TRIP12 (NM_004238) Human Tagged ORF Clone

CAT#: RC211510

TRIP12 (Myc-DDK-tagged)-Human thyroid hormone receptor interactor 12 (TRIP12)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIP12
• Synonyms: MRD49; TRIP-12; ULF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211510 representing NM_004238
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAACCGGCCTAATAACAATCCAGGGGGGTCACTGCGACGTTCACAGAGGAACACTGCCGGGGCCC
  AACCACAAGACGACTCAATAGGAGGAAGAAGCTGCAGTTCATCATCTGCTGTGATAGTTCCACAACCAGA
  GGATCCAGACAGAGCCAATACTTCAGAAAGACAAAAAACGGGGCAGGTGCCTAAGAAAGACAATTCTCGA
  GGAGTGAAGCGCAGTGCTAGTCCAGACTACAACAGGACCAATTCTCCTAGCTCTGCAAAAAAACCAAAAG
  CACTTCAGCATACTGAATCTCCCTCAGAAACAAATAAGCCACATAGTAAGTCAAAGAAGAGACATTTAGA
  CCAGGAGCAACAACTGAAATCTGCACAATCACCATCAACAAGCAAGGCTCATACCAGGAAGAGTGGGGCC
  ACTGGCGGTTCACGGAGTCAGAAAAGAAAAAGGACAGAGAGTTCTTGTGTAAAGAGTGGCTCCGGGTCTG
  AATCAACTGGTGCAGAAGAGAGATCTGCGAAACCTACCAAGCTGGCTTCAAAATCAGCCACCTCAGCCAA
  AGCTGGGTGTAGCACCATCACTGATTCTTCTTCTGCTGCCTCTACTTCCTCCTCGTCTTCTGCTGTAGCC
  TCGGCCTCCTCCACTGTACCACCAGGTGCCAGAGTGAAACAAGGAAAAGATCAGAACAAGGCCAGGCGTT
  CCCGTTCAGCGTCCAGTCCCAGCCCCAGAAGAAGTAGCAGGGAAAAGGAACAGAGTAAAACTGGTGGCTC
  TTCAAAATTTGATTGGGCTGCTCGTTTCAGCCCTAAAGTTAGCCTTCCTAAAACAAAACTGTCTCTTCCA
  GGGTCTTCTAAGTCAGAGACATCAAAACCTGGACCTTCTGGATTACAGGCCAAATTAGCAAGTTTAAGAA
  AATCTACGAAGAAACGCAGTGAGTCTCCACCTGCTGAGCTCCCCAGTTTGAGGCGGAGCACACGCCAAAA
  GACCACGGGCTCCTGTGCTAGTACCAGTCGGCGAGGCTCTGGCCTGGGCAAAAGAGGAGCAGCTGAAGCT
  CGTCGACAGGAGAAAATGGCAGACCCTGAAAGCAACCAGGAGGCAGTAAATTCTTCAGCTGCTCGGACAG
  ATGAAGCTCCCCAAGGAGCTGCAGGGGCTGTTGGCATGACCACCTCTGGGGAGAGTGAATCAGATGATTC
  CGAGATGGGACGTTTGCAAGCTTTGTTAGAGGCAAGGGGTCTTCCCCCTCACCTATTTGGTCCTCTTGGT
  CCTCGGATGTCACAGCTTTTCCATAGAACAATTGGAAGTGGAGCTAGTTCTAAGGCCCAGCAGCTACTAC
  AAGGATTGCAAGCCAGTGATGAAAGTCAACAGCTTCAGGCAGTTATTGAGATGTGTCAGTTACTGGTCAT
  GGGAAATGAGGAGACACTGGGAGGGTTTCCTGTCAAGAGTGTTGTTCCAGCTTTGATTACGTTACTTCAG
  ATGGAGCACAATTTTGATATTATGAACCATGCTTGTCGAGCCTTAACATACATGATGGAAGCACTTCCTC
  GATCTTCTGCTGTTGTAGTAGATGCTATTCCTGTCTTTTTAGAAAAGCTGCAAGTTATTCAGTGTATTGA
  TGTGGCAGAGCAGGCCTTGACTGCCTTGGAGATGTTGTCACGGAGACATAGTAAAGCCATTCTACAGGCG
  GGTGGTTTGGCAGACTGCTTGCTGTACCTAGAATTCTTCAGCATAAATGCCCAAAGAAATGCATTAGCAA
  TTGCAGCTAATTGCTGCCAGAGTATCACGCCAGATGAATTTCATTTTGTGGCAGATTCACTCCCATTGCT
  AACCCAAAGGCTAACACATCAGGATAAAAAGTCAGTAGAAAGCACTTGCCTTTGTTTTGCACGCCTAGTG
  GACAACTTCCAGCATGAGGAGAATTTACTCCAGCAGGTTGCTTCCAAAGATCTGCTTACAAATGTTCAAC
  AGCTGTTGGTAGTGACTCCACCCATTTTAAGTTCTGGGATGTTTATAATGGTGGTTCGCATGTTTTCTCT
  GATGTGTTCCAACTGTCCAACTTTAGCTGTTCAACTTATGAAACAAAACATTGCAGAAACGCTTCACTTT
  CTCCTGTGTGGTGCCTCCAATGGAAGTTGTCAGGAACAGATTGATCTTGTTCCACGAAGCCCTCAAGAGT
  TGTATGAACTGACATCTCTGATTTGTGAACTTATGCCATGTTTACCAAAAGAAGGCATTTTTGCAGTTGA
  TACCATGTTGAAGAAGGGAAATGCACAGAACACAGATGGTGCGATATGGCAGTGGCGTGATGATCGGGGC
  CTCTGGCATCCATATAACAGGATTGACAGCCGGATCATTGAGCAAATCAATGAGGACACGGGAACAGCAC
  GTGCCATTCAGAGAAAACCTAACCCGTTAGCCAATAGTAACACTAGTGGATATTCAGAGTCAAAGAAGGA
  TGATGCTCGAGCACAGCTTATGAAAGAGGATCCGGAACTGGCTAAGTCTTTTATTAAGACATTATTTGGT
  GTTCTTTATGAAGTGTATAGTTCCTCAGCAGGACCTGCGGTCAGACATAAGTGCCTTAGAGCAATTCTTA
