KCNIP4 (NM_147183) Human Tagged ORF Clone

CAT#: RC211613

KCNIP4 (Myc-DDK-tagged)-Human Kv channel interacting protein 4 (KCNIP4), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY407778) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211613 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KCNIP4 protein (Cat# TP311613). The protein was produced from HEK293T cells transfected with KCNIP4 cDNA clone (Cat# RC211613) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNIP4
• Synonyms: CALP; KCHIP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211613 representing NM_147183
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTGGAAGGGCTTGAAATGATAGCAGTTCTGATCGTCATTGTGCTTTTTGTTAAATTATTGGAAC
  AGTTTGGGCTGATTGAAGCAGGTTTAGAAGACAGCGTGGAAGATGAACTGGAGATGGCCACCGTCAGGCA
  TCGGCCCGAAGCCCTTGAGCTTCTGGAAGCCCAGAGCAAATTTACCAAGAAAGAGCTTCAGATCCTTTAC
  AGAGGATTTAAGAACGAATGCCCCAGTGGTGTTGTTAATGAAGAAACCTTCAAAGAGATTTACTCGCAGT
  TCTTTCCACAGGGAGACTCTACAACATATGCACATTTTCTGTTCAATGCATTTGATACAGACCACAATGG
  AGCTGTGAGTTTCGAGGATTTCATCAAAGGTCTTTCCATTTTGCTCCGGGGGACAGTACAAGAAAAACTC
  AATTGGGCATTTAATCTGTATGACATAAATAAAGATGGCTACATCACTAAAGAGGAAATGCTTGATATAA
  TGAAAGCAATATACGATATGATGGGTAAATGTACATATCCTGTCCTCAAAGAAGATGCTCCCAGACAACA
  CGTTGAAACATTTTTTCAGAAAATGGACAAAAATAAAGATGGGGTTGTTACCATAGATGAGTTCATTGAA
  AGCTGCCAAAAAGATGAAAACATAATGCGCTCCATGCAGCTCTTTGAAAATGTGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211613 representing NM_147183
  Red=Cloning site Green=Tags(s)
  
  MNLEGLEMIAVLIVIVLFVKLLEQFGLIEAGLEDSVEDELEMATVRHRPEALELLEAQSKFTKKELQILY
  RGFKNECPSGVVNEETFKEIYSQFFPQGDSTTYAHFLFNAFDTDHNGAVSFEDFIKGLSILLRGTVQEKL
  NWAFNLYDINKDGYITKEEMLDIMKAIYDMMGKCTYPVLKEDAPRQHVETFFQKMDKNKDGVVTIDEFIE
  SCQKDENIMRSMQLFENVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_147183
• ORF Size: 687 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_147183.1, NM_147183.2, NM_147183.3, NP_671712.1
• RefSeq Size: 1373 bp
• RefSeq ORF: 690 bp
• Locus ID: 80333
• Domains: EFh
• Protein Families: Druggable Genome, Ion Channels: Other
• MW: 26.3 kDa
• Gene Summary: This gene encodes a member of the family of voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs), which belong to the recoverin branch of the EF-hand superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. This protein member also interacts with presenilin. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]