NDST4 (NM_022569) Human Tagged ORF Clone

CAT#: RC211521

NDST4 (Myc-DDK-tagged)-Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4 (NDST4)

Product Images

Western blot validation of overexpression lysate (Cat# LY411635) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211521 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NDST4
• Synonyms: N-HSST; N-HSST 4; NDST-4; NHSST4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211521 representing NM_022569
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCTTATTGTGAAACTTCGGAGAAGTTTTCGAACATTGATTGTTCTCTTAGCTACCTTTTGCTTGG
  TGAGCATTGTCATTTCTGCCTATTTTCTCTACTCTGGCTACAAACAGGAAATGACACTTATTGAAACCAC
  TGCAGAAGCAGAATGCACTGACATCAAAATTCTACCATATAGGTCAATGGAGCTGAAAACAGTTAAACCT
  ATTGACACATCCAAAACGGACCCTACTGTCCTTCTCTTCGTGGAGAGCCAATACTCTCAACTCGGTCAAG
  ATATCATAGCTATTTTGGAGTCCAGCCGATTTCAGTACCACATGGTTATTGCCCCTGGAAAGGGAGATAT
  ACCTCCTCTTACAGATAATGGCAAAGGGAAATATACTTTAGTTATTTATGAAAATATTCTGAAGTATGTC
  AGCATGGACTCATGGAATCGAGAGCTTTTAGAAAAATACTGTGTGGAATACAGTGTTAGTATAATCGGTT
  TTCATAAAGCCAATGAGAACAGCTTACCAAGTACACAATTAAAAGGCTTTCCGTTAAACCTTTTCAATAA
  TCTAGCTCTAAAGGACTGTTTTGTTAACCCTCAATCTCCTTTGCTGCATATTACCAAAGCCCCCAAGGTT
  GAGAAAGGCCCTCTTCCTGGGGAAGACTGGACTATTTTCCAATATAATCATTCAACCTACCAGCCTGTAC
  TTTTAACTGAGTTACAGACAGAAAAATCCCTGTCATCCTTGTCTAGCAAAACACTCTTTGCAACGGTGAT
  TCAGGATCTGGGGCTTCATGATGGAATTCAGAGAGTACTTTTTGGCAACAACTTGAACTTTTGGCTGCAC
  AAGCTCATCTTCATAGATGCCATCTCCTTCTTGTCAGGGAAGAGGCTGACATTGTCCTTGGACAGGTACA
  TCCTTGTGGACATTGATGATATATTTGTTGGGAAAGAGGGAACAAGGATGAATGTCAAAGATGTGAAGGC
  ATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTTCAACCTTGGATTTTCAGGG
  AAGTTTTACCACACAGGGACTGAAGAGGAAGATGAAGGAGATGACCTTTTACTTCGGTCTGTGGATGAGT
  TCTGGTGGTTTCCTCACATGTGGAGCCACATGCAGCCCCACCTCTTCCACAACGAGTCATCTTTAGTAGA
  GCAGATGATTCTCAACAAGGAATTTGCACTGGAACATGGAATACCAATCAACATGGGCTATGCTGTGGCC
  CCACATCACTCAGGGGTCTACCCGGTTCACATTCAGCTGTATGCAGCTTGGAAGAAGGTCTGGGGTATTC
  AAGTCACCAGCACTGAAGAATATCCACATCTGAAACCTGCCCGGTACAGAAAGGGCTTCATTCACAATAG
  CATCATGGTCCTCCCTCGACAGACTTGTGGGTTGTTCACTCACACTATTTTCTACAAAGAATATCCAGGA
  GGACCCCAAGAACTGGATAAAAGTATCAGAGGAGGTGAACTTTTTCTCACAATCCTTCTAAACCCAATCA
  GCATTTTCATGACCCATTTATCAAACTATGGAAATGACCGCCTAGGGTTATATACCTTTGTGAACTTGGT
  CAACTTTGTGCAGAGCTGGACCAACCTGAAATTGCAAACTCTGCCTCCAGTGCAACTGGCCCACCAGTAT
  TTTGAGCTCTTCCCTGAGCAGAAAGACCCTCTATGGCAGAATCCATGTGATGATAAACGACACAAAGACA
  TCTGGTCCAGAGAGAAAACTTGTGACCACTTACCAAAATTTCTAGTAATTGGTCCACAAAAAACAGGAAC
  AACTGCACTTTATTTATTTCTTCTTATGCATCCTTCAATCATCAGCAATCTCCCTAGTCCAAAGACATTT
  GAGGAAGTTCAGTTCTTTAATGGCAACAACTATCACAAAGGAATAGACTGGTATATGGACTTTTTCCCTA
  CACCATCCAATACTACAAGTGACTTTCTGTTTGAAAAGAGTGCTAATTACTTCCATTCGGAAGAAGCTCC
  AAGACGAGCCGCATCTCTTGTCCCCAAAGCCAAGATCATCACCATCCTCATTGACCCCTCAGACAGGGCA
  TACTCTTGGTACCAGCACCAACGATCACATGAAGATCCAGCTGCTCTGAGGTTCAATTTCTATGAAGTTA
  TTTCAACAGGACATTGGGCTCCATCTGACTTAAAAACTTTGCAGAGAAGATGCCTAGTACCTGGATGGTA
  TGCAGTCCACATAGAAAGATGGCTAACTTACTTTGCTACTTCTCAGTTGCTAATTATTGATGGACAGCAG
  CTGAGATCTGACCCAGCTACTGTGATGGATGAAGTCCAGAAGTTTCTGGGAGTTACACCTCGTTATAATT
  ACTCTGAAGCACTAACGTTTGATCCCCAAAAGGGTTTTTGGTGTCAATTACTGGAAGGAGGAAAGACAAA
  ATGCCTTGGAAAAAGCAAAGGCCGAAAATATCCACCTATGGATCCAGAGTCTAGAACGTTCCTCTCTAAT
  TACTACCGAGATCATAATGTGGAACTATCCAAACTGCTACACAGACTGGGACAGCCTCTGCCATCGTGGC
  TGAGACAGGAACTGCAGAAAGTGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211521 representing NM_022569
  Red=Cloning site Green=Tags(s)
  
