AFM (NM_001133) Human Tagged ORF Clone

CAT#: RC211969

AFM (Myc-DDK-tagged)-Human afamin (AFM)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AFM
• Synonyms: ALB2; ALBA; ALF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211969 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACTACTAAAACTTACAGGTTTTATTTTTTTCTTGTTTTTTTTGACTGAATCCCTAACCCTGCCCA
  CACAACCTCGGGATATAGAGAACTTCAATAGTACTCAAAAATTTATAGAAGATAATATTGAATACATCAC
  CATCATTGCATTTGCTCAGTATGTTCAGGAAGCAACCTTTGAAGAAATGGAAAAGCTGGTGAAAGACATG
  GTAGAATACAAAGACAGATGTATGGCTGACAAGACGCTCCCAGAGTGTTCAAAATTACCTAATAATGTTT
  TACAGGAAAAAATATGTGCTATGGAGGGGCTGCCACAAAAGCATAATTTCTCACACTGCTGCAGTAAGGT
  TGATGCTCAAAGAAGACTCTGTTTCTTCTATAACAAGAAATCTGATGTGGGATTTCTGCCTCCTTTCCCT
  ACCCTGGATCCCGAAGAGAAATGCCAGGCTTATGAAAGTAACAGAGAATCCCTTTTAAATCACTTTTTAT
  ATGAAGTTGCCAGAAGGAACCCATTTGTCTTCGCCCCTACACTTCTAACTGTTGCTGTTCATTTTGAGGA
  GGTGGCCAAATCATGTTGTGAAGAACAAAACAAAGTCAACTGCCTTCAAACAAGGGCAATACCTGTCACA
  CAATATTTAAAAGCATTTTCTTCTTATCAAAAACATGTCTGTGGGGCACTTTTGAAATTTGGAACCAAAG
  TTGTACACTTTATATATATTGCGATACTCAGTCAAAAATTCCCCAAGATTGAATTTAAGGAGCTTATTTC
  TCTTGTAGAAGATGTTTCTTCCAACTATGATGGATGCTGTGAAGGGGATGTTGTGCAGTGCATCCGTGAC
  ACGAGCAAGGTTATGAACCATATTTGTTCAAAACAAGATTCTATCTCCAGCAAAATCAAAGAGTGCTGTG
  AAAAGAAAATACCAGAGCGCGGCCAGTGCATAATTAACTCAAACAAAGATGATAGACCAAAGGATTTATC
  TCTAAGAGAAGGAAAATTTACTGACAGTGAAAATGTGTGTCAAGAACGAGATGCTGACCCAGACACCTTC
  TTTGCGAAGTTTACTTTTGAATACTCAAGGAGACATCCAGACCTGTCTATACCAGAGCTTTTAAGAATTG
  TTCAAATATACAAAGATCTCCTGAGAAATTGCTGCAACACAGAAAACCCTCCAGGTTGTTACCGTTACGC
  GGAAGACAAATTCAATGAGACAACTGAGAAAAGCCTCAAGATGGTACAACAAGAATGTAAACATTTCCAG
  AATTTGGGGAAGGATGGTTTGAAATACCATTACCTCATCAGGCTCACGAAGATAGCTCCCCAACTCTCCA
  CTGAAGAACTGGTGTCTCTTGGCGAGAAAATGGTGACAGCTTTCACTACTTGCTGTACGCTAAGTGAAGA
  GTTTGCCTGTGTTGATAATTTGGCAGATTTAGTTTTTGGAGAGTTATGTGGAGTAAATGAAAATCGAACT
  ATCAACCCTGCTGTGGACCACTGCTGTAAAACAAACTTTGCCTTCAGAAGGCCCTGCTTTGAGAGTTTGA
  AAGCTGATAAAACATATGTGCCTCCACCTTTCTCTCAAGATTTATTTACCTTTCACGCAGACATGTGTCA
  ATCTCAGAATGAGGAGCTTCAGAGGAAGACAGACAGGTTTCTTGTCAACTTAGTGAAGCTGAAGCATGAA
  CTCACAGATGAAGAGCTGCAGTCTTTGTTTACAAATTTCGCAAATGTAGTGGATAAGTGCTGCAAAGCAG
  AGAGTCCTGAAGTCTGCTTTAATGAAGAGAGTCCAAAAATTGGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211969 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKLLKLTGFIFFLFFLTESLTLPTQPRDIENFNSTQKFIEDNIEYITIIAFAQYVQEATFEEMEKLVKDM
  VEYKDRCMADKTLPECSKLPNNVLQEKICAMEGLPQKHNFSHCCSKVDAQRRLCFFYNKKSDVGFLPPFP
  TLDPEEKCQAYESNRESLLNHFLYEVARRNPFVFAPTLLTVAVHFEEVAKSCCEEQNKVNCLQTRAIPVT
  QYLKAFSSYQKHVCGALLKFGTKVVHFIYIAILSQKFPKIEFKELISLVEDVSSNYDGCCEGDVVQCIRD
  TSKVMNHICSKQDSISSKIKECCEKKIPERGQCIINSNKDDRPKDLSLREGKFTDSENVCQERDADPDTF
  FAKFTFEYSRRHPDLSIPELLRIVQIYKDLLRNCCNTENPPGCYRYAEDKFNETTEKSLKMVQQECKHFQ
  NLGKDGLKYHYLIRLTKIAPQLSTEELVSLGEKMVTAFTTCCTLSEEFACVDNLADLVFGELCGVNENRT
  INPAVDHCCKTNFAFRRPCFESLKADKTYVPPPFSQDLFTFHADMCQSQNEELQRKTDRFLVNLVKLKHE
  LTDEELQSLFTNFANVVDKCCKAESPEVCFNEESPKIGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001133
• ORF Size: 1797 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001133.1, NM_001133.2, NP_001124.1
• RefSeq Size: 1997 bp
• RefSeq ORF: 1800 bp
• Locus ID: 173
• Cytogenetics: 4q13.3
• Domains: ALBUMIN
• Protein Families: Druggable Genome, Secreted Protein
• MW: 69.1 kDa
• Gene Summary: 'This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. [provided by RefSeq, Jul 2008]'