ECT2 (NM_018098) Human Tagged ORF Clone
CAT#: RC211319
ECT2 (Myc-DDK-tagged)-Human epithelial cell transforming sequence 2 oncogene (ECT2)
"NM_018098" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ECT2 |
Synonyms | ARHGEF31 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC211319 representing NM_018098
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAAAATAGTGTATTAACATCCACTACTGGGAGGACTAGCTTGGCAGACTCTTCCATTTTTGATT CTAAAGTTACTGAGATTTCCAAGGAAAACTTACTTATTGGATCTACTTCATATGTAGAAGAAGAGATGCC TCAGATTGAAACAAGAGTGATATTGGTTCAAGAAGCTGGAAAACAAGAAGAACTTATAAAAGCCTTAAAG GACATTAAAGTGGGCTTTGTAAAGATGGAGTCAGTGGAAGAATTTGAAGGTTTGGATTCTCCGGAATTTG AAAATGTATTTGTAGTCACGGACTTTCAGGATTCTGTCTTTAATGACCTCTACAAGGCTGATTGTAGAGT TATTGGACCACCAGTTGTATTAAATTGTTCACAAAAAGGAGAGCCTTTGCCATTTTCATGTCGCCCGTTG TATTGTACAAGTATGATGAATCTAGTACTATGCTTTACTGGATTTAGGAAAAAAGAAGAACTAGTCAGGT TGGTGACATTGGTCCATCACATGGGTGGAGTTATTCGAAAAGACTTTAATTCAAAAGTTACACATTTGGT GGCAAATTGTACACAAGGAGAAAAATTCAGGGTTGCTGTGAGTCTAGGTACTCCAATTATGAAGCCAGAA TGGATTTATAAAGCTTGGGAAAGGCGGAATGAACAGGATTTCTATGCAGCAGTTGATGACTTTAGAAATG AATTTAAAGTTCCTCCATTTCAAGATTGTATTTTAAGTTTCCTGGGATTTTCAGATGAAGAGAAAACCAA TATGGAAGAAATGACTGAAATGCAAGGAGGTAAATATTTACCGCTTGGAGATGAAAGATGCACTCACCTT GTAGTTGAAGAGAATATAGTAAAAGATCTTCCCTTTGAACCTTCAAAGAAACTTTATGTTGTCAAGCAAG AGTGGTTCTGGGGAAGCATTCAAATGGATGCCCGAGCTGGAGAAACTATGTATTTATATGAAAAGGCAAA TACTCCTGAGCTCAAGAAATCAGTGTCAATGCTTTCTCTAAATACCCCTAACAGCAATCGCAAACGACGT CGTTTAAAAGAAACACTTGCTCAGCTTTCAAGAGAGACAGACGTGTCACCATTTCCACCCCGTAAGCGCC CATCAGCTGAGCATTCCCTTTCCATAGGGTCACTCCTAGATATCTCCAACACACCAGAGTCTAGCATTAA CTATGGAGACACCCCAAAGTCTTGTACTAAGTCTTCTAAAAGCTCCACTCCAGTTCCTTCAAAGCAGTCA GCAAGGTGGCAAGTTGCAAAAGAGCTTTATCAAACTGAAAGTAATTATGTTAATATATTGGCAACAATTA TTCAGTTATTTCAAGTACCATTGGAAGAGGAAGGACAACGTGGTGGACCTATCCTTGCACCAGAGGAGAT TAAGACTATTTTTGGTAGCATCCCAGATATCTTTGATGTACACACTAAGATAAAGGATGATCTTGAAGAC CTTATAGTTAATTGGGATGAGAGCAAAAGCATTGGTGACATTTTTCTGAAATATTCAAAAGATTTGGTAA AAACCTACCCTCCCTTTGTAAACTTCTTTGAAATGAGCAAGGAAACAATTATTAAATGTGAAAAACAGAA ACCAAGATTTCATGCTTTTCTCAAGATAAACCAAGCAAAACCAGAATGTGGACGGCAGAGCCTTGTTGAA CTTCTTATCCGACCAGTACAGAGGTTACCCAGTGTTGCATTACTTTTAAATGATCTTAAGAAGCATACAG CTGATGAAAATCCAGACAAAAGCACTTTAGAAAAAGCTATTGGATCACTGAAGGAAGTAATGACGCATAT TAATGAGGATAAGAGAAAAACAGAAGCTCAAAAGCAAATTTTTGATGTTGTTTATGAAGTAGATGGATGC CCAGCTAATCTTTTATCTTCTCACCGAAGCTTAGTACAGCGGGTTGAAACAATTTCTCTAGGTGAGCACC CCTGTGACAGAGGAGAACAAGTAACTCTCTTCCTCTTCAATGATTGCCTAGAGATAGCAAGAAAACGGCA CAAGGTTATTGGCACTTTTAGGAGTCCTCATGGCCAAACCCGACCCCCAGCTTCTCTTAAGCATATTCAC CTAATGCCTCTTTCTCAGATTAAGAAGGTATTGGACATAAGAGAGACAGAAGATTGCCATAATGCTTTTG CCTTGCTTGTGAGGCCACCAACAGAGCAGGCAAATGTGCTACTCAGTTTCCAGATGACATCAGATGAACT TCCAAAAGAAAACTGGCTAAAGATGCTGTGTCGACATGTAGCTAACACCATTTGTAAAGCAGATGCTGAG