ARA9 (AIP) (NM_003977) Human Tagged ORF Clone

CAT#: RC211157

AIP (Myc-DDK-tagged)-Human aryl hydrocarbon receptor interacting protein (AIP)

Product Images

Western blot validation of overexpression lysate (Cat# LY401308) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211157 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AIP protein (Cat# TP311157). The protein was produced from HEK293T cells transfected with AIP cDNA clone (Cat# RC211157) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AIP
• Synonyms: ARA9; FKBP16; FKBP37; PITA1; SMTPHN; XAP-2; XAP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211157 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATATCATCGCAAGACTCCGGGAGGACGGGATCCAAAAACGTGTGATACAGGAAGGCCGAGGAG
  AGCTCCCGGACTTTCAAGATGGGACCAAGGCCACGTTCCACTACCGGACGCTGCACAGTGACGACGAGGG
  CACCGTGCTGGACGACAGCCGGGCTCGTGGCAAGCCCATGGAGCTCATCATTGGCAAGAAGTTCAAGCTG
  CCTGTGTGGGAGACCATCGTGTGCACCATGCGAGAAGGGGAGATTGCCCAGTTCCTCTGTGACATCAAGC
  ATGTGGTCCTGTACCCGCTGGTGGCCAAGAGTCTCCGCAACATCGCGGTGGGCAAGGACCCCCTGGAGGG
  CCAGCGGCACTGCTGCGGTGTTGCACAGATGCGTGAACACAGCTCCCTGGGCCATGCTGACCTGGACGCC
  CTGCAGCAGAACCCCCAGCCCCTCATCTTCCACATGGAGATGCTGAAGGTGGAGAGCCCTGGCACGTACC
  AGCAGGACCCATGGGCCATGACAGACGAAGAGAAGGCAAAGGCAGTGCCACTTATCCACCAGGAGGGCAA
  CCGGTTGTACCGCGAGGGGCATGTGAAGGAGGCTGCTGCCAAGTACTACGATGCCATTGCCTGCCTCAAG
  AACCTGCAGATGAAGGAACAGCCTGGGTCCCCTGAATGGATCCAGCTGGACAAGCAGATCACGCCGCTGC
  TGCTCAACTACTGCCAGTGCAAGCTGGTGGTCGAGGAGTACTACGAGGTGCTGGACCACTGCTCTTCCAT
  CCTCAACAAGTACGACGACAACGTCAAGGCCTACTTCAAGCGGGGCAAGGCCCACGCGGCCGTGTGGAAT
  GCCCAGGAGGCCCAGGCTGACTTTGCCAAAGTGCTGGAGCTGGACCCAGCCCTGGCGCCTGTGGTGAGCC
  GAGAGCTGCGGGCCCTGGAGGCACGGATCCGGCAGAAGGACGAAGAGGACAAAGCCCGGTTCCGGGGGAT
  CTTCTCCCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211157 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADIIARLREDGIQKRVIQEGRGELPDFQDGTKATFHYRTLHSDDEGTVLDDSRARGKPMELIIGKKFKL
  PVWETIVCTMREGEIAQFLCDIKHVVLYPLVAKSLRNIAVGKDPLEGQRHCCGVAQMREHSSLGHADLDA
  LQQNPQPLIFHMEMLKVESPGTYQQDPWAMTDEEKAKAVPLIHQEGNRLYREGHVKEAAAKYYDAIACLK
  NLQMKEQPGSPEWIQLDKQITPLLLNYCQCKLVVEEYYEVLDHCSSILNKYDDNVKAYFKRGKAHAAVWN
  AQEAQADFAKVLELDPALAPVVSRELRALEARIRQKDEEDKARFRGIFSH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003977
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003977.1, NM_003977.2, NM_003977.3, NP_003968.1
• RefSeq Size: 1250 bp
• RefSeq ORF: 993 bp
• Locus ID: 9049
• Protein Families: Druggable Genome, Transcription Factors
• MW: 37.7 kDa
• Gene Summary: The protein encoded by this gene is a receptor for aryl hydrocarbons and a ligand-activated transcription factor. The encoded protein is found in the cytoplasm as part of a multiprotein complex, but upon binding of ligand is transported to the nucleus. This protein can regulate the expression of many xenobiotic metabolizing enzymes. Also, the encoded protein can bind specifically to and inhibit the activity of hepatitis B virus. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]