BRSK1 (NM_032430) Human Tagged ORF Clone

CAT#: RC211767

BRSK1 (Myc-DDK-tagged)-Human BR serine/threonine kinase 1 (BRSK1)

Product Images

Western blot validation of overexpression lysate (Cat# LY410124) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211767 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRSK1
• Synonyms: hSAD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211767 representing NM_032430
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCCGGGGCCAAGGAGGGAGGTGGGGGCTCTCCCGCCTACCACCTCCCCCACCCCCACCCCCACC
  CACCCCAGCACGCCCAATATGTGGGCCCCTATCGGCTGGAGAAGACGCTGGGCAAAGGACAGACAGGGCT
  GGTTAAACTCGGGGTCCACTGCATCACGGGTCAGAAGGTCGCCATCAAGATCGTGAACCGGGAGAAGCTG
  TCGGAGTCGGTGCTGATGAAGGTGGAGCGGGAGATCGCCATCCTGAAGCTCATCGAACACCCACATGTCC
  TCAAGCTCCACGACGTCTACGAGAACAAGAAATATTTGTACCTGGTTCTGGAGCACGTCTCGGGGGGTGA
  GCTATTCGACTACCTGGTAAAGAAGGGGAGACTGACGCCCAAGGAGGCCCGAAAGTTCTTCCGCCAGATT
  GTGTCTGCGCTGGACTTCTGCCACAGCTACTCCATCTGCCACAGAGACCTAAAGCCCGAGAACCTGCTTT
  TGGATGAGAAAAACAACATCCGCATTGCAGACTTCGGCATGGCGTCCCTGCAGGTGGGGGACAGCCTCCT
  GGAGACCAGCTGCGGGTCCCCCCATTATGCGTGTCCAGAGGTGATTAAGGGGGAAAAATATGATGGCCGC
  CGGGCAGACATGTGGAGCTGTGGAGTCATCCTCTTCGCCCTGCTCGTGGGGGCTCTGCCCTTTGATGACG
  ACAACCTCCGCCAGCTGCTGGAGAAGGTGAAACGGGGCGTCTTCCACATGCCCCACTTCATTCCTCCAGA
  TTGCCAGAGCCTCCTGAGGGGAATGATCGAAGTGGAGCCCGAAAAAAGGCTCAGTCTGGAGCAAATTCAG
  AAACATCCTTGGTACCTAGGCGGGAAACACGAGCCAGACCCGTGCCTGGAGCCAGCCCCTGGCCGCCGGG
  TAGCCATGCGGAGCCTGCCATCCAACGGAGAGCTGGACCCCGACGTCCTAGAGAGCATGGCATCACTGGG
  CTGCTTCAGGGACCGCGAGAGGCTGCATCGCGAGCTGCGCAGTGAGGAGGAGAACCAAGAAAAGATGATA
  TATTATCTGCTTTTGGATCGGAAGGAGCGGTATCCCAGCTGTGAGGACCAGGACCTGCCTCCCCGGAATG
  ATGTTGACCCCCCCCGGAAGCGTGTGGATTCTCCCATGCTGAGCCGTCACGGGAAGCGGCGACCAGAGCG
  GAAGTCCATGGAAGTCCTGAGCATCACCGATGCCGGGGGTGGTGGCTCCCCTGTACCCACCCGACGGGCC
  TTGGAGATGGCCCAGCACAGCCAGAGATCCCGTAGCGTCAGTGGAGCCTCCACGGGTCTGTCCTCCAGCC
  CTCTAAGCAGCCCAAGGAGTCCGGTCTTTTCCTTTTCACCGGAGCCGGGGGCTGGAGATGAGGCTCGAGG
  CGGGGGCTCCCCGACTTCCAAAACGCAGACGCTGCCTTCTCGGGGCCCCAGGGGTGGGGGCGCCGGGGAG
  CAGCCCCCGCCCCCCAGTGCCCGCTCCACACCCCTGCCCGGCCCCCCAGGCTCCCCGCGCTCCTCTGGCG
  GGACCCCCTTGCACTCGCCTCTGCACACGCCCCGGGCCAGTCCCACCGGGACCCCGGGGACAACACCACC
  CCCCAGCCCCGGCGGTGGCGTCGGGGGAGCCGCCTGGAGGAGTCGTCTCAACTCCATCCGCAACAGCTTC
  CTGGGCTCCCCTCGCTTTCACCGGCGCAAGATGCAGGTCCCTACCGCTGAGGAGATGTCCAGCTTGACGC
  CAGAGTCCTCCCCGGAGCTGGCAAAACGCTCCTGGTTCGGGAACTTCATCTCCTTGGACAAAGAAGAACA
  AATATTCCTCGTGCTAAAGGACAAACCTCTCAGCAGCATCAAAGCAGACATCGTCCATGCCTTTCTGTCG
  ATCCCCAGCCTGAGTCACAGTGTGCTGTCACAGACCAGCTTCAGGGCCGAGTACAAGGCCAGTGGCGGCC
  CCTCCGTCTTCCAAAAGCCCGTCCGCTTCCAGGTGGACATCAGCTCCTCTGAGGGTCCAGAGCCCTCCCC
  GCGACGGGACGGCAGCGGAGGTGGTGGCATCTACTCCGTCACCTTCACTCTCATCTCGGGTCCCAGCCGT
  CGGTTCAAGCGAGTGGTGGAGACCATCCAGGCACAGCTCCTGAGCACTCATGACCAGCCCTCCGTGCAGG
  CCCTGGCAGACGAGAAGAACGGGGCCCAGACCCGGCCTGCTGGTGCCCCACCCCGAAGCCTGCAGCCCCC
  ACCCGGCCGCCCAGACCCAGAGCTGAGCAGCTCTCCCCGCCGAGGCCCCCCCAAGGACAAGAAGCTCCTG
  GCCACCAACGGGACCCCTCTGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211767 representing NM_032430
  Red=Cloning site Green=Tags(s)
  
  MSSGAKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAIKIVNREKL
  SESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQI
  VSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGR
  RADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQ
  KHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMI
  YYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGGGGSPVPTRRA
  LEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGE
  QPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSF
  LGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLS
  IPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSGGGGIYSVTFTLISGPSR
  RFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGAPPRSLQPPPGRPDPELSSSPRRGPPKDKKLL
  ATNGTPLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032430
• ORF Size: 2334 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032430.1, NP_115806.1
• RefSeq Size: 3109
• RefSeq ORF: 2337
• Locus ID: 84446
• Protein Families: Druggable Genome, Protein Kinase
• MW: 84.9 kDa