PIGR (NM_002644) Human Tagged ORF Clone

CAT#: RC212006

PIGR (Myc-DDK-tagged)-Human polymeric immunoglobulin receptor (PIGR)

Product Images

Western blot validation of overexpression lysate (Cat# LY400939) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212006 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PIGR protein (Cat# TP312006). The protein was produced from HEK293T cells transfected with PIGR cDNA clone (Cat# RC212006) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIGR
• Synonyms: FLJ22667; MGC125361; MGC125362
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212006 representing NM_002644
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTCTTCGTGCTCACCTGCCTGCTGGCGGTCTTCCCAGCCATCTCCACGAAGAGTCCCATATTTG
  GTCCCGAGGAGGTGAATAGTGTGGAAGGTAACTCAGTGTCCATCACGTGCTACTACCCACCCACCTCTGT
  CAACCGGCACACCCGGAAGTACTGGTGCCGGCAGGGAGCTAGAGGTGGCTGCATAACCCTCATCTCCTCG
  GAGGGCTACGTCTCCAGCAAATATGCAGGCAGGGCTAACCTCACCAACTTCCCGGAGAACGGCACATTTG
  TGGTGAACATTGCCCAGCTGAGCCAGGATGACTCCGGGCGCTACAAGTGTGGCCTGGGCATCAATAGCCG
  AGGCCTGTCCTTTGATGTCAGCCTGGAGGTCAGCCAGGGTCCTGGGCTCCTAAATGACACTAAAGTCTAC
  ACAGTGGACCTGGGCAGAACGGTGACCATCAACTGCCCTTTCAAGACTGAGAATGCTCAAAAGAGGAAGT
  CCTTGTACAAGCAGATAGGCCTGTACCCTGTGCTGGTCATCGACTCCAGTGGTTATGTAAATCCCAACTA
  TACAGGAAGAATACGCCTTGATATTCAGGGTACTGGCCAGTTACTGTTCAGCGTTGTCATCAACCAACTC
  AGGCTCAGCGATGCTGGGCAGTATCTCTGCCAGGCTGGGGATGATTCCAATAGTAATAAGAAGAATGCTG
  ACCTCCAAGTGCTAAAGCCCGAGCCCGAGCTGGTTTATGAAGACCTGAGGGGCTCAGTGACCTTCCACTG
  TGCCCTGGGCCCTGAGGTGGCAAACGTGGCCAAATTTCTGTGCCGACAGAGCAGTGGGGAAAACTGTGAC
  GTGGTCGTCAACACCCTGGGGAAGAGGGCCCCAGCCTTTGAGGGCAGGATCCTGCTCAACCCCCAGGACA
  AGGATGGCTCATTCAGTGTGGTGATCACAGGCCTGAGGAAGGAGGATGCAGGGCGCTACCTGTGTGGAGC
  CCATTCGGATGGTCAGCTGCAGGAAGGCTCGCCTATCCAGGCCTGGCAACTCTTCGTCAATGAGGAGTCC
  ACGATTCCCCGCAGCCCCACTGTGGTGAAGGGGGTGGCAGGAGGCTCTGTGGCCGTGCTCTGCCCCTACA
  ACCGTAAGGAAAGCAAAAGCATCAAGTACTGGTGTCTCTGGGAAGGGGCCCAGAATGGCCGCTGCCCCCT
  GCTGGTGGACAGCGAGGGGTGGGTTAAGGCCCAGTACGAGGGCCGCCTCTCCCTGCTGGAGGAGCCAGGC
  AACGGCACCTTCACTGTCATCCTCAACCAGCTCACCAGCCGGGACGCCGGCTTCTACTGGTGTCTGACCA
  ACGGCGATACTCTCTGGAGGACCACCGTGGAGATCAAGATTATCGAAGGAGAACCAAACCTCAAGGTACC
  AGGGAATGTCACGGCTGTGCTGGGAGAGACTCTCAAGGTCCCCTGTCACTTTCCATGCAAATTCTCCTCG
  TACGAGAAATACTGGTGCAAGTGGAATAACACGGGCTGCCAGGCCCTGCCCAGCCAAGACGAAGGCCCCA
  GCAAGGCCTTCGTGAACTGTGACGAGAACAGCCGGCTTGTCTCCCTGACCCTGAACCTGGTGACCAGGGC
  TGATGAGGGCTGGTACTGGTGTGGAGTGAAGCAGGGCCACTTCTATGGAGAGACTGCAGCCGTCTATGTG
  GCAGTTGAAGAGAGGAAGGCAGCGGGGTCCCGCGATGTCAGCCTAGCGAAGGCAGACGCTGCTCCTGATG
  AGAAGGTGCTAGACTCTGGTTTTCGGGAGATTGAGAACAAAGCCATTCAGGATCCCAGGCTTTTTGCAGA
  GGAAAAGGCGGTGGCAGATACAAGAGATCAAGCCGATGGGAGCAGAGCATCTGTGGATTCCGGCAGCTCT
  GAGGAACAAGGTGGAAGCTCCAGAGCGCTGGTCTCCACCCTGGTGCCCCTGGGCCTGGTGCTGGCAGTGG
  GAGCCGTGGCTGTGGGGGTGGCCAGAGCCCGGCACAGGAAGAACGTCGACCGAGTTTCAATCAGAAGCTA
  CAGGACAGACATTAGCATGTCAGACTTCGAGAACTCCAGGGAATTTGGAGCCAATGACAACATGGGAGCC
  TCTTCGATCACTCAGGAGACATCCCTCGGAGGAAAAGAAGAGTTTGTTGCCACCACTGAGAGCACCACAG
  AGACCAAAGAACCCAAGAAGGCAAAAAGGTCATCCAAGGAGGAAGCCGAGATGGCCTACAAAGACTTCCT
  GCTCCAGTCCAGCACCGTGGCCGCCGAGGCCCAGGACGGCCCCCAGGAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212006 representing NM_002644
  Red=Cloning site Green=Tags(s)
  
