MYH (MUTYH) (NM_001048171) Human Tagged ORF Clone

CAT#: RC212561

MUTYH (Myc-DDK-tagged)-Human mutY homolog (E. coli) (MUTYH), nuclear gene encoding mitochondrial protein, transcript variant alpha3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MUTYH
• Synonyms: MYH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212561 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACACCGCTCGTCTCCCGCCTGAGTCGTCTGTGGGCCATCATGAGGAAGCCACGAGCAGCCGTGGGAA
  GTGGTCACAGGAAGCAGGCAGCCAGCCAGGAAGGGAGGCAGAAGCATGCTAAGAACAACAGTCAGGCCAA
  GCCTTCTGCCTGTGATGGCCTGGCCAGGCAGCCGGAAGAGGTGGTATTGCAGGCCTCTGTCTCCTCATAC
  CATCTATTCAGAGACGTAGCTGAAGTCACAGCCTTCCGAGGGAGCCTGCTAAGCTGGTACGACCAAGAGA
  AACGGGACCTACCATGGAGAAGACGGGCAGAAGATGAGATGGACCTGGACAGGCGGGCATATGCTGTGTG
  GGTCTCAGAGGTCATGCTGCAGCAGACCCAGGTTGCCACTGTGATCAACTACTATACCGGATGGATGCAG
  AAGTGGCCTACACTGCAGGACCTGGCCAGTGCTTCCCTGGAGGAGGTGAATCAACTCTGGGCTGGCCTGG
  GCTACTATTCTCGTGGCCGGCGGCTGCAGGAGGGAGCTCGGAAGGTGGTAGAGGAGCTAGGGGGCCACAT
  GCCACGTACAGCAGAGACCCTGCAGCAGCTCCTGCCTGGCGTGGGGCGCTACACAGCTGGGGCCATTGCC
  TCTATCGCCTTTGGCCAGGCAACCGGTGTGGTGGATGGCAACGTAGCACGGGTGCTGTGCCGTGTCCGAG
  CCATTGGTGCTGATCCCAGCAGCACCCTTGTTTCCCAGCAGCTCTGGGGTCTAGCCCAGCAGCTGGTGGA
  CCCAGCCCGGCCAGGAGATTTCAACCAAGCAGCCATGGAGCTAGGGGCCACAGTGTGTACCCCACAGCGC
  CCACTGTGCAGCCAGTGCCCTGTGGAGAGCCTGTGCCGGGCACGCCAGAGAGTGGAGCAGGAACAGCTCT
  TAGCCTCAGGGAGCCTGTCGGGCAGTCCTGACGTGGAGGAGTGTGCTCCCAACACTGGACAGTGCCACCT
  GTGCCTGCCTCCCTCGGAGCCCTGGGACCAGACCCTGGGAGTGGTCAACTTCCCCAGAAAGGCCAGCCGC
  AAGCCCCCCAGGGAGGAGAGCTCTGCCACCTGTGTTCTGGAACAGCCTGGGGCCCTTGGGGCCCAAATTC
  TGCTGGTGCAGAGGCCCAACTCAGGTCTGCTGGCAGGACTGTGGGAGTTCCCGTCCGTGACCTGGGAGCC
  CTCAGAGCAGCTTCAGCGCAAGGCCCTGCTGCAGGAACTACAGCGTTGGGCTGGGCCCCTCCCAGCCACG
  CACCTCCGGCACCTTGGGGAGGTTGTCCACACCTTCTCTCACATCAAGCTGACATATCAAGTATATGGGC
  TGGCCTTGGAAGGGCAGACCCCAGTGACCACCGTACCACCAGGTGCTCGCTGGCTGACGCAGGAGGAATT
  TCACACCGCAGCTGTTTCCACCGCCATGAAAAAGGTTTTCCGTGTGTATCAGGGCCAACAGCCAGGGACC
  TGTATGGGTTCCAAAAGGTCCCAGGTGTCCTCTCCGTGCAGTCGGAAAAAGCCCCGCATGGGCCAGCAAG
  TCCTGGATAATTTCTTTCGGTCTCACATCTCCACTGATGCACACAGCCTCAACAGTGCAGCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212561 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTPLVSRLSRLWAIMRKPRAAVGSGHRKQAASQEGRQKHAKNNSQAKPSACDGLARQPEEVVLQASVSSY
  HLFRDVAEVTAFRGSLLSWYDQEKRDLPWRRRAEDEMDLDRRAYAVWVSEVMLQQTQVATVINYYTGWMQ
  KWPTLQDLASASLEEVNQLWAGLGYYSRGRRLQEGARKVVEELGGHMPRTAETLQQLLPGVGRYTAGAIA
  SIAFGQATGVVDGNVARVLCRVRAIGADPSSTLVSQQLWGLAQQLVDPARPGDFNQAAMELGATVCTPQR
  PLCSQCPVESLCRARQRVEQEQLLASGSLSGSPDVEECAPNTGQCHLCLPPSEPWDQTLGVVNFPRKASR
  KPPREESSATCVLEQPGALGAQILLVQRPNSGLLAGLWEFPSVTWEPSEQLQRKALLQELQRWAGPLPAT
  HLRHLGEVVHTFSHIKLTYQVYGLALEGQTPVTTVPPGARWLTQEEFHTAAVSTAMKKVFRVYQGQQPGT
  CMGSKRSQVSSPCSRKKPRMGQQVLDNFFRSHISTDAHSLNSAAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001048171
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001048171.1, NP_001041636.1
• RefSeq Size: 1905 bp
• RefSeq ORF: 1608 bp
• Locus ID: 4595
• Cytogenetics: 1p34.1
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair
• MW: 59.1 kDa
• Gene Summary: 'This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. This gene product is thought to play a role in signaling apoptosis by the introduction of single-strand breaks following oxidative damage. Mutations in this gene result in heritable predisposition to colorectal cancer, termed MUTYH-associated polyposis (MAP). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017]'