ATP6V0A4 (NM_020632) Human Tagged ORF Clone

CAT#: RC212179

ATP6V0A4 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a4 (ATP6V0A4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412426) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212179 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ATP6V0A4 protein (Cat# TP312179). The protein was produced from HEK293T cells transfected with ATP6V0A4 cDNA clone (Cat# RC212179) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATP6V0A4
• Synonyms: A4; ATP6N1B; ATP6N2; RDRTA2; RTA1C; RTADR; STV1; VPH1; VPP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212179 representing NM_020632
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTCTGTGTTTCGAAGCGAGGAGATGTGTTTGTCACAACTGTTTCTCCAGGTGGAAGCTGCATATT
  GCTGTGTGGCTGAGCTCGGAGAGCTCGGATTGGTTCAGTTCAAAGATTTAAATATGAATGTGAACAGCTT
  TCAAAGGAAATTTGTGAATGAAGTCAGAAGGTGTGAATCACTGGAGAGAATCCTCCGTTTTCTGGAAGAC
  GAGATGCAAAATGAGATTGTAGTTCAGTTGCTCGAGAAAAGCCCACTGACCCCGCTCCCACGGGAAATGA
  TTACCCTGGAGACTGTTCTAGAAAAACTGGAAGGAGAGTTACAGGAAGCCAACCAGAACCAGCAGGCCTT
  GAAACAAAGCTTCCTAGAACTGACAGAACTGAAATACCTCCTGAAGAAAACCCAAGACTTCTTTGAGACG
  GAAACCAATTTAGCTGATGATTTCTTTACTGAGGACACTTCTGGCCTCCTGGAGTTGAAAGCAGTGCCTG
  CATATATGACCGGAAAGTTGGGGTTCATAGCCGGTGTGATCAACAGGGAGAGGATGGCTTCCTTTGAGCG
  GTTACTGTGGCGAATCTGCCGAGGAAACGTGTACTTGAAGTTCAGTGAGATGGACGCCCCTCTGGAGGAT
  CCTGTGACGAAAGAAGAAATTCAGAAGAACATATTCATCATATTTTACCAAGGAGAGCAGCTCAGGCAGA
  AAATCAAGAAGATCTGTGATGGGTTTCGAGCCACTGTCTACCCTTGCCCAGAGCCTGCGGTGGAGCGCAG
  AGAGATGTTGGAGAGCGTCAATGTGAGGCTGGAAGATTTAATCACCGTCATAACACAAACAGAGTCTCAC
  CGCCAGCGCCTGCTGCAGGAAGCCGCTGCCAACTGGCACTCCTGGCTCATCAAGGTGCAGAAGATGAAAG
  CTGTCTACCACATCCTGAACATGTGCAACATCGACGTCACCCAGCAGTGTGTCATCGCCGAGATCTGGTT
  CCCGGTGGCAGATGCCACACGTATCAAGAGGGCACTGGAGCAAGGCATGGAACTAAGTGGCTCCTCCATG
  GCCCCCATCATGACCACAGTGCAATCTAAAACAGCCCCTCCCACATTTAACAGGACCAATAAATTCACAG
  CTGGCTTCCAGAATATTGTTGATGCCTATGGTGTCGGCAGCTACCGGGAGATAAACCCAGCCCCCTACAC
  CATCATCACTTTCCCCTTCCTGTTCGCTGTGATGTTTGGAGACTGTGGTCATGGAACCGTGATGCTCCTG
  GCTGCACTTTGGATGATTCTGAATGAGAGACGCTTGCTCTCCCAGAAGACAGACAATGAGATTTGGAACA
  CCTTCTTCCACGGGCGCTATCTGATCCTACTTATGGGCATCTTCTCCATCTACACGGGTTTGATCTACAA
  TGACTGCTTCTCCAAGTCCTTGAACATCTTTGGCTCTTCTTGGAGTGTCCAACCCATGTTCAGAAACGGC
  ACATGGAATACTCATGTAATGGAGGAAAGTCTATATCTGCAGCTGGACCCAGCCATACCAGGAGTGTATT
  TTGGAAATCCATACCCGTTTGGGATTGATCCGATTTGGAACTTGGCTTCAAACAAACTCACATTTCTGAA
  CTCGTATAAAATGAAGATGTCGGTGATCCTGGGAATTGTCCAGATGGTTTTCGGTGTCATCCTCAGCCTT
  TTCAATCACATATACTTCAGAAGAACTCTCAACATCATTCTGCAATTTATCCCTGAGATGATTTTTATCC
  TGTGTCTGTTTGGATACCTGGTTTTCATGATCATTTTCAAATGGTGCTGCTTTGACGTCCATGTATCTCA
  GCACGCCCCCAGCATCCTCATCCACTTCATCAACATGTTTCTGTTTAACTACAGTGACTCTTCCAACGCA
  CCCCTCTACAAACATCAGCAAGAAGTCCAAAGTTTCTTTGTGGTTATGGCTTTGATTTCTGTGCCGTGGA
  TGCTTCTGATTAAGCCGTTTATTCTTAGAGCCAGTCATCGGAAATCCCAGCTGCAGGCATCCAGGATCCA
  AGAAGATGCCACTGAGAACATTGAAGGTGATAGCTCCAGCCCTTCTAGCCGTTCTGGCCAGAGGACTTCT
  GCAGATACCCACGGGGCTCTGGACGACCATGGAGAAGAGTTCAACTTTGGAGACGTCTTTGTCCACCAAG
  CCATCCACACCATCGAGTACTGCCTGGGCTGCATTTCAAACACAGCCTCCTACCTGCGGCTCTGGGCCCT
  CAGCCTGGCTCATGCACAACTGTCTGAAGTGCTCTGGACTATGGTGATGAACAGCGGCCTTCAGACGCGA
  GGCTGGGGAGGAATCGTCGGGGTTTTTATTATTTTTGCCGTATTTGCTGTCCTGACAGTAGCCATCCTTC
  TGATCATGGAGGGCCTCTCTGCTTTCCTGCACGCCCTGCGACTGCACTGGGTTGAGTTCCAGAACAAGTT
  CTATGTCGGGGATGGTTACAAGTTTTCTCCATTCTCCTTTAAACACATCCTGGATGGCACAGCCGAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212179 representing NM_020632
  Red=Cloning site Green=Tags(s)
  
