALG
  VQDYLLFAEMLMQRPVRLGQLWGSHLLSREEAAYMVDMARERFEAVMAVLRELPRPMLLVLRNINTVRAI
  NVALGAPVDRYFLMAKRAVRGWSRLAGATYRGVYGTSLLRHAKVVWEMLKFEVALRLETLAMRLTALLAR
  ALVHLSLVPPAEELYQYLET
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_174922
• ORF Size: 1740 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174922.2, NM_174922.3, NM_174922.4, NP_777582.3
• RefSeq Size: 1969 bp
• RefSeq ORF: 1743 bp
• Locus ID: 203054
• Protein Families: Druggable Genome, Protein Kinase
• MW: 65.7 kDa
• Gene Summary: The function of this protein is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr). [UniProtKB/Swiss-Prot Function]