  GGATAATTTATTTTGCGGATGCTGAACTTCTGAAGGATGTTCTGAAAAATCATGCTGTTTCAAGTCACAT
  TGCTTCCATGCTGTCAAGCCAAGACCTGAAGATAGTAGTGGGAGCACTTCAGATGGCAGAAATTTTAATG
  CAGAAGTTACCTGATATTTTTAGTGTTTACTTCAGAAGAGAAGGTGTAATGCATCAAGTAAAACACTTAG
  CAGAATCAGAGTCTTTGTTGACAAGTCCACCAAAGGCATGTACGAATGGATCGGGATCCATGGGATCCAC
  AACTTCAGTCAGCAGTGGGACAGCCACAGCTGCCACTCATGCTGCAGCTGACTTGGGATCACCCAGCTTG
  CAGCACAGCAGGGATGATTCTTTAGATCTCAGCCCTCAAGGTCGATTAAGTGATGTTCTAAAGAGAAAAC
  GACTGCCAAAACGAGGGCCAAGAAGGCCAAAGTACTCACCTCCAAGAGATGATGACAAAGTAGACAATCA
  AGCTAAAAGCCCCACCACTACTCAGTCACCTAAATCTTCTTTCCTGGCAAGCTTGAATCCAAAAACATGG
  GGAAGGTTAAGTACACAGTCCAACAGCAACAACATTGAGCCAGCACGGACTGCGGGAGGTAGTGGCCTTG
  CCAGGGCTGCCTCAAAGGATACCATCTCCAATAATAGAGAAAAAATTAAAGGTTGGATTAAGGAGCAGGC
  ACATAAATTTGTAGAACGTTATTTCAGTTCTGAGAATATGGATGGAAGCAACCCTGCATTGAATGTCCTT
  CAGAGACTTTGTGCTGCAACCGAACAACTCAACCTCCAGGTGGATGGTGGAGCTGAGTGCCTTGTAGAAA
  TCCGTAGCATAGTCTCAGAGTCAGATGTTTCATCATTTGAAATCCAACATAGTGGATTTGTGAAGCAGCT
  GTTGCTTTATTTGACATCTAAAAGTGAAAAGGATGCTGTGAGCAGAGAGATCAGATTAAAGCGATTTCTT
  CATGTATTTTTTTCTTCTCCACTTCCTGGAGAAGAGCCCATTGGAAGAGTGGAACCAGTGGGTAATGCAC
  CTTTGTTGGCATTAGTTCACAAGATGAACAACTGCCTCAGCCAGATGGAACAATTTCCAGTCAAAGTACA
  TGATTTCCCTAGTGGAAATGGGACAGGAGGCAGCTTTTCTCTCAACAGAGGATCACAGGCTTTAAAATTT
  TTCAACACACATCAATTAAAATGCCAGTTACAAAGGCATCCAGACTGTGCAAATGTGAAGCAGTGGAAGG
  GTGGACCTGTCAAGATTGACCCTCTGGCTTTGGTACAAGCCATCGAGAGATACCTTGTAGTTAGAGGGTA
  TGGAAGAGTAAGAGAAGATGATGAAGACAGCGATGACGATGGATCAGATGAGGAAATAGATGAGTCTCTG
  GCTGCTCAGTTCCTAAATTCAGGAAATGTAAGACACAGGCTGCAGTTTTATATTGGAGAACATTTGCTGC
  CGTATAACATGACTGTGTATCAGGCAGTACGGCAGTTTAGTATACAGGCTGAAGATGAAAGAGAATCCAC
  AGATGATGAGAGCAATCCTCTAGGCAGAGCTGGTATTTGGACAAAGACTCATACAATATGGTATAAACCT
  GTGAGAGAGGATGAAGAAAGTAATAAAGATTGTGTTGGTGGTAAAAGAGGAAGAGCCCAAACAGCTCCAA
  CGAAAACTTCCCCTAGAAATGCAAAAAAGCATGATGAGTTATGGCACGATGGAGTGTGCCCATCAGTATC
  AAATCCTTTAGAAGTTTACCTCATTCCCACACCACCTGAAAATATAACATTTGAAGACCCGTCATTAGAT
  GTGATCCTTCTTTTAAGAGTTTTACATGCTATCAGTCGATACTGGTATTACTTGTATGATAATGCAATGT
  GCAAGGAAATTATTCCAACTAGTGAATTTATTAACAGTAAGTTAACAGCAAAAGCAAATAGGCAACTTCA
  AGATCCTTTAGTAATCATGACAGGAAACATCCCAACATGGCTTACTGAGCTAGGAAAAACCTGCCCATTT
  TTCTTTCCTTTTGATACCCGGCAAATGCTTTTTTATGTAACTGCATTTGATCGGGACCGAGCAATGCAAA
  GATTACTTGATACCAACCCAGAAATCAACCAGTCTGATTCTCAAGATAGCAGAGTTGCACCTAGATTGGA
  TAGAAAAAAACGTACTGTGAACCGAGAGGAGCTGCTGAAACAGGCGGAGTCTGTGATGCAGGACCTCGGC
  AGCTCACGGGCCATGTTAGAAATCCAGTATGAAAATGAGGTTGGTACAGGTCTTGGGCCTACACTGGAGT
  TTTATGCGCTTGTATCTCAGGAACTACAGAGAGCTGACTTGGGTCTTTGGAGAGGTGAAGAAGTAACTCT
  TAGCAATCCAAAAGGGAGCCAAGAAGGGACCAAGTATATTCAAAACCTCCAGGGCCTGTTTGCGCTTCCC
  TTTGGTAGGACAGCAAAGCCAGCTCATATCGCAAAGGTTAAGATGAAGTTTCGCTTCTTAGGAAAATTAA
  TGGCCAAGGCTATCATGGATTTCAGATTGGTGGACCTTCCCCTTGGCTTACCCTTTTATAAATGGATGCT
  ACGGCAAGAAACTTCACTGACATCACACGATTTGTTTGACATCGACCCAGTTGTAGCCAGATCAGTTTAT
  CACCTAGAAGACATTGTCAGACAGAAGAAAAGACTTGAACAAGATAAATCCCAGACCAAAGAGAGTCTAC
  AGTATGCATTAGAAACCTTGACTATGAATGGCTGCTCAGTTGAAGATCTAGGACTGGATTTCACTCTGCC
  AGGGTTTCCCAATATCGAACTGAAGAAAGGAGGGAAGGATATACCAGTCACTATCCACAATTTAGAGGAG
