Calpastatin (CAST) (NM_001042440) Human Tagged ORF Clone

CAT#: RC211937

CAST (Myc-DDK-tagged)-Human calpastatin (CAST), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CAST
• Synonyms: BS-17; PLACK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211937 representing NM_001042440
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCAGCCCGGCCAGAAGCCCGCCGCCTCCCCGCGGCCCCGGCGAGCAGCCGCCGCCCGCCGCACCC
  ATGAGCATGTCAGTGAAAAAACCAGTGAATCGCCTTCCAAACCAGGAGAAAAGAAAGGATCAGATGAGAA
  AAAAGCAGCAAGCCTCGGCAGCAGTCAATCCTCCAGAACCTATGCTGGTGGAACAGCCTCGGCCACCAAG
  GTGTCAGCTTCCTCTGGTGCAACCAGCAAGTCTTCCAGTATGAATCCCACAGAAACCAAGGCTGTAAAAA
  CAGAACCTGAGAAGAAGTCACAGTCAACCAAGCCAAAAAGCCTACCCAAGCAGGCATCAGATACAGGAAG
  TAACGATGCTCACAATAAAAAAGCAGTTTCCAGATCAGCTGAACAGCAGCCATCAGAGAAATCAACAGAA
  CCAAAGACTAAACCACAAGACATGATTTCTGCTGGTGGAGAGAGTGTTGCTGGTATCACTGCAATATCTG
  GCAAGCCGGGTGACAAGAAAAAAGAAAAGAAATCATTAACCCCAGCTGTGCCAGTTGAATCTAAACCGGA
  TAAACCATCGGGAAAGTCAGGCATGGATGCTGCTTTGGATGACTTAATAGATACTTTAGGAGGACCTGAA
  GAAACTGAAGAAGAAAATACAACGTATACTGGACCAGAAGTTTCAGATCCAATGAGTTCCACCTACATAG
  AGGAATTGGGTAAAAGAGAAGTCACAATTCCTCCAAAATATAGGGAACTATTGGCTAAAAAGGAAGGGAT
  CACAGGGCCTCCTGCAGACTCTTCGAAACCCATAGGGCCAGATGATGCTATAGACGCCTTGTCATCTGAC
  TTCACCTGTGGGTCGCCTACAGCTGCTGGAAAGAAAACTGAAAAAGAGGAATCTACAGAAGTTTTAAAAG
  CTCAGTCAGCAGGGACAGTCAGAAGTGCTGCTCCACCCCAAGAGAAGAAAAGAAAGGTGGAGAAGGATAC
  AATGAGTGATCAAGCACTCGAGGCTCTGTCGGCTTCACTGGGCACCCGGCAAGCAGAACCTGAGCTCGAC
  CTCCGCTCAATTAAGGAAGTCGATGAGGCAAAAGCTAAAGAAGAAAAACTAGAGAAGTGTGGTGAGGATG
  ATGAAACAATCCCATCTGAGTACAGATTAAAACCAGCCACGGATAAAGATGGAAAACCACTATTGCCAGA
  GCCTGAAGAAAAACCCAAGCCTCGGAGTGAATCAGAACTCATTGATGAACTTTCAGAAGATTTTGACCGG
  TCTGAATGTAAAGAGAAACCATCTAAGCCAACTGAAAAGACAGAAGAATCTAAGGCCGCTGCTCCAGCTC
  CTGTGTCGGAGGCTGTGTGTCGGACCTCCATGTGTAGTATACAGTCAGCACCCCCTGAGCCGGCTACCTT
  GAAGGGCACAGTGCCAGATGATGCTGTAGAAGCCTTGGCTGATAGCCTGGGGAAAAAGGAAGCAGATCCA
  GAAGATGGAAAACCTGTGATGGATAAAGTCAAGGAGAAGGCCAAAGAAGAAGACCGTGAAAAGCTTGGTG
  AAAAAGAAGAAACAATTCCTCCTGATTATAGATTAGAAGAGGTCAAGGATAAAGATGGAAAGCCACTCCT
  GCCAAAAGAGTCTAAGGAACAGCTTCCACCCATGAGTGAAGACTTCCTTCTGGATGCTTTGTCTGAGGAC
  TTCTCTGGTCCACAAAATGCTTCATCTCTTAAATTTGAAGATGCTAAACTTGCTGCTGCCATCTCTGAAG
  TGGTTTCCCAAACCCCAGCTTCAACGACCCAAGCTGGAGCCCCACCCCGTGATACCTCGCAGAGTGACAA
  AGACCTCGATGATGCCTTGGATAAACTCTCTGACAGTCTAGGACAAAGGCAGCCTGACCCAGATGAGAAC
  AAACCAATGGAAGATAAAGTAAAGGAAAAAGCTAAAGCTGAACATAGAGACAAGCTTGGAGAAAGAGATG
  ACACTATCCCACCTGAATACAGACATCTCCTGGATGATAATGGACAGGACAAACCAGTGAAGCCACCTAC
  AAAGAAATCAGAGGATTCAAAGAAACCTGCAGATGACCAAGACCCCATTGATGCTCTCTCAGGAGATCTG
  GACAGCTGTCCCTCCACTACAGAAACCTCACAGAACACAGCAAAGGATAAGTGCAAGAAGGCTGCTTCCA
  GCTCCAAAGCACCTAAGAATGGAGGTAAAGCGAAGGATTCAGCAAAGACAACAGAGGAAACTTCCAAGCC
  AAAAGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211937 representing NM_001042440
  Red=Cloning site Green=Tags(s)
  
  MSQPGQKPAASPRPRRAAAARRTHEHVSEKTSESPSKPGEKKGSDEKKAASLGSSQSSRTYAGGTASATK
  VSASSGATSKSSSMNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTE
  PKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPE
  ETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSD
  FTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELD
  LRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDR
  SECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADP
  EDGKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSED
  FSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDEN
  KPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDL
  DSCPSTTETSQNTAKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042440
• ORF Size: 2250 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042440.1, NM_001042440.2, NM_001042440.3, NM_001042440.4, NP_001035905.1
• RefSeq Size: 4550 bp
• RefSeq ORF: 2253 bp
• Locus ID: 831
• Cytogenetics: 5q15
• MW: 80.3 kDa
• Gene Summary: 'The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]'