Interferon alpha 8 (IFNA8) (NM_002170) Human Tagged ORF Clone

CAT#: RC211169

IFNA8 (Myc-DDK-tagged)-Human interferon, alpha 8 (IFNA8)

Product Images

Western blot validation of overexpression lysate (Cat# LY419487) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211169 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified IFNA8 protein (Cat# TP311169). The protein was produced from HEK293T cells transfected with IFNA8 cDNA clone (Cat# RC211169) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IFNA8
• Synonyms: IFN-alphaB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211169 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTGACTTTTTATTTACTGGTGGCCCTAGTGGTGCTCAGCTACAAGTCATTCAGCTCTCTGGGCT
  GTGATCTGCCTCAGACTCACAGCCTGGGTAACAGGAGGGCCTTGATACTCCTGGCACAAATGCGAAGAAT
  CTCTCCTTTCTCCTGCCTGAAGGACAGACATGACTTTGAATTCCCCCAGGAGGAGTTTGATGATAAACAG
  TTCCAGAAGGCTCAAGCCATCTCTGTCCTCCATGAGATGATCCAGCAGACCTTCAACCTCTTCAGCACAA
  AGGACTCATCTGCTGCTTTGGATGAGACCCTTCTAGATGAATTCTACATCGAACTTGACCAGCAGCTGAA
  TGACCTGGAGTCCTGTGTGATGCAGGAAGTGGGGGTGATAGAGTCTCCCCTGATGTACGAGGACTCCATC
  CTGGCTGTGAGGAAATACTTCCAAAGAATCACTCTATATCTGACAGAGAAGAAATACAGCTCTTGTGCCT
  GGGAGGTTGTCAGAGCAGAAATCATGAGATCCTTCTCTTTATCAATCAACTTGCAAAAAAGATTGAAGAG
  TAAGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211169 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALTFYLLVALVVLSYKSFSSLGCDLPQTHSLGNRRALILLAQMRRISPFSCLKDRHDFEFPQEEFDDKQ
  FQKAQAISVLHEMIQQTFNLFSTKDSSAALDETLLDEFYIELDQQLNDLESCVMQEVGVIESPLMYEDSI
  LAVRKYFQRITLYLTEKKYSSCAWEVVRAEIMRSFSLSINLQKRLKSKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002170
• ORF Size: 567 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002170.1, NM_002170.2, NM_002170.3, NP_002161.2
• RefSeq Size: 1039 bp
• RefSeq ORF: 570 bp
• Locus ID: 3445
• Cytogenetics: 9p21.3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Antigen processing and presentation, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, Regulation of autophagy, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway
• MW: 22 kDa
• Gene Summary: ''