  MVSVFRSEEMCLSQLFLQVEAAYCCVAELGELGLVQFKDLNMNVNSFQRKFVNEVRRCESLERILRFLED
  EMQNEIVVQLLEKSPLTPLPREMITLETVLEKLEGELQEANQNQQALKQSFLELTELKYLLKKTQDFFET
  ETNLADDFFTEDTSGLLELKAVPAYMTGKLGFIAGVINRERMASFERLLWRICRGNVYLKFSEMDAPLED
  PVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPEPAVERREMLESVNVRLEDLITVITQTESH
  RQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAEIWFPVADATRIKRALEQGMELSGSSM
  APIMTTVQSKTAPPTFNRTNKFTAGFQNIVDAYGVGSYREINPAPYTIITFPFLFAVMFGDCGHGTVMLL
  AALWMILNERRLLSQKTDNEIWNTFFHGRYLILLMGIFSIYTGLIYNDCFSKSLNIFGSSWSVQPMFRNG
  TWNTHVMEESLYLQLDPAIPGVYFGNPYPFGIDPIWNLASNKLTFLNSYKMKMSVILGIVQMVFGVILSL
  FNHIYFRRTLNIILQFIPEMIFILCLFGYLVFMIIFKWCCFDVHVSQHAPSILIHFINMFLFNYSDSSNA
  PLYKHQQEVQSFFVVMALISVPWMLLIKPFILRASHRKSQLQASRIQEDATENIEGDSSSPSSRSGQRTS
  ADTHGALDDHGEEFNFGDVFVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVMNSGLQTR
  GWGGIVGVFIIFAVFAVLTVAILLIMEGLSAFLHALRLHWVEFQNKFYVGDGYKFSPFSFKHILDGTAEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020632
• ORF Size: 2520 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020632.1, NM_020632.2, NP_065683.1, NP_065683.2
• RefSeq Size: 3137
• RefSeq ORF: 2523
• Locus ID: 50617
• Protein Families: Transmembrane
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• MW: 96.2 kDa
• Gene Summary: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq, Jul 2008]