  MLLFVLTCLLAVFPAISTKSPIFGPEEVNSVEGNSVSITCYYPPTSVNRHTRKYWCRQGARGGCITLISS
  EGYVSSKYAGRANLTNFPENGTFVVNIAQLSQDDSGRYKCGLGINSRGLSFDVSLEVSQGPGLLNDTKVY
  TVDLGRTVTINCPFKTENAQKRKSLYKQIGLYPVLVIDSSGYVNPNYTGRIRLDIQGTGQLLFSVVINQL
  RLSDAGQYLCQAGDDSNSNKKNADLQVLKPEPELVYEDLRGSVTFHCALGPEVANVAKFLCRQSSGENCD
  VVVNTLGKRAPAFEGRILLNPQDKDGSFSVVITGLRKEDAGRYLCGAHSDGQLQEGSPIQAWQLFVNEES
  TIPRSPTVVKGVAGGSVAVLCPYNRKESKSIKYWCLWEGAQNGRCPLLVDSEGWVKAQYEGRLSLLEEPG
  NGTFTVILNQLTSRDAGFYWCLTNGDTLWRTTVEIKIIEGEPNLKVPGNVTAVLGETLKVPCHFPCKFSS
  YEKYWCKWNNTGCQALPSQDEGPSKAFVNCDENSRLVSLTLNLVTRADEGWYWCGVKQGHFYGETAAVYV
  AVEERKAAGSRDVSLAKADAAPDEKVLDSGFREIENKAIQDPRLFAEEKAVADTRDQADGSRASVDSGSS
  EEQGGSSRALVSTLVPLGLVLAVGAVAVGVARARHRKNVDRVSIRSYRTDISMSDFENSREFGANDNMGA
  SSITQETSLGGKEEFVATTESTTETKEPKKAKRSSKEEAEMAYKDFLLQSSTVAAEAQDGPQEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002644
• ORF Size: 2292 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002644.1, NM_002644.2, NM_002644.3, NP_002635.2
• RefSeq Size: 4266 bp
• RefSeq ORF: 2295 bp
• Locus ID: 5284
• Cytogenetics: 1q32.1
• Domains: ig, IGv, IG
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• MW: 83.28 kDa
• Gene Summary: 'This gene is a member of the immunoglobulin superfamily. The encoded poly-Ig receptor binds polymeric immunoglobulin molecules at the basolateral surface of epithelial cells; the complex is then transported across the cell to be secreted at the apical surface. A significant association was found between immunoglobulin A nephropathy and several SNPs in this gene.[provided by RefSeq, Sep 2009]'