Rho GTPase activating protein 24 (ARHGAP24) (NM_001025616) Human Tagged ORF Clone

CAT#: RC211832

ARHGAP24 (Myc-DDK-tagged)-Human Rho GTPase activating protein 24 (ARHGAP24), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY422457) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211832 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ARHGAP24 protein (Cat# TP311832). The protein was produced from HEK293T cells transfected with ARHGAP24 cDNA clone (Cat# RC211832) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGAP24
• Synonyms: FILGAP; p73; p73RhoGAP; RC-GAP72; RCGAP72
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211832 representing NM_001025616
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGAACAATGACTCCACGGAGAACCCCCAACAAGGCCAAGGGCGGCAGAATGCCATCAAGTGTG
  GGTGGCTGAGGAAGCAAGGAGGCTTTGTCAAGACTTGGCATACTCGCTGGTTTGTGCTCAAGGGGGATCA
  GCTCTATTATTTCAAAGATGAAGATGAAACCAAGCCCTTGGGTACTATTTTTCTGCCTGGAAATAAAGTT
  TCTGAGCATCCCTGCAATGAAGAGAACCCAGGGAAGTTCCTTTTTGAAGTAGTTCCAGGAGGCGATCGAG
  ATCGGATGACAGCAAATCATGAAAGCTACCTCCTCATGGCAAGCACCCAGAATGATATGGAAGACTGGGT
  GAAGTCAATCCGCCGAGTCATATGGGGACCTTTCGGAGGAGGCATTTTTGGACAGAAACTGGAGGATACT
  GTTCGTTATGAGAAGAGATATGGGAACCGTCTGGCTCCGATGTTGGTGGAGCAGTGCGTGGACTTTATCC
  GACAAAGGGGGCTGAAAGAAGAGGGTCTCTTTCGACTGCCAGGCCAGGCTAATCTTGTTAAGGAGCTCCA
  AGATGCCTTTGACTGTGGGGAGAAGCCATCATTTGACAGCAACACAGATGTACACACGGTGGCATCACTT
  CTTAAGCTGTACCTCCGAGAACTTCCAGAACCAGTTATTCCTTATGCGAAGTATGAAGATTTTTTGTCAT
  GTGCCAAACTGCTCAGCAAGGAAGAGGAAGCAGGTGTTAAGGAATTAGCAAAGCAGGTGAAGAGTTTGCC
  AGTGGTAAATTACAACCTCCTCAAGTATATTTGCAGATTCTTGGATGAAGTACAGTCCTACTCGGGAGTT
  AACAAAATGAGTGTGCAGAACTTGGCAACGGTCTTTGGTCCTAATATCCTGCGCCCCAAAGTGGAAGATC
  CTTTGACTATCATGGAGGGCACTGTGGTGGTCCAGCAGTTGATGTCAGTGATGATTAGCAAACATGATTG
  CCTCTTTCCCAAAGATGCAGAACTACAAAGCAAGCCCCAAGATGGAGTGAGCAACAACAATGAAATTCAG
  AAGAAAGCCACCATGGGGCAGTTACAGAACAAGGAGAACAATAACACCAAGGACAGCCCTAGTAGGCAGT
  GCTCCTGGGACAAGTCTGAGTCACCCCAGAGAAGCAGCATGAACAATGGATCCCCCACAGCTCTATCAGG
  CAGCAAAACCAACAGCCCAAAGAACAGTGTTCACAAGCTAGATGTGTCTAGAAGCCCCCCTCTCATGGTC
  AAAAAGAACCCAGCCTTTAATAAGGGTAGTGGGATAGTTACCAATGGGTCCTTCAGCAGCAGTAATGCAG
  AAGGTCTTGAGAAAACCCAAACCACCCCCAATGGGAGCCTACAGGCCAGAAGGAGCTCTTCACTGAAGGT
  ATCTGGTACCAAAATGGGCACGCACAGTGTACAGAATGGAACGGTGCGCATGGGCATTTTGAACAGCGAC
  ACACTCGGGAACCCCACAAATGTTCGAAACATGAGCTGGCTGCCAAATGGCTATGTGACCCTGAGGGATA
  ACAAGCAGAAAGAACAAGCTGGAGAGTTAGGCCAGCACAACAGACTGTCCACCTATGATAATGTCCATCA
  ACAGTTCTCCATGATGAACCTTGATGACAAGCAGAGCATTGACAGTGCTACCTGGTCCACTTCCTCCTGT
  GAAATCTCCCTCCCTGAGAACTCCAACTCCTGTCGCTCTTCTACCACCACCTGCCCAGAGCAAGACTTTT
  TTGGGGGGAACTTTGAGGACCCTGTTTTGGATGGGCCCCCGCAGGACGACCTTTCCCACCCCAGGGACTA
  TGAAAGCAAAAGTGACCACAGGAGTGTGGGAGGTCGAAGTAGTCGTGCCACCAGTAGCAGTGACAACAGT
  GAGACATTTGTGGGCAACAGCAGCAGCAACCACAGTGCACTGCACAGTTTAGTTTCCAGCCTGAAACAGG
  AAATGACCAAACAGAAGATAGAGTATGAGTCCAGGATAAAGAGCTTAGAACAGCGAAACTTGACTTTGGA
  AACAGAAATGATGAGCCTCCATGATGAACTGGATCAGGAGAGGAAAAAGTTCACAATGATAGAAATAAAA
  ATGCGAAATGCCGAGCGAGCAAAAGAAGATGCCGAGAAAAGAAATGACATGCTACAGAAAGAAATGGAGC
  AGTTTTTTTCCACGTTTGGAGAACTGACAGTGGAACCCAGGAGAACCGAGAGAGGAAACACAATATGGAT
  TCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211832 representing NM_001025616
  Red=Cloning site Green=Tags(s)
  
