PSMB11 (NM_001099780) Human Tagged ORF Clone

CAT#: RC212390

PSMB11 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) subunit, beta type, 11 (PSMB11)

Product Images

Western blot validation of overexpression lysate (Cat# LY420530) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212390 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PSMB11
• Synonyms: BETA5T
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212390 representing NM_001099780
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGCAGGATGTGTGCAAGTGGCAGTCCCCTGACACCCAGGGACCATCACCTCACCTGCCTCGGG
  CTGGCGGCTGGGCTGTGCCCCGGGGTTGTGACCCTCAAACCTTCCTGCAGATCCATGGCCCCAGACTGGC
  CCACGGCACCACCACTCTGGCCTTCCGCTTCCGTCATGGAGTCATTGCTGCAGCTGACACGCGTTCCTCC
  TGTGGCAGCTATGTGGCGTGTCCAGCCTCATGCAAGGTCATCCCTGTGCACCAGCACCTCCTGGGTACCA
  CCTCTGGCACCTCTGCCGACTGTGCTACCTGGTATCGGGTATTACAGCGGGAGCTGCGGCTTCGGGAACT
  GAGGGAGGGTCAGCTGCCCAGTGTGGCCAGTGCTGCCAAGCTCTTGTCAGCCATGATGTCTCAATACCGG
  GGACTGGATCTCTGTGTGGCCACTGCCCTCTGCGGCTGGGACCGCTCTGGCCCTGAGCTCTTCTACGTCT
  ATAGCGACGGCACCCGCCTGCAGGGGGACATCTTCTCTGTGGGCTCTGGATCTCCCTATGCCTACGGCGT
  GCTAGACCGTGGCTATCGCTACGACATGAGCACCCAGGAAGCCTACGCCCTGGCTCGCTGCGCCGTGGCC
  CACGCCACCCACCGTGATGCCTATTCAGGGGGCTCTGTAGACCTTTTCCACGTGCGGGAGAGTGGATGGG
  AGCATGTGTCACGCAGTGATGCCTGTGTGCTGTACGTGGAGTTACAGAAGCTCCTGGAGCCGGAGCCAGA
  GGAGGATGCCAGCCATGCCCATCCTGAGCCTGCCACTGCCCACAGAGCTGCAGAAGATAGAGAGCTCTCT
  GTGGGGCCAGGGGAGGTGACACCAGGAGACTCCAGGATGCCAGCAGGGACTGAGACGGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212390 representing NM_001099780
  Red=Cloning site Green=Tags(s)
  
  MALQDVCKWQSPDTQGPSPHLPRAGGWAVPRGCDPQTFLQIHGPRLAHGTTTLAFRFRHGVIAAADTRSS
  CGSYVACPASCKVIPVHQHLLGTTSGTSADCATWYRVLQRELRLRELREGQLPSVASAAKLLSAMMSQYR
  GLDLCVATALCGWDRSGPELFYVYSDGTRLQGDIFSVGSGSPYAYGVLDRGYRYDMSTQEAYALARCAVA
  HATHRDAYSGGSVDLFHVRESGWEHVSRSDACVLYVELQKLLEPEPEEDASHAHPEPATAHRAAEDRELS
  VGPGEVTPGDSRMPAGTETV
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001099780
• ORF Size: 900 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001099780.1, NP_001093250.1
• RefSeq Size: 1894
• RefSeq ORF: 903
• Locus ID: 122706
• Protein Pathways: Proteasome
• MW: 32.3 kDa
• Gene Summary: Proteasomes generate peptides that are presented by major histocompatibility complex (MHC) I molecules to other cells of the immune system. Proteolysis is conducted by 20S proteasomes, complexes of 28 subunits arranged as a cylinder in 4 heteroheptameric rings: alpha-1 to -7, beta-1 to -7, beta-1 to -7, and alpha-1 to -7. The catalytic subunits are beta-1 (PSMB6; MIM 600307), beta-2 (PSMB7; MIM 604030), and beta-5 (PSMB5; MIM 600306). Three additional subunits, beta-1i (PSMB9; MIM 177045), beta-2i (PSMB10; MIM 176847), and beta-5i (PSMB8; MIM 177046), are induced by gamma-interferon (IFNG; MIM 147570) and are preferentially incorporated into proteasomes to make immunoproteasomes. PSMB11, or beta-5t, is a catalytic subunit expressed exclusively in cortical thymic epithelial cells (Murata et al., 2007 [PubMed 17540904]). [supplied by OMIM, Mar 2008]