FAM107B (NM_031453) Human Tagged ORF Clone

CAT#: RC211413

FAM107B (Myc-DDK-tagged)-Human family with sequence similarity 107, member B (FAM107B)

Product Images

Western blot validation of overexpression lysate (Cat# LY410525) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211413 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FAM107B protein (Cat# TP311413). The protein was produced from HEK293T cells transfected with FAM107B cDNA clone (Cat# RC211413) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM107B
• Synonyms: C10orf45; HITS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211413 representing NM_031453
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACGTGGAAAGCAAGACTCACCAAAAGACTGAAGTCTCCCTCTAGAAGCATGCATCCATTTCCGT
  GCTCAGCTCTGCTCGCCTGTTTTGGGAATACGAGGGAGAGTGCTTCCTTCAATCAGTCCGGCGTGGCTGA
  TACTCATTCCACCGTCCGTGTGCAGCCTGTGGCCAAAGCAGGCAGACAGCCAAGACACCCAAGCGCAGAA
  GGAGCTCCAGAGAAAAGGCAAGATTCGAGCACCCATGCAGAGAGAAATGGCAGTGCGAATCGGAATTCAA
  GTCACCGCACTGCGGCCCAGCCCGCGGAGACGCCTGAAGATGTGCCCGGGTCCCTGGATGATGGGGCGGA
  CTGTGAAGCAGTGGTGTTTCACGCTTCCATCCCCAGACCATCAATTATTGACACGCCCAAGGAAGAAGAA
  TTTCGAGAAGAACCTAAATGCCTCGAGCTGGAGCAGAAAATGACATCAGACAGCCCACCTGAGGATATTG
  ACCATAAGGACTCATATCTCATTACAAGAAGCATCATGGCCGAGCCAGACTACATAGAAGATGACAATCC
  TGAACTCATTAGGCCTCAGAAACTGATCAATCCTGTAAAAACCTCCCGGAACCATCAAGATCTTCACAGA
  GAACTTCTTATGAATCAAAAAAGGGGTCTTGCTCCTCAGAACAAACCAGAATTGCAGAAGGTGATGGAAA
  AAAGAAAACGAGACCAAGTAATAAAGCAGAAGGAAGAAGAAGCACAGAAGAAGAAATCTGACTTGGAAAT
  AGAGCTATTAAAACGGCAGCAGAAGTTGGAGCAGCTTGAACTTGAGAAGCAGAAATTGCAAGAAGAGCAA
  GAAAATGCCCCCGAGTTTGTGAAGGTGAAAGGCAATCTCAGGAGAACAGGCCAAGAAGTCGCCCAAGCCC
  AGGAGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211413 representing NM_031453
  Red=Cloning site Green=Tags(s)
  
  MSTWKARLTKRLKSPSRSMHPFPCSALLACFGNTRESASFNQSGVADTHSTVRVQPVAKAGRQPRHPSAE
  GAPEKRQDSSTHAERNGSANRNSSHRTAAQPAETPEDVPGSLDDGADCEAVVFHASIPRPSIIDTPKEEE
  FREEPKCLELEQKMTSDSPPEDIDHKDSYLITRSIMAEPDYIEDDNPELIRPQKLINPVKTSRNHQDLHR
  ELLMNQKRGLAPQNKPELQKVMEKRKRDQVIKQKEEEAQKKKSDLEIELLKRQQKLEQLELEKQKLQEEQ
  ENAPEFVKVKGNLRRTGQEVAQAQES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031453
• ORF Size: 918 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031453.2, NM_031453.3, NP_113641.2
• RefSeq Size: 3785
• RefSeq ORF: 921
• Locus ID: 83641
• MW: 34.6 kDa