  MEENNDSTENPQQGQGRQNAIKCGWLRKQGGFVKTWHTRWFVLKGDQLYYFKDEDETKPLGTIFLPGNKV
  SEHPCNEENPGKFLFEVVPGGDRDRMTANHESYLLMASTQNDMEDWVKSIRRVIWGPFGGGIFGQKLEDT
  VRYEKRYGNRLAPMLVEQCVDFIRQRGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASL
  LKLYLRELPEPVIPYAKYEDFLSCAKLLSKEEEAGVKELAKQVKSLPVVNYNLLKYICRFLDEVQSYSGV
  NKMSVQNLATVFGPNILRPKVEDPLTIMEGTVVVQQLMSVMISKHDCLFPKDAELQSKPQDGVSNNNEIQ
  KKATMGQLQNKENNNTKDSPSRQCSWDKSESPQRSSMNNGSPTALSGSKTNSPKNSVHKLDVSRSPPLMV
  KKNPAFNKGSGIVTNGSFSSSNAEGLEKTQTTPNGSLQARRSSSLKVSGTKMGTHSVQNGTVRMGILNSD
  TLGNPTNVRNMSWLPNGYVTLRDNKQKEQAGELGQHNRLSTYDNVHQQFSMMNLDDKQSIDSATWSTSSC
  EISLPENSNSCRSSTTTCPEQDFFGGNFEDPVLDGPPQDDLSHPRDYESKSDHRSVGGRSSRATSSSDNS
  ETFVGNSSSNHSALHSLVSSLKQEMTKQKIEYESRIKSLEQRNLTLETEMMSLHDELDQERKKFTMIEIK
  MRNAERAKEDAEKRNDMLQKEMEQFFSTFGELTVEPRRTERGNTIWIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025616
• ORF Size: 2244 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025616.1, NM_001025616.2, NP_001020787.1, NP_001020787.2
• RefSeq Size: 2936
• RefSeq ORF: 2247
• Locus ID: 83478
• MW: 84.1 kDa
• Gene Summary: This gene encodes a Rho-GTPase activating protein, which is specific for the small GTPase family member Rac. Binding of the encoded protein by filamin A targets it to sites of membrane protrusion, where it antognizes Rac. This results in suppression of lamellae formation and promotion of retraction to regulate cell polarity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]