VANGL2 (NM_020335) Human Tagged ORF Clone

CAT#: RC212173

VANGL2 (Myc-DDK-tagged)-Human vang-like 2 (van gogh, Drosophila) (VANGL2)

Product Images

Western blot validation of overexpression lysate (Cat# LY412565) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212173 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VANGL2
• Synonyms: LPP1; LTAP; STB1; STBM; STBM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212173 representing NM_020335
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACCGAGTCCCAGTACTCGGGCTATTCCTACAAGTCGGGCCACTCCCGCAGCTCCCGCAAGCACA
  GGGACCGCCGGGACCGACACCGCTCTAAGAGTCGAGATGGGGGCCGAGGGGACAAGTCGGTGACAATCCA
  GGCTCCCGGGGAGCCCCTGCTGGACAATGAGTCCACACGAGGGGATGAGCGGGATGACAACTGGGGGGAA
  ACGACGACAGTAGTAACGGGCACCTCAGAGCACAGCATCTCCCATGATGACCTCACACGCATCGCCAAGG
  ACATGGAGGACAGTGTCCCTCTGGACTGCTCCCGTCACCTGGGTGTGGCAGCGGGGGCCACCCTGGCACT
  GCTGTCTTTCCTCACGCCTCTGGCCTTCCTGCTGCTGCCCCCACTGCTGTGGCGGGAGGAGCTGGAGCCT
  TGCGGGACGGCCTGCGAGGGCCTCTTCATCTCTGTCGCCTTCAAGCTGCTCATCCTGCTACTGGGCAGCT
  GGGCTCTGTTCTTCCGCCGGCCCAAGGCCTCGCTGCCCCGCGTCTTTGTGCTGCGTGCCCTGCTTATGGT
  GCTGGTTTTCCTGCTCGTGGTCTCCTACTGGCTCTTCTATGGTGTGCGCATCCTGGATGCTCGGGAGCGC
  AGCTACCAGGGCGTGGTGCAGTTCGCCGTGTCGCTGGTGGACGCCCTTCTTTTCGTGCACTACCTGGCCG
  TGGTCCTGCTGGAGCTGCGCCAGCTCCAGCCTCAGTTCACGCTCAAGGTCGTGCGCTCCACCGACGGCGC
  CAGCCGCTTCTACAACGTTGGCCATCTCAGCATCCAGCGCGTGGCAGTGTGGATCCTGGAGAAGTATTAC
  CATGACTTCCCTGTCTACAACCCTGCCCTCCTCAACCTGCCCAAGTCCGTCCTGGCCAAGAAAGTGTCTG
  GCTTCAAGGTGTATTCCCTCGGAGAGGAAAACAGCACCAACAACTCCACTGGCCAGTCTCGGGCTGTGAT
  TGCAGCGGCAGCTCGGAGGCGGGACAACAGTCACAATGAGTACTACTATGAGGAGGCTGAGCATGAGCGA
  AGGGTGCGCAAGAGGAGGGCCAGGCTTGTAGTGGCGGTGGAGGAGGCCTTCACTCACATTAAGCGGCTGC
  AGGAAGAGGAGCAGAAAAACCCCAGGGAGGTGATGGACCCCCGGGAGGCAGCCCAAGCCATCTTTGCATC
  CATGGCCCGTGCCATGCAGAAGTACCTTCGGACCACCAAGCAGCAGCCCTACCACACCATGGAGAGCATC
  CTGCAGCACCTTGAATTCTGCATCACGCATGACATGACGCCCAAGGCCTTCTTGGAGCGATACTTGGCGG
  CTGGACCTACCATCCAGTACCACAAGGAACGCTGGCTGGCCAAACAGTGGACATTGGTGAGCGAGGAGCC
  GGTGACCAACGGCCTCAAGGATGGCATCGTTTTCCTCTTAAAACGCCAGGACTTCAGCCTGGTGGTCAGC
  ACCAAGAAGGTCCCATTCTTCAAACTCTCCGAGGAATTTGTGGATCCCAAGTCACACAAGTTTGTCATGA
  GGCTGCAGTCTGAGACCTCAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212173 representing NM_020335
  Red=Cloning site Green=Tags(s)
  
  MDTESQYSGYSYKSGHSRSSRKHRDRRDRHRSKSRDGGRGDKSVTIQAPGEPLLDNESTRGDERDDNWGE
  TTTVVTGTSEHSISHDDLTRIAKDMEDSVPLDCSRHLGVAAGATLALLSFLTPLAFLLLPPLLWREELEP
  CGTACEGLFISVAFKLLILLLGSWALFFRRPKASLPRVFVLRALLMVLVFLLVVSYWLFYGVRILDARER
  SYQGVVQFAVSLVDALLFVHYLAVVLLELRQLQPQFTLKVVRSTDGASRFYNVGHLSIQRVAVWILEKYY
  HDFPVYNPALLNLPKSVLAKKVSGFKVYSLGEENSTNNSTGQSRAVIAAAARRRDNSHNEYYYEEAEHER
  RVRKRRARLVVAVEEAFTHIKRLQEEEQKNPREVMDPREAAQAIFASMARAMQKYLRTTKQQPYHTMESI
  LQHLEFCITHDMTPKAFLERYLAAGPTIQYHKERWLAKQWTLVSEEPVTNGLKDGIVFLLKRQDFSLVVS
  TKKVPFFKLSEEFVDPKSHKFVMRLQSETSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020335
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020335.1, NM_020335.2, NP_065068.1
• RefSeq Size: 5415 bp
• RefSeq ORF: 1566 bp
• Locus ID: 57216
• Protein Families: Transmembrane
• Protein Pathways: Wnt signaling pathway
• MW: 59.5 kDa
• Gene Summary: The protein encoded by this gene is a membrane protein involved in the regulation of planar cell polarity, especially in the stereociliary bundles of the cochlea. The encoded protein transmits directional signals to individual cells or groups of cells in epithelial sheets. This protein is also involved in the development of the neural plate. [provided by RefSeq, Sep 2011]