HGFAC (NM_001528) Human Tagged ORF Clone

CAT#: RC211348

HGFAC (Myc-DDK-tagged)-Human HGF activator (HGFAC)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HGFAC
• Synonyms: HGFA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211348 representing NM_001528
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGCTGGGCCTGGGTCCCCAGCCCCTGGCCCCCACCGGGGCTGGGCCCCTTCCTCCTCCTCCTCC
  TGCTGCTGCTGCTGCTGCCACGGGGGTTCCAGCCCCAGCCTGGCGGGAACCGTACGGAGTCCCCAGAACC
  TAATGCCACAGCGACCCCTGCGATCCCCACTATCCTGGTGACCTCTGTGACCTCTGAGACCCCAGCAACA
  AGTGCTCCAGAGGCAGAGGGACCCCAAAGTGGGGGGCTCCCGCCCCCGCCCAGGGCAGTTCCCTCGAGCA
  GTAGCCCCCAGGCCCAAGCACTCACCGAGGACGGGAGGCCCTGCAGGTTCCCCTTCCGCTACGGGGGCCG
  CATGCTGCATGCCTGCACTTCGGAGGGCAGTGCACACAGGAAGTGGTGTGCCACAACTCACAACTACGAC
  CGGGACAGGGCCTGGGGCTACTGTGTGGAGGCCACCCCGCCTCCAGGGGGCCCAGCTGCCCTGGATCCCT
  GTGCCTCCGGCCCCTGCCTCAATGGAGGCTCCTGCTCCAATACCCAGGACCCCCAGTCCTATCACTGCAG
  CTGCCCCCGGGCCTTCACCGGCAAGGACTGCGGCACAGAGAAATGCTTTGATGAGACCCGCTACGAGTAC
  CTGGAGGGGGGCGACCGCTGGGCCCGCGTGCGCCAGGGCCACGTGGAACAGTGCGAGTGCTTCGGGGGCC
  GGACCTGGTGCGAAGGCACCCGACATACAGCTTGTCTGAGCAGCCCTTGCCTGAACGGGGGCACCTGCCA
  CCTGATCGTGGCCACCGGGACCACCGTGTGTGCCTGCCCACCAGGCTTCGCTGGACGGCTCTGCAACATC
  GAGCCTGATGAGCGCTGCTTCTTGGGGAACGGCACTGGGTACCGTGGCGTGGCCAGCACCTCAGCCTCGG
  GCCTCAGCTGCCTGGCCTGGAACTCCGATCTGCTCTACCAGGAGCTGCACGTGGACTCCGTGGGCGCCGC
  GGCCCTGCTGGGCCTGGGCCCCCATGCCTACTGCCGGAATCCGGACAATGACGAGAGGCCCTGGTGCTAC
  GTGGTGAAGGACAGCGCGCTCTCCTGGGAGTACTGCCGCCTGGAGGCCTGCGAATCCCTCACCAGAGTCC
  AACTGTCACCGGATCTCCTGGCGACCCTGCCTGAGCCAGCCTCCCCGGGGCGCCAGGCCTGCGGCAGGAG
  GCACAAGAAGAGGACGTTCCTGCGGCCACGTATCATCGGCGGCTCCTCCTCGCTGCCCGGCTCGCACCCC
  TGGCTGGCCGCCATCTACATCGGGGACAGCTTCTGCGCCGGGAGCCTGGTCCACACCTGCTGGGTGGTGT
  CGGCCGCCCACTGCTTCTCCCACAGCCCCCCCAGGGACAGCGTCTCCGTGGTGCTGGGCCAGCACTTCTT
  CAACCGCACGACGGACGTGACGCAGACCTTCGGCATCGAGAAGTACATCCCGTACACCCTGTACTCGGTG
  TTCAACCCCAGCGACCACGACCTCGTCCTGATCCGGCTGAAGAAGAAAGGGGACCGCTGTGCCACACGCT
  CGCAGTTCGTGCAGCCCATCTGCCTGCCCGAGCCCGGCAGCACCTTCCCCGCAGGACACAAGTGCCAGAT
  TGCGGGCTGGGGCCACTTGGATGAGAACGTGAGCGGCTACTCCAGCTCCCTGCGGGAGGCCCTGGTCCCC
  CTGGTCGCCGACCACAAGTGCAGCAGCCCTGAGGTCTACGGCGCCGACATCAGCCCCAACATGCTCTGTG
  CCGGCTACTTCGACTGCAAGTCCGACGCCTGCCAGGGGGACTCAGGGGGGCCCCTGGCCTGCGAGAAGAA
  CGGCGTGGCTTACCTCTACGGCATCATCAGCTGGGGTGACGGCTGCGGGCGGCTCCACAAGCCGGGGGTC
  TACACCCGCGTGGCCAACTATGTGGACTGGATCAACGACCGGATACGGCCTCCCAGGCGGCTTGTGGCTC
  CCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211348 representing NM_001528
  Red=Cloning site Green=Tags(s)
  
  MGRWAWVPSPWPPPGLGPFLLLLLLLLLLPRGFQPQPGGNRTESPEPNATATPAIPTILVTSVTSETPAT
  SAPEAEGPQSGGLPPPPRAVPSSSSPQAQALTEDGRPCRFPFRYGGRMLHACTSEGSAHRKWCATTHNYD
  RDRAWGYCVEATPPPGGPAALDPCASGPCLNGGSCSNTQDPQSYHCSCPRAFTGKDCGTEKCFDETRYEY
  LEGGDRWARVRQGHVEQCECFGGRTWCEGTRHTACLSSPCLNGGTCHLIVATGTTVCACPPGFAGRLCNI
  EPDERCFLGNGTGYRGVASTSASGLSCLAWNSDLLYQELHVDSVGAAALLGLGPHAYCRNPDNDERPWCY
  VVKDSALSWEYCRLEACESLTRVQLSPDLLATLPEPASPGRQACGRRHKKRTFLRPRIIGGSSSLPGSHP
  WLAAIYIGDSFCAGSLVHTCWVVSAAHCFSHSPPRDSVSVVLGQHFFNRTTDVTQTFGIEKYIPYTLYSV
  FNPSDHDLVLIRLKKKGDRCATRSQFVQPICLPEPGSTFPAGHKCQIAGWGHLDENVSGYSSSLREALVP
  LVADHKCSSPEVYGADISPNMLCAGYFDCKSDACQGDSGGPLACEKNGVAYLYGIISWGDGCGRLHKPGV
  YTRVANYVDWINDRIRPPRRLVAPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001528
• ORF Size: 1965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001528.1, NM_001528.2, NM_001528.3, NP_001519.1
• RefSeq Size: 2038 bp
• RefSeq ORF: 1968 bp
• Locus ID: 3083
• Cytogenetics: 4p16.3
• Domains: KR, FN1, FN2, Tryp_SPc, EGF, EGF
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• MW: 70.68 kDa
• Gene Summary: 'This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]'