AZI2 (NM_022461) Human Tagged ORF Clone

CAT#: RC211435

AZI2 (Myc-DDK-tagged)-Human 5-azacytidine induced 2 (AZI2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY411676) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211435 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AZI2 protein (Cat# TP311435). The protein was produced from HEK293T cells transfected with AZI2 cDNA clone (Cat# RC211435) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AZI2
• Synonyms: AZ2; NAP1; TILP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211435 representing NM_022461
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCACTGGTAGAAGATGATATCTGTATTCTGAATCATGAAAAAGCCCATAAGAGAGATACAGTGA
  CTCCAGTTTCAATATATTCAGGAGATGAATCTGTTGCTTCCCATTTTGCTCTTGTCACTGCATATGAAGA
  CATCAAAAAACGACTTAAGGATTCAGAGAAAGAGAACTCTTTGTTAAAGAAGAGAATAAGATTTTTGGAA
  GAAAAGCTAATAGCTCGATTTGAAGAAGAAACAAGTTCCGTGGGACGAGAACAAGTAAATAAGGCCTATC
  ATGCATATCGAGAGGTTTGCATTGATAGAGATAATTTGAAGAGCAAACTGGACAAAATGAATAAAGACAA
  CTCTGAATCTTTGAAAGTATTGAATGAGCAGCTACAATCTAAAGAAGTAGAACTCCTCCAGCTGAGGACA
  GAGGTGGAAACTCAGCAGGTGATGAGGAATTTAAATCCACCTTCATCAAACTGGGAGGTGGAAAAGTTGA
  GCTGTGACCTGAAGATCCATGGTTTGGAACAAGAGCTGGAACTGATGAGGAAAGAATGTAGCGATCTCAA
  AATAGAACTACAGAAAGCCAAACAAACGGATCCATATCAGGAAGACAATCTGAAGAGCAGAGATCTCCAA
  AAACTAAGCATTTCAAGTGATAATATGCAGCATGCATACTGGGAACTGAAGAGAGAAATGTCTAATTTAC
  ATCTGGTGACTCAAGTACAAGCTGAACTACTAAGAAAACTGAAAACCTCAACTGCAATCAAGAAAGCCTG
  TGCCCCTGTAGGATGCAGTGAAGACCTTGGAAGAGACAGCACAAAACTGCACTTGATGAATTTTACTGCA
  ACATACACAAGACATCCCCCTCTCTTACCAAATGGCAAAGCTCTTTGTCATACCACATCTTCCCCTTTAC
  CAGGAGATGTAAAGGTTTTATCAGAGAAAGCAATCCTCCAATCATGGACAGACAATGAGAGATCCATTCC
  TAATGATGGTACATGCTTTCAGGAACACAGTTCTTATGGCAGAAATTCTCTGGAAGACAATTCCTGGGTA
  TTTCCAAGTCCTCCTAAATCAAGTGAGACAGCATTTGGGGAAACTAAAACTAAAACTTTGCCTTTACCCA
  ACCTTCCACCACTGCATTACTTGGATCAACATAATCAGAACTGCCTTTATAAGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211435 representing NM_022461
  Red=Cloning site Green=Tags(s)
  
  MDALVEDDICILNHEKAHKRDTVTPVSIYSGDESVASHFALVTAYEDIKKRLKDSEKENSLLKKRIRFLE
  EKLIARFEEETSSVGREQVNKAYHAYREVCIDRDNLKSKLDKMNKDNSESLKVLNEQLQSKEVELLQLRT
  EVETQQVMRNLNPPSSNWEVEKLSCDLKIHGLEQELELMRKECSDLKIELQKAKQTDPYQEDNLKSRDLQ
  KLSISSDNMQHAYWELKREMSNLHLVTQVQAELLRKLKTSTAIKKACAPVGCSEDLGRDSTKLHLMNFTA
  TYTRHPPLLPNGKALCHTTSSPLPGDVKVLSEKAILQSWTDNERSIPNDGTCFQEHSSYGRNSLEDNSWV
  FPSPPKSSETAFGETKTKTLPLPNLPPLHYLDQHNQNCLYKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022461
• ORF Size: 1176 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022461.1, NM_022461.2, NM_022461.3, NM_022461.4, NP_071906.1
• RefSeq Size: 3113 bp
• RefSeq ORF: 1179 bp
• Locus ID: 64343
• Protein Pathways: RIG-I-like receptor signaling pathway
• MW: 44.8 kDa
• Gene Summary: AZI2, or NAP1, contributes to the activation of NFKB (see MIM 164011)-dependent gene expression by activating IKK-related kinases, such as NAK (TBK1; MIM 604834) (Fujita et al., 2003 [PubMed 14560022]). [supplied by OMIM, Mar 2008]