UTF1 (NM_003577) Human Tagged ORF Clone

CAT#: RC211682

UTF1 (Myc-DDK-tagged)-Human undifferentiated embryonic cell transcription factor 1 (UTF1)

Product Images

Western blot validation of overexpression lysate (Cat# LY401188) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211682 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UTF1
• Synonyms: OTTHUMP00000020786; undifferentiated embryonic cell transcription factor 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211682 representing NM_003577
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTCCGGCCCCGCAGGCCGCCCCCGCTCGCGCCCCCCGCGCCGCCCTCGCCCGCCAGCCCCGACC
  CCGAGCCGCGGACACCCGGAGACGCCCCGGGGACCCCGCCCCGGAGGCCCGCCTCGCCCAGCGCGCTGGG
  GGAACTCGGGTTGCCGGTGTCCCCGGGCTCGGCGCAGCGCACGCCCTGGAGCGCCCGGGAGACGGAGCTG
  CTGCTGGGGACGCTGCTGCAACCGGCCGTGTGGCGCGCGCTGCTCCTGGACCGCCGCCAGGCCCTGCCCA
  CCTACCGCCGCGTGTCGGCCGCGCTGGCCCAGCAGCAGGTGCGCCGCACCCCCGCGCAGTGCCGCCGCCG
  CTACAAGTTCCTTAAAGACAAGTTTCGCGAGGCGCACGGCCAGCCGCCCGGGCCCTTCGACGAGCAGATC
  CGGAAGCTCATGGGGCTGCTGGGCGACAACGGGCGCAAACGGCCTCGCCGCCGCTCCCCGGGGTCCGGGC
  GCCCCCAGCGCGCCCGCCGCCCGGTCCCCAACGCGCACGCGCCGGCTCCCAGCGAACCAGACGCCACCCC
  GCTGCCCACCGCCCGCGACCGCGACGCGGACCCCACCTGGACGCTCCGCTTCAGCCCGTCCCCACCGAAG
  TCTGCGGACGCCTCCCCCGCCCCCGGCTCCCCGCCAGCTCCCGCCCCGACCGCCCTCGCCACCTGCATCC
  CCGAGGACCGCGCGCCCGTCCGCGGCCCCGGGTCCCCGCCGCCACCCCCGGCCCGCGAAGACCCCGACTC
  GCCGCCCGGCCGCCCCGAGGACTGCGCGCCCCCTCCGGCCGCGCCCCCGTCGCTGAACACCGCCCTGCTG
  CAGACCCTGGGGCACCTGGGCGACATCGCGAACATCCTGGGCCCGCTGCGCGACCAGCTGCTGACCTTGA
  ACCAGCACGTGGAGCAGCTGCGCGGCGCCTTCGACCAGACAGTGTCCCTGGCCGTGGGCTTCATTCTGGG
  CAGCGCGGCCGCCGAGCGAGGGGTCCTCAGGGACCCGTGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211682 representing NM_003577
  Red=Cloning site Green=Tags(s)
  
  MLLRPRRPPPLAPPAPPSPASPDPEPRTPGDAPGTPPRRPASPSALGELGLPVSPGSAQRTPWSARETEL
  LLGTLLQPAVWRALLLDRRQALPTYRRVSAALAQQQVRRTPAQCRRRYKFLKDKFREAHGQPPGPFDEQI
  RKLMGLLGDNGRKRPRRRSPGSGRPQRARRPVPNAHAPAPSEPDATPLPTARDRDADPTWTLRFSPSPPK
  SADASPAPGSPPAPAPTALATCIPEDRAPVRGPGSPPPPPAREDPDSPPGRPEDCAPPPAAPPSLNTALL
  QTLGHLGDIANILGPLRDQLLTLNQHVEQLRGAFDQTVSLAVGFILGSAAAERGVLRDPCQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003577
• ORF Size: 1023 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003577.1, NM_003577.2, NP_003568.2
• RefSeq Size: 1157 bp
• RefSeq ORF: 1026 bp
• Locus ID: 8433
• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
• MW: 36.3 kDa
• Gene Summary: The protein encoded by this gene is a leucine zipper-containing transcriptional coactivator that may link the upstream activator ATF2 with the basal transcription complex. The encoded protein is closely associated with chromatin and is required for the proper differentiation of embryonic carcinoma and embryonic stem cells. Found nearly exclusively in pluripotent cells, this protein can also serve as a transcriptional repressor. [provided by RefSeq, Nov 2015]