  TATCTAAGACTGGTTATATTCTGGGCACTAAATGAAGGCGTTTCTAGGCAATTTGATTCGTTCAGAGATG
  GATTTGAATCAGTCTTCCCACTCAGTCATCTTCAGTACTTCTACCCGGAGGAACTGGATCAGCTCCTTTG
  TGGCAGTAAAGCAGACACTTGGGATGCAAAGACACTGATGGAATGCTGTAGGCCTGATCATGGTTATACT
  CATGACAGTCGGGCTGTGAAGTTTTTGTTTGAGATTCTCAGTAGTTTTGATAATGAGCAGCAGAGGTTAT
  TTCTCCAGTTTGTGACTGGTAGCCCAAGATTGCCTGTTGGAGGATTCCGGAGTTTGAATCCACCTTTGAC
  AATTGTCCGAAAGACGTTTGAATCAACAGAAAACCCAGATGACTTCTTGCCCTCTGTAATGACTTGTGTG
  AACTATCTTAAGTTGCCGGACTATTCAAGCATTGAGATAATGCGTGAAAAACTGTTGATAGCAGCAAGAG
  AAGGGCAGCAGTCGTTCCATCTTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211510 representing NM_004238
  Red=Cloning site Green=Tags(s)
  
  MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSR
  GVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGA
  TGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVA
  SASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLP
  GSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEA
  RRQEKMADPESNQEAVNSSAARTDEAPQGAAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLG
  PRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQ
  MEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQA
  GGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLV
  DNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHF
  LLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRG
  LWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFG
  VLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILM
  QKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSL
  QHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTW
  GRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVL
  QRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFL
  HVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKF
  FNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESL
  AAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKP
  VREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLD
  VILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPF
  FFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLG
  SSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALP
  FGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVY
  HLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEE
  YLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYT
  HDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCV
  NYLKLPDYSSIEIMREKLLIAAREGQQSFHLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004238
• ORF Size: 5976 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004238.1, NM_004238.2, NP_004229.1
• RefSeq Size: 6428
• RefSeq ORF: 5979
• Locus ID: 9320
• Domains: HECT
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 220.3 kDa
• Gene Summary: The protein encoded by this gene is an E3 ubiquitin-protein ligase involved in the degradation of the p19ARF/ARF isoform of CDKN2A, a tumor suppressor. The encoded protein also plays a role in the DNA damage response by regulating the stability of USP7, which regulates tumor suppressor p53. [provided by RefSeq, Jan 2017]