GPR148 (NM_207364) Human Tagged ORF Clone

CAT#: RC211242

GPR148 (Myc-DDK-tagged)-Human G protein-coupled receptor 148 (GPR148)

Product Images

Western blot validation of overexpression lysate (Cat# LY403985) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211242 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR148
• Synonyms: BTR; PGR6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211242 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGATGAGCTGGCACCTTGCCCTGTGGGCACTACAGCTTGGCCGGCCCTGATCCAGCTCATCAGCA
  AGACACCCTGCATGCCCCAAGCAGCCAGCAACACTTCCTTGGGCCTGGGGGACCTCAGGGTGCCCAGCTC
  CATGCTGTACTGGCTTTTCCTTCCCTCAAGCCTGCTGGCTGCAGCCACACTGGCTGTCAGCCCCCTGCTG
  CTGGTGACCATCCTGCGGAACCAACGGCTGCGACAGGAGCCCCACTACCTGCTCCCGGCTAACATCCTGC
  TCTCAGACCTGGCCTACATTCTCCTCCACATGCTCATCTCCTCCAGCAGCCTGGGTGGCTGGGAGCTGGG
  CCGCATGGCCTGTGGCATTCTCACTGATGCTGTCTTCGCCGCCTGCACCAGCACCATCCTGTCCTTCACC
  GCCATTGTGCTGCACACCTACCTGGCAGTCATCCATCCACTGCGCTACCTCTCCTTCATGTCCCATGGGG
  CTGCCTGGAAGGCAGTGGCCCTCATCTGGCTGGTGGCCTGCTGCTTCCCCACATTCCTTATTTGGCTCAG
  CAAGTGGCAGGATGCCCAGCTGGAGGAGCAAGGAGCTTCATACATCCTACCACCAAGCATGGGCACCCAG
  CCGGGATGTGGCCTCCTGGTCATTGTTACCTACACCTCCATTCTGTGCGTTCTGTTCCTCTGCACAGCTC
  TCATTGCCAACTGTTTCTGGAGGATCTATGCAGAGGCCAAGACTTCAGGCATCTGGGGGCAGGGCTATTC
  CCGGGCCAGGGGCACCCTGCTGATCCACTCAGTGCTGATCACATTGTACGTGAGCACAGGGGTGGTGTTC
  TCCCTGGACATGGTGCTGACCAGGTACCACCACATTGACTCTGGGACTCACACATGGCTCCTGGCAGCTA
  ACAGTGAGGTACTCATGATGCTTCCCCGTGCCATGCTCCCATACCTGTACCTGCTCCGCTACCGGCAGCT
  GTTGGGCATGGTCCGGGGCCACCTCCCATCCAGGAGGCACCAGGCCATCTTTACCATTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211242 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDELAPCPVGTTAWPALIQLISKTPCMPQAASNTSLGLGDLRVPSSMLYWLFLPSSLLAAATLAVSPLL
  LVTILRNQRLRQEPHYLLPANILLSDLAYILLHMLISSSSLGGWELGRMACGILTDAVFAACTSTILSFT
  AIVLHTYLAVIHPLRYLSFMSHGAAWKAVALIWLVACCFPTFLIWLSKWQDAQLEEQGASYILPPSMGTQ
  PGCGLLVIVTYTSILCVLFLCTALIANCFWRIYAEAKTSGIWGQGYSRARGTLLIHSVLITLYVSTGVVF
  SLDMVLTRYHHIDSGTHTWLLAANSEVLMMLPRAMLPYLYLLRYRQLLGMVRGHLPSRRHQAIFTIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207364
• ORF Size: 1041 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_207364.1, NM_207364.2, NP_997247.1
• RefSeq Size: 1267
• RefSeq ORF: 1044
• Locus ID: 344561
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 38.3 kDa
• Gene Summary: Orphan receptor. [UniProtKB/Swiss-Prot Function]