MST4 (STK26) (NM_016542) Human Tagged ORF Clone

CAT#: RC212607

MST4 (Myc-DDK-tagged)-Human serine/threonine protein kinase MST4 (MST4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY413915) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212607 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified STK26 protein (Cat# TP312607). The protein was produced from HEK293T cells transfected with STK26 cDNA clone (Cat# RC212607) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STK26
• Synonyms: MASK; MST4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212607 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCACTCGCCGGTGGCTGTCCAAGTGCCTGGGATGCAGAATAACATAGCTGATCCAGAAGAACTGT
  TCACAAAATTAGAGCGCATTGGGAAAGGCTCATTTGGGGAAGTTTTCAAAGGAATTGATAACCGTACCCA
  GCAAGTCGTTGCTATTAAAATCATAGACCTTGAGGAAGCCGAAGATGAAATAGAAGACATTCAGCAAGAA
  ATAACTGTCTTGAGTCAATGTGACAGCTCATATGTAACAAAATACTATGGGTCATATTTAAAGGGGTCTA
  AATTATGGATAATAATGGAATACCTGGGCGGTGGTTCAGCACTGGATCTTCTTCGAGCTGGTCCATTTGA
  TGAGTTCCAGATTGCTACCATGCTAAAGGAAATTTTAAAAGGTCTGGACTATCTGCATTCAGAAAAGAAA
  ATTCACCGAGACATAAAAGCTGCCAATGTCTTGCTCTCAGAACAAGGAGATGTTAAACTTGCTGATTTTG
  GAGTTGCTGGTCAGCTGACAGATACACAGATTAAAAGAAATACCTTTGTGGGAACTCCATTTTGGATGGC
  TCCTGAAGTTATTCAACAGTCAGCTTATGACTCAAAAGCTGACATTTGGTCATTGGGAATTACTGCTATT
  GAACTAGCCAAGGGAGAGCCACCTAACTCCGATATGCATCCAATGAGAGTTCTGTTTCTTATTCCCAAAA
  ACAATCCTCCAACTCTTGTTGGAGACTTTACTGAGTCTTTTAAGGAGTTTATTGATGCTTGCCTGAACAA
  AGATCCATCATTTCGTCCTACAGCAAAAGAACTTCTGAAACACAAATTCATTGTAAAAAATTCAAAGAAG
  ACTTCTTATCTGACTGAACTGATAGATCGTTTTAAGAGATGGAAGGCAGAAGGACACAGTGATGATGAAT
  CTGATTCCGAGGGCTCTGATTCGGAATCTACCAGCAGGGAAAACAATACTCATCCTGAATGGAGCTTTAC
  CACCGTACGAAAGAAGCCTGATCCAAAGAAAGTACAGAATGGGGCAGAGCAAGATCTTGTGCAAACCCTG
  AGTTGTTTGTCTATGATAATCACACCTGCATTTGCTGAACTTAAACAGCAGGACGAGAATAACGCTAGCA
  GGAATCAGGCGATTGAAGAACTCGAGAAAAGTATTGCTGTGGCTGAAGCCGCCTGTCCCGGCATCACAGA
  TAAAATGGTGAAGAAACTAATTGAAAAATTTCAAAAGTGTTCAGCAGACGAATCCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212607 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAHSPVAVQVPGMQNNIADPEELFTKLERIGKGSFGEVFKGIDNRTQQVVAIKIIDLEEAEDEIEDIQQE
  ITVLSQCDSSYVTKYYGSYLKGSKLWIIMEYLGGGSALDLLRAGPFDEFQIATMLKEILKGLDYLHSEKK
  IHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIQQSAYDSKADIWSLGITAI
  ELAKGEPPNSDMHPMRVLFLIPKNNPPTLVGDFTESFKEFIDACLNKDPSFRPTAKELLKHKFIVKNSKK
  TSYLTELIDRFKRWKAEGHSDDESDSEGSDSESTSRENNTHPEWSFTTVRKKPDPKKVQNGAEQDLVQTL
  SCLSMIITPAFAELKQQDENNASRNQAIEELEKSIAVAEAACPGITDKMVKKLIEKFQKCSADESP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016542
• ORF Size: 1248 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016542.1, NM_016542.2, NM_016542.3, NP_057626.2
• RefSeq Size: 3352 bp
• RefSeq ORF: 1251 bp
• Locus ID: 51765
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 46.5 kDa
• Gene Summary: The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]