AFM (NM_001133) Human Tagged ORF Clone
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | AFM |
Synonyms | ALB2; ALBA; ALF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC211969 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACTACTAAAACTTACAGGTTTTATTTTTTTCTTGTTTTTTTTGACTGAATCCCTAACCCTGCCCA CACAACCTCGGGATATAGAGAACTTCAATAGTACTCAAAAATTTATAGAAGATAATATTGAATACATCAC CATCATTGCATTTGCTCAGTATGTTCAGGAAGCAACCTTTGAAGAAATGGAAAAGCTGGTGAAAGACATG GTAGAATACAAAGACAGATGTATGGCTGACAAGACGCTCCCAGAGTGTTCAAAATTACCTAATAATGTTT TACAGGAAAAAATATGTGCTATGGAGGGGCTGCCACAAAAGCATAATTTCTCACACTGCTGCAGTAAGGT TGATGCTCAAAGAAGACTCTGTTTCTTCTATAACAAGAAATCTGATGTGGGATTTCTGCCTCCTTTCCCT ACCCTGGATCCCGAAGAGAAATGCCAGGCTTATGAAAGTAACAGAGAATCCCTTTTAAATCACTTTTTAT ATGAAGTTGCCAGAAGGAACCCATTTGTCTTCGCCCCTACACTTCTAACTGTTGCTGTTCATTTTGAGGA GGTGGCCAAATCATGTTGTGAAGAACAAAACAAAGTCAACTGCCTTCAAACAAGGGCAATACCTGTCACA CAATATTTAAAAGCATTTTCTTCTTATCAAAAACATGTCTGTGGGGCACTTTTGAAATTTGGAACCAAAG TTGTACACTTTATATATATTGCGATACTCAGTCAAAAATTCCCCAAGATTGAATTTAAGGAGCTTATTTC TCTTGTAGAAGATGTTTCTTCCAACTATGATGGATGCTGTGAAGGGGATGTTGTGCAGTGCATCCGTGAC ACGAGCAAGGTTATGAACCATATTTGTTCAAAACAAGATTCTATCTCCAGCAAAATCAAAGAGTGCTGTG AAAAGAAAATACCAGAGCGCGGCCAGTGCATAATTAACTCAAACAAAGATGATAGACCAAAGGATTTATC TCTAAGAGAAGGAAAATTTACTGACAGTGAAAATGTGTGTCAAGAACGAGATGCTGACCCAGACACCTTC TTTGCGAAGTTTACTTTTGAATACTCAAGGAGACATCCAGACCTGTCTATACCAGAGCTTTTAAGAATTG TTCAAATATACAAAGATCTCCTGAGAAATTGCTGCAACACAGAAAACCCTCCAGGTTGTTACCGTTACGC GGAAGACAAATTCAATGAGACAACTGAGAAAAGCCTCAAGATGGTACAACAAGAATGTAAACATTTCCAG AATTTGGGGAAGGATGGTTTGAAATACCATTACCTCATCAGGCTCACGAAGATAGCTCCCCAACTCTCCA CTGAAGAACTGGTGTCTCTTGGCGAGAAAATGGTGACAGCTTTCACTACTTGCTGTACGCTAAGTGAAGA GTTTGCCTGTGTTGATAATTTGGCAGATTTAGTTTTTGGAGAGTTATGTGGAGTAAATGAAAATCGAACT ATCAACCCTGCTGTGGACCACTGCTGTAAAACAAACTTTGCCTTCAGAAGGCCCTGCTTTGAGAGTTTGA AAGCTGATAAAACATATGTGCCTCCACCTTTCTCTCAAGATTTATTTACCTTTCACGCAGACATGTGTCA ATCTCAGAATGAGGAGCTTCAGAGGAAGACAGACAGGTTTCTTGTCAACTTAGTGAAGCTGAAGCATGAA CTCACAGATGAAGAGCTGCAGTCTTTGTTTACAAATTTCGCAAATGTAGTGGATAAGTGCTGCAAAGCAG AGAGTCCTGAAGTCTGCTTTAATGAAGAGAGTCCAAAAATTGGCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211969 protein sequence
Red=Cloning site Green=Tags(s) MKLLKLTGFIFFLFFLTESLTLPTQPRDIENFNSTQKFIEDNIEYITIIAFAQYVQEATFEEMEKLVKDM VEYKDRCMADKTLPECSKLPNNVLQEKICAMEGLPQKHNFSHCCSKVDAQRRLCFFYNKKSDVGFLPPFP TLDPEEKCQAYESNRESLLNHFLYEVARRNPFVFAPTLLTVAVHFEEVAKSCCEEQNKVNCLQTRAIPVT QYLKAFSSYQKHVCGALLKFGTKVVHFIYIAILSQKFPKIEFKELISLVEDVSSNYDGCCEGDVVQCIRD TSKVMNHICSKQDSISSKIKECCEKKIPERGQCIINSNKDDRPKDLSLREGKFTDSENVCQERDADPDTF FAKFTFEYSRRHPDLSIPELLRIVQIYKDLLRNCCNTENPPGCYRYAEDKFNETTEKSLKMVQQECKHFQ NLGKDGLKYHYLIRLTKIAPQLSTEELVSLGEKMVTAFTTCCTLSEEFACVDNLADLVFGELCGVNENRT INPAVDHCCKTNFAFRRPCFESLKADKTYVPPPFSQDLFTFHADMCQSQNEELQRKTDRFLVNLVKLKHE LTDEELQSLFTNFANVVDKCCKAESPEVCFNEESPKIGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001133 |
ORF Size | 1797 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001133.1, NM_001133.2, NP_001124.1 |
RefSeq Size | 1997 bp |
RefSeq ORF | 1800 bp |
Locus ID | 173 |
Cytogenetics | 4q13.3 |
Domains | ALBUMIN |
Protein Families | Druggable Genome, Secreted Protein |
MW | 69.1 kDa |
Gene Summary | 'This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC119416 | AFM (untagged)-Human afamin (AFM) |
USD 760.00 |
|
RG211969 | AFM (GFP-tagged) - Human afamin (AFM) |
USD 460.00 |
|
RC211969L1 | Lenti ORF clone of Human afamin (AFM), Myc-DDK-tagged |
USD 620.00 |
|
RC211969L2 | Lenti ORF clone of Human afamin (AFM), mGFP tagged |
USD 620.00 |
|
RC211969L3 | Lenti ORF clone of Human afamin (AFM), Myc-DDK-tagged |
USD 620.00 |
|
RC211969L4 | Lenti ORF clone of Human afamin (AFM), mGFP tagged |
USD 620.00 |
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