CYP3A43 (NM_057095) Human Tagged ORF Clone

CAT#: RC212311

CYP3A43 (Myc-DDK-tagged)-Human cytochrome P450, family 3, subfamily A, polypeptide 43 (CYP3A43), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY409258) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212311 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CYP3A43
• Synonyms: MGC119315; MGC119316
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212311 representing NM_057095
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTCATTCCAAACTTTGCCATGGAAACATGGGTTCTTGTGGCTACCAGCCTGGTACTCCTCTATA
  TTTATGGGACCCATTCACATAAACTTTTTAAGAAGCTGGGAATTCCTGGGCCAACCCCTCTGCCTTTTCT
  GGGAACTATTTTGTTCTACCTTAGGGGTCTTTGGAATTTTGACAGAGAATGTAATGAAAAATACGGAGAA
  ATGTGGGGGCTGTATGAGGGGCAACAGCCCATGCTGGTCATCATGGATCCCGACATGATCAAAACAGTGT
  TAGTGAAAGAATGTTACTCTGTCTTCACAAACCAGATGCCTTTAGGTCCAATGGGATTTCTGAAAAGTGC
  CTTAAGTTTTGCTGAAGATGAAGAATGGAAGAGAATACGAACATTGCTATCTCCAGCTTTCACCAGTGTA
  AAATTCAAGGAAATGGTCCCCATCATTTCCCAATGTGGAGATATGTTGGTGAGAAGCCTGAGGCAGGAAG
  CAGAGAACAGCAAGTCCATCAACTTGAAAGATTTCTTTGGGGCCTACACCATGGATGTAATCACTGGCAC
  ATTATTTGGAGTGAACTTGGATTCTCTCAACAATCCACAAGATCCCTTTCTGAAAAATATGAAGAAGCTT
  TTAAAATTGGATTTTTTGGATCCCTTTTTACTCTTAATATCACTCTTTCCATTTCTTACCCCAGTTTTTG
  AAGCCCTAAATATCGGTTTGTTTCCAAAAGATGTTACCCATTTTTTAAAAAATTCCATTGAAAGGATGAA
  AGAAAGTCGCCTCAAAGATAAACAAAAGCATCGAGTAGATTTCTTTCAACAGATGATCGACTCCCAGAAT
  TCCAAAGAAACAAAGTCCCATAAAGCTCTGTCTGATCTGGAGCTTGTGGCCCAGTCAATTATCATCATTT
  TTGCTGCCTATGACACAACTAGCACCACTCTCCCCTTCATTATGTATGAACTGGCCACTCACCCTGATGT
  CCAGCAGAAACTGCAGGAGGAGATTGACGCAGTTTTACCCAATAAGGCACCTGTCACCTACGATGCCCTG
  GTACAGATGGAGTACCTTGACATGGTGGTGAATGAAACGCTCAGATTATTCCCAGTTGTTAGTAGAGTTA
  CGAGAGTCTGCAAGAAAGATATTGAAATCAATGGAGTGTTCATTCCCAAAGGGTTAGCAGTGATGGTTCC
  AATCTATGCTCTTCACCATGACCCAAAGTACTGGACAGAGCCTGAGAAGTTCTGCCCTGAAAGGTTCAGT
  AAGAAGAACAAGGACAGCATAGATCTTTACAGATACATACCTTTTGGAGCTGGACCCCGAAACTGCATTG
  GCATGAGGTTTGCTCTCACAAACATAAAACTTGCTGTCATTAGAGCACTGCAGAACTTCTCCTTCAAACC
  TTGTAAAGAGACTCAGATCCCACTGAAATTAGACAATCTACCAATTCTTCAACCAGAAAAACCTATTGTT
  CTAAAAGTGCACTTAAGAGATGGGATTACAAGTGGACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212311 representing NM_057095
  Red=Cloning site Green=Tags(s)
  
  MDLIPNFAMETWVLVATSLVLLYIYGTHSHKLFKKLGIPGPTPLPFLGTILFYLRGLWNFDRECNEKYGE
  MWGLYEGQQPMLVIMDPDMIKTVLVKECYSVFTNQMPLGPMGFLKSALSFAEDEEWKRIRTLLSPAFTSV
  KFKEMVPIISQCGDMLVRSLRQEAENSKSINLKDFFGAYTMDVITGTLFGVNLDSLNNPQDPFLKNMKKL
  LKLDFLDPFLLLISLFPFLTPVFEALNIGLFPKDVTHFLKNSIERMKESRLKDKQKHRVDFFQQMIDSQN
  SKETKSHKALSDLELVAQSIIIIFAAYDTTSTTLPFIMYELATHPDVQQKLQEEIDAVLPNKAPVTYDAL
  VQMEYLDMVVNETLRLFPVVSRVTRVCKKDIEINGVFIPKGLAVMVPIYALHHDPKYWTEPEKFCPERFS
  KKNKDSIDLYRYIPFGAGPRNCIGMRFALTNIKLAVIRALQNFSFKPCKETQIPLKLDNLPILQPEKPIV
  LKVHLRDGITSGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_057095
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_057095.1, NM_057095.2, NP_476436.1
• RefSeq Size: 2164
• RefSeq ORF: 1512
• Locus ID: 64816
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism
• MW: 57.5 kDa
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein has a low level of testosterone hydroxylase activity, and may play a role in aging mechanisms and cancer progression. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]