GRIK1 (NM_175611) Human Tagged ORF Clone

CAT#: RC212614

GRIK1 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRIK1
• Synonyms: EAA3; EEA3; GLR5; GluK1; gluR-5; GLUR5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212614 representing NM_175611
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCACGGCACACTCCTCGCCCAGCCCGGGCTCTGGACCAGGGACACCAGCTGGGCACTCCTCTATT
  TCCTCTGCTATATCCTCCCTCAGACCGCCCCGCAAGTACTCAGGATCGGAGGGATTTTTGAAACAGTGGA
  AAATGAGCCTGTTAATGTTGAAGAATTAGCTTTCAAGTTTGCAGTCACCAGCATTAACAGAAACCGAACC
  CTGATGCCTAACACCACATTAACCTATGACATCCAGAGAATTAACCTTTTTGATAGTTTTGAAGCCTCGC
  GGAGAGCATGTGACCAGCTGGCTCTTGGTGTGGCTGCTCTCTTTGGCCCTTCCCATAGCTCCTCCGTCAG
  TGCTGTGCAGTCTATTTGCAATGCTCTCGAAGTTCCACACATACAGACCCGCTGGAAACACCCCTCGGTG
  GACAACAAAGATTTGTTTTACATCAACCTTTACCCAGATTATGCAGCTATCAGCAGGGCGATCCTGGATC
  TGGTCCTCTATTACAACTGGAAAACAGTGACAGTGGTGTATGAAGACAGCACAGGTCTAATTCGTCTACA
  AGAGCTCATCAAAGCTCCCTCCAGATATAATATTAAAATCAAAATCCGCCAGCTGCCCTCTGGGAATAAA
  GATGCCAAGCCTTTACTCAAGGAGATGAAGAAAGGCAAGGAGTTCTATGTGATATTTGATTGTTCACATG
  AAACAGCCGCTGAAATCCTTAAGCAGATTCTGTTCATGGGCATGATGACCGAGTACTATCACTACTTTTT
  CACAACCCTGGACTTATTTGCTTTGGATCTGGAACTCTATAGGTACAGTGGCGTAAACATGACCGGGTTT
  CGGCTGCTTAACATTGACAACCCTCACGTGTCATCCATCATTGAGAAGTGGTCCATGGAGAGACTGCAGG
  CCCCACCCAGGCCCGAGACTGGCCTTTTGGATGGCATGATGACAACTGAAGCGGCTCTGATGTACGATGC
  TGTGTACATGGTGGCCATTGCCTCGCACCGGGCATCCCAGCTGACCGTCAGCTCCCTGCAGTGCCATAGA
  CATAAGCCATGGCGCCTCGGACCCAGATTTATGAACCTGATCAAAGAGGCCCGGTGGGATGGCTTGACTG
  GGCATATCACCTTTAATAAAACCAATGGCTTGAGGAAGGATTTTGATCTGGACATTATTAGTCTCAAAGA
  GGAAGGAACTGAAAAGATTGGGATTTGGAATTCCAACAGTGGGCTTAACATGACGGACAGCAACAAAGAC
  AAGTCCAGCAATATCACTGATTCATTGGCCAACAGAACACTCATTGTCACCACCATTCTGGAAGAACCCT
  ATGTTATGTACAGGAAATCTGATAAGCCTCTATATGGAAATGACAGATTTGAAGGATATTGCCTAGACCT
  GTTGAAAGAATTGTCAAACATCCTGGGTTTCATTTATGATGTTAAACTAGTTCCCGATGGCAAATATGGG
  GCCCAGAATGACAAAGGGGAGTGGAACGGGATGGTTAAAGAACTCATAGATCACAGGGCTGACCTGGCAG
  TGGCTCCTCTTACCATCACCTACGTGCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCCTAGG
  CATCAGCATTCTCTACCGGAAGCCCAATGGTACCAATCCAGGCGTTTTCTCCTTCCTCAACCCCCTGTCT
  CCAGATATTTGGATGTATGTGCTCTTAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTGATTGCAAGGT
  TTACACCCTACGAGTGGTATAACCCCCACCCATGCAACCCTGACTCAGACGTGGTGGAAAACAATTTTAC
  TTTACTAAATAGTTTCTGGTTTGGAGTTGGAGCTCTCATGCAGCAAGGATCAGAGCTGATGCCCAAAGCT
  CTATCGACCAGAATAGTTGGAGGGATATGGTGGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCA
  ATCTGGCTGCCTTCTTGACAGTAGAGAGAATGGAATCCCCCATAGATTCGGCAGATGATCTGGCAAAGCA
  AACCAAGATAGAATATGGGGCGGTTAGAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAAATCTCC
  ACCTATGAGAAGATGTGGGCTTTCATGAGCAGCAGGCAGCAGACCGCCCTGGTAAGAAACAGTGATGAGG
  GGATCCAGAGAGTGCTCACCACAGACTACGCGCTGCTGATGGAGTCCACCAGCATTGAGTATGTGACGCA
  GAGAAACTGCAACCTCACTCAGATCGGGGGCCTCATTGACTCCAAAGGTTACGGAGTGGGAACACCTATT
  GGTTCTCCTTACCGGGATAAAATTACTATTGCTATTCTTCAACTCCAAGAAGAAGGGAAGCTGCATATGA
  TGAAAGAGAAGTGGTGGCGTGGGAATGGCTGCCCCGAGGAAGACAACAAAGAAGCCAGTGCCCTGGGAGT
  GGAAAATATTGGAGGCATCTTCATTGTTCTGGCTGCCGGACTGGTCCTTTCTGTATTTGTAGCTATTGGA
  GAATTCATATACAAATCACGGAAGAATAATGATATTGAACAGTGTCTCTCTTTCAACGCTATCATGGAAG
  AACTGGGAATCTCACTGAAGAATCAGAAAAAAATAAAGAAAAAGTCAAGAACTAAGGGGAAATCTTCCTT
  CACAAGTATCCTTACTTGTCATCAGAGACGAACTCAGAGAAAAGAGACTGTGGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212614 representing NM_175611
  Red=Cloning site Green=Tags(s)
  
  MEHGTLLAQPGLWTRDTSWALLYFLCYILPQTAPQVLRIGGIFETVENEPVNVEELAFKFAVTSINRNRT
  LMPNTTLTYDIQRINLFDSFEASRRACDQLALGVAALFGPSHSSSVSAVQSICNALEVPHIQTRWKHPSV
  DNKDLFYINLYPDYAAISRAILDLVLYYNWKTVTVVYEDSