SIRT4 (NM_012240) Human Tagged ORF Clone

CAT#: RC212226

SIRT4 (Myc-DDK-tagged)-Human sirtuin 4 (SIRT4)

Product Images

Western blot validation of overexpression lysate (Cat# LY402175) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212226 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIRT4
• Synonyms: SIR2L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212226 representing NM_012240
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATGAGCTTTGCGTTGACTTTCAGGTCAGCAAAAGGCCGTTGGATCGCAAACCCCAGCCAGCCGT
  GCTCGAAAGCCTCCATTGGGTTATTTGTGCCAGCAAGTCCTCCTCTGGACCCTGAGAAGGTCAAAGAGTT
  ACAGCGCTTCATCACCCTTTCCAAGAGACTCCTTGTGATGACTGGGGCAGGAATCTCCACCGAATCGGGG
  ATACCAGACTACAGGTCAGAAAAAGTGGGGCTTTATGCCCGCACTGACCGCAGGCCCATCCAGCATGGTG
  ATTTTGTCCGGAGTGCCCCAATCCGCCAGCGGTACTGGGCGAGAAACTTCGTAGGCTGGCCTCAATTCTC
  CTCCCACCAGCCTAACCCTGCACACTGGGCTTTGAGCACCTGGGAGAAACTCGGAAAGCTGTACTGGTTG
  GTGACCCAAAATGTGGATGCTTTGCACACCAAGGCGGGGAGTCGGCGCCTGACAGAGCTCCACGGATGCA
  TGGACAGGGTCCTGTGCTTGGATTGTGGGGAACAGACTCCCCGGGGGGTGCTGCAAGAGCGTTTCCAAGT
  CCTGAACCCCACCTGGAGTGCTGAGGCCCATGGCCTGGCTCCTGATGGTGACGTCTTTCTCTCAGAGGAG
  CAAGTCCGGAGCTTTCAGGTCCCAACCTGCGTTCAATGTGGAGGCCATCTGAAACCAGATGTCGTTTTCT
  TCGGGGACACAGTGAACCCTGACAAGGTTGATTTTGTGCACAAGCGTGTAAAAGAAGCCGACTCCCTCTT
  GGTGGTGGGATCATCCTTGCAGGTATACTCTGGTTACAGGTTTATCCTCACTGCCTGGGAGAAGAAGCTC
  CCGATTGCAATACTGAACATTGGGCCCACACGGTCGGATGACTTGGCGTGTCTGAAACTGAATTCTCGTT
  GTGGAGAGTTGCTGCCTTTGATAGACCCATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212226 representing NM_012240
  Red=Cloning site Green=Tags(s)
  
  MKMSFALTFRSAKGRWIANPSQPCSKASIGLFVPASPPLDPEKVKELQRFITLSKRLLVMTGAGISTESG
  IPDYRSEKVGLYARTDRRPIQHGDFVRSAPIRQRYWARNFVGWPQFSSHQPNPAHWALSTWEKLGKLYWL
  VTQNVDALHTKAGSRRLTELHGCMDRVLCLDCGEQTPRGVLQERFQVLNPTWSAEAHGLAPDGDVFLSEE
  QVRSFQVPTCVQCGGHLKPDVVFFGDTVNPDKVDFVHKRVKEADSLLVVGSSLQVYSGYRFILTAWEKKL
  PIAILNIGPTRSDDLACLKLNSRCGELLPLIDPC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012240
• ORF Size: 942 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012240.1, NM_012240.2, NP_036372.1
• RefSeq Size: 1174 bp
• RefSeq ORF: 945 bp
• Locus ID: 23409
• Domains: SIR2
• Protein Families: Druggable Genome, Transcription Factors
• MW: 35 kDa
• Gene Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]