AATCTTATTTATACTGCTGATCCAGAATCCTTTGAAGTAAATACAAAAGATATGGACAGTACATTGAGTA GAGCATCAAGAGCAATAAAAAAGACTTCAAAAAAGGTTACAAGAGCATTCTCTTTCTCCAAAACTCCAAA AAGAGCTCTTCGAAGGGCTCTTATGACATCCCACGGCTCAGTGGAGGGAAGAAGTCCTTCCAGCAATGAT AAGCATGTAATGAGTCGTCTTTCTAGCACATCATCATTAGCAGGTATCCCTTCTCCCTCCCTTGTCAGCC TTCCTTCCTTCTTTGAAAGGAGAAGTCATACGTTAAGTAGATCTACAACTCATTTGATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211319 representing NM_018098
Red=Cloning site Green=Tags(s) MAENSVLTSTTGRTSLADSSIFDSKVTEISKENLLIGSTSYVEEEMPQIETRVILVQEAGKQEELIKALK DIKVGFVKMESVEEFEGLDSPEFENVFVVTDFQDSVFNDLYKADCRVIGPPVVLNCSQKGEPLPFSCRPL YCTSMMNLVLCFTGFRKKEELVRLVTLVHHMGGVIRKDFNSKVTHLVANCTQGEKFRVAVSLGTPIMKPE WIYKAWERRNEQDFYAAVDDFRNEFKVPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKYLPLGDERCTHL VVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMYLYEKANTPELKKSVSMLSLNTPNSNRKRR RLKETLAQLSRETDVSPFPPRKRPSAEHSLSIGSLLDISNTPESSINYGDTPKSCTKSSKSSTPVPSKQS ARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGGPILAPEEIKTIFGSIPDIFDVHTKIKDDLED LIVNWDESKSIGDIFLKYSKDLVKTYPPFVNFFEMSKETIIKCEKQKPRFHAFLKINQAKPECGRQSLVE LLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGC PANLLSSHRSLVQRVETISLGEHPCDRGEQVTLFLFNDCLEIARKRHKVIGTFRSPHGQTRPPASLKHIH LMPLSQIKKVLDIRETEDCHNAFALLVRPPTEQANVLLSFQMTSDELPKENWLKMLCRHVANTICKADAE NLIYTADPESFEVNTKDMDSTLSRASRAIKKTSKKVTRAFSFSKTPKRALRRALMTSHGSVEGRSPSSND KHVMSRLSSTSSLAGIPSPSLVSLPSFFERRSHTLSRSTTHLI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_018098 |
ORF Size | 2649 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018098.4, NM_018098.5, NP_060568.3 |
RefSeq Size | 3916 bp |
RefSeq ORF | 2652 bp |
Locus ID | 1894 |
Cytogenetics | 3q26.31 |
Domains | RhoGEF, BRCT |
Protein Families | Druggable Genome |
MW | 99.9 kDa |
Gene Summary | 'The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC113779 | ECT2 (untagged)-Human epithelial cell transforming sequence 2 oncogene (ECT2) |
USD 760.00 |
|
SC318356 | ECT2 (untagged)-Human epithelial cell transforming sequence 2 oncogene (ECT2) |
USD 760.00 |
|
RG211319 | ECT2 (GFP-tagged) - Human epithelial cell transforming sequence 2 oncogene (ECT2) |
USD 460.00 |
|
RC211319L1 | Lenti ORF clone of Human epithelial cell transforming sequence 2 oncogene (ECT2), Myc-DDK-tagged |
USD 620.00 |
|
RC211319L2 | Lenti ORF clone of Human epithelial cell transforming sequence 2 oncogene (ECT2), mGFP tagged |
USD 620.00 |
|
RC211319L3 | Lenti ORF clone of Human epithelial cell transforming sequence 2 oncogene (ECT2), Myc-DDK-tagged |
USD 768.00 |
|
RC211319L4 | Lenti ORF clone of Human epithelial cell transforming sequence 2 oncogene (ECT2), mGFP tagged |
USD 620.00 |
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