  MNLIVKLRRSFRTLIVLLATFCLVSIVISAYFLYSGYKQEMTLIETTAEAECTDIKILPYRSMELKTVKP
  IDTSKTDPTVLLFVESQYSQLGQDIIAILESSRFQYHMVIAPGKGDIPPLTDNGKGKYTLVIYENILKYV
  SMDSWNRELLEKYCVEYSVSIIGFHKANENSLPSTQLKGFPLNLFNNLALKDCFVNPQSPLLHITKAPKV
  EKGPLPGEDWTIFQYNHSTYQPVLLTELQTEKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNLNFWLH
  KLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSG
  KFYHTGTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINMGYAVA
  PHHSGVYPVHIQLYAAWKKVWGIQVTSTEEYPHLKPARYRKGFIHNSIMVLPRQTCGLFTHTIFYKEYPG
  GPQELDKSIRGGELFLTILLNPISIFMTHLSNYGNDRLGLYTFVNLVNFVQSWTNLKLQTLPPVQLAHQY
  FELFPEQKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVIGPQKTGTTALYLFLLMHPSIISNLPSPKTF
  EEVQFFNGNNYHKGIDWYMDFFPTPSNTTSDFLFEKSANYFHSEEAPRRAASLVPKAKIITILIDPSDRA
  YSWYQHQRSHEDPAALRFNFYEVISTGHWAPSDLKTLQRRCLVPGWYAVHIERWLTYFATSQLLIIDGQQ
  LRSDPATVMDEVQKFLGVTPRYNYSEALTFDPQKGFWCQLLEGGKTKCLGKSKGRKYPPMDPESRTFLSN
  YYRDHNVELSKLLHRLGQPLPSWLRQELQKVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022569
• ORF Size: 2616 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022569.1, NM_022569.2, NP_072091.1
• RefSeq Size: 3359
• RefSeq ORF: 2619
• Locus ID: 64579
• Protein Families: Transmembrane
• Protein Pathways: Heparan sulfate biosynthesis, Metabolic pathways
• MW: 100.5 kDa