PDE11A (NM_001077358) Human Tagged ORF Clone

CAT#: RC211991

PDE11A (Myc-DDK-tagged)-Human phosphodiesterase 11A (PDE11A), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDE11A
• Synonyms: PPNAD2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211991 representing NM_001077358
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGATGTATCTTCCATTTTGTGGAATCGCCATATCTAACGCTCAGCTCTTTGCTGCCTCAAGGAAAG
  AATATGAAAGAAGCAGAGCTTTGCTAGAGGTGGTTAATGACCTCTTTGAAGAACAGACTGACCTGGAGAA
  AATTGTCAAGAAAATAATGCATCGGGCCCAAACTCTGCTGAAATGTGAACGCTGTTCTGTTTTACTCCTA
  GAGGACATCGAATCACCAGTGGTGAAATTTACCAAATCCTTTGAATTGATGTCCCCAAAGTGCAGTGCTG
  ATGCTGAGAACAGTTTCAAAGAAAGCATGGAGAAATCATCATACTCCGACTGGCTAATAAATAACAGCAT
  TGCTGAGCTGGTTGCTTCAACAGGCCTTCCAGTGAACATCAGTGATGCCTACCAGGATCCGCGCTTTGAT
  GCAGAGGCAGACCAGATATCTGGTTTTCACATAAGATCTGTTCTTTGTGTCCCTATTTGGAATAGCAACC
  ACCAAATAATTGGAGTGGCTCAAGTGTTAAACAGACTTGATGGGAAACCTTTTGATGATGCAGATCAACG
  ACTTTTTGAGGCTTTTGTCATCTTTTGTGGACTTGGCATCAACAACACAATTATGTATGATCAAGTGAAG
  AAGTCCTGGGCCAAGCAGTCTGTGGCTCTTGATGTGCTATCATACCATGCAACATGTTCAAAAGCTGAAG
  TTGACAAGTTTAAGGCAGCCAACATCCCTCTGGTGTCAGAACTTGCCATCGATGACATTCATTTTGATGA
  CTTTTCTCTCGACGTTGATGCCATGATCACAGCTGCTCTCCGGATGTTCATGGAGCTGGGGATGGTACAG
  AAATTTAAAATTGACTATGAGACACTGTGTAGGTGGCTTTTGACAGTGAGGAAAAACTATCGGATGGTTC
  TATACCACAACTGGAGACATGCCTTCAACGTGTGTCAGCTGATGTTCGCGATGTTAACCACTGCTGGGTT
  TCAAGACATTCTGACCGAGGTGGAAATTTTAGCGGTGATTGTGGGATGCCTGTGTCATGACCTCGACCAC
  AGGGGAACCAACAATGCCTTCCAAGCTAAGAGTGGCTCTGCCCTGGCCCAACTCTATGGAACCTCTGCTA
  CCTTGGAGCATCACCATTTCAACCACGCCGTGATGATCCTTCAAAGTGAGGGTCACAATATCTTTGCTAA
  CCTGTCCTCCAAGGAATATAGTGACCTTATGCAGCTTTTGAAGCAGTCAATATTGGCAACAGACCTCACG
  CTGTACTTTGAGAGGAGAACTGAATTCTTTGAACTTGTCAGTAAAGGAGAATACGATTGGAACATCAAAA
  ACCATCGTGATATATTTCGATCAATGTTAATGACAGCCTGTGACCTTGGAGCCGTGACCAAACCGTGGGA
  GATCTCCAGACAGGTGGCAGAACTTGTAACCAGTGAGTTCTTCGAACAAGGAGATCGGGAGAGATTAGAG
  CTCAAACTCACTCCTTCAGCAATTTTTGATCGGAACCGGAAGGATGAACTGCCTCGGTTGCAACTGGAGT
  GGATTGATAGCATCTGCATGCCTTTGTATCAGGCACTGGTGAAGGTCAACGTGAAACTGAAGCCGATGCT
  AGATTCAGTAGCTACAAACAGAAGTAAGTGGGAAGAGCTACACCAAAAACGACTGCTGGCCTCAACTGCC
  TCATCCTCCCCTGCCAGTGTTATGGTAGCCAAGGAAGACAGGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211991 representing NM_001077358
  Red=Cloning site Green=Tags(s)
  
  MQMYLPFCGIAISNAQLFAASRKEYERSRALLEVVNDLFEEQTDLEKIVKKIMHRAQTLLKCERCSVLLL
  EDIESPVVKFTKSFELMSPKCSADAENSFKESMEKSSYSDWLINNSIAELVASTGLPVNISDAYQDPRFD
  AEADQISGFHIRSVLCVPIWNSNHQIIGVAQVLNRLDGKPFDDADQRLFEAFVIFCGLGINNTIMYDQVK
  KSWAKQSVALDVLSYHATCSKAEVDKFKAANIPLVSELAIDDIHFDDFSLDVDAMITAALRMFMELGMVQ
  KFKIDYETLCRWLLTVRKNYRMVLYHNWRHAFNVCQLMFAMLTTAGFQDILTEVEILAVIVGCLCHDLDH
  RGTNNAFQAKSGSALAQLYGTSATLEHHHFNHAVMILQSEGHNIFANLSSKEYSDLMQLLKQSILATDLT
  LYFERRTEFFELVSKGEYDWNIKNHRDIFRSMLMTACDLGAVTKPWEISRQVAELVTSEFFEQGDRERLE
  LKLTPSAIFDRNRKDELPRLQLEWIDSICMPLYQALVKVNVKLKPMLDSVATNRSKWEELHQKRLLASTA
  SSSPASVMVAKEDRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077358
• ORF Size: 1725 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001077358.1, NP_001070826.1
• RefSeq Size: 1869
• RefSeq ORF: 1728
• Locus ID: 50940
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• MW: 65.68 kDa
• Gene Summary: The 3',5'-cyclic nucleotides cAMP and cGMP function as second messengers in a wide variety of signal transduction pathways. 3',5'-cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and cGMP to the corresponding 5'-monophosphates and provide a mechanism to downregulate cAMP and cGMP signaling. This gene encodes a member of the PDE protein superfamily. Mutations in this gene are a cause of Cushing disease and adrenocortical hyperplasia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]