ZDHHC22 (NM_174976) Human Tagged ORF Clone

CAT#: RC212063

ZDHHC22 (Myc-DDK-tagged)-Human zinc finger, DHHC-type containing 22 (ZDHHC22)

Product Images

Western blot validation of overexpression lysate (Cat# LY406367) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212063 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZDHHC22
• Synonyms: C14orf59
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212063 representing NM_174976
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGCCCTGCGGCTGCTCAACGTGGTGGCCCCCGCCTACTTCTTGTGCATCTCCCTGGTGACCTTCG
  TGCTGCAGCTCTTCCTCTTCCTGCCCAGCATGCGCGAGGACCCCGCGGCCGCCCGGCTCTTCTCGCCCGC
  CCTGCTCCACGGGGCGCTCTTCCTATTCCTCTCGGCCAACGCCCTGGGCAATTACGTCCTTGTCATCCAG
  AACTCCCCAGACGACCTGGGGGCCTGCCAGGGGGCCTCGGCCAGGAAGACTCCATGCCCCTCACCTAGCA
  CCCACTTCTGCCGAGTGTGCGCCAGAGTCACCCTGAGGCACGACCATCACTGTTTCTTCACCGGCAACTG
  CATCGGCAGCAGGAACATGCGCAACTTCGTCCTGTTCTGCCTCTACACCTCCCTGGCCTGCCTCTACTCC
  ATGGTGGCCGGCGTGGCCTACATCTCCGCTGTCCTTTCCATCTCCTTCGCCCACCCCTTGGCCTTCCTCA
  CGCTCCTGCCCACCTCCATCAGCCAGTTCTTCTCCGGAGCTGTCCTGGGTTCTGAAATGTTCGTCATCCT
  CATGCTCTACCTCTGGTTCGCCATCGGCCTGGCCTGCGCCGGCTTCTGCTGCCACCAGCTGCTGTTGATC
  CTCCGCGGGCAGACCCGCCACCAGGTGCGGAAGGGGGTGGCAGTGAGGGCCCGGCCCTGGCGCAAGAACT
  TACAAGAGGTCTTCGGAAAGAGGTGGCTGCTGGGCCTGCTGGTCCCCATGTTCAATGTCGGAAGTGAGAG
  CTCCAAGCAGCAGGATAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212063 representing NM_174976
  Red=Cloning site Green=Tags(s)
  
  MLALRLLNVVAPAYFLCISLVTFVLQLFLFLPSMREDPAAARLFSPALLHGALFLFLSANALGNYVLVIQ
  NSPDDLGACQGASARKTPCPSPSTHFCRVCARVTLRHDHHCFFTGNCIGSRNMRNFVLFCLYTSLACLYS
  MVAGVAYISAVLSISFAHPLAFLTLLPTSISQFFSGAVLGSEMFVILMLYLWFAIGLACAGFCCHQLLLI
  LRGQTRHQVRKGVAVRARPWRKNLQEVFGKRWLLGLLVPMFNVGSESSKQQDK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_174976
• ORF Size: 789 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_174976.2, NP_777636.2
• RefSeq Size: 3408
• RefSeq ORF: 792
• Locus ID: 283576
• MW: 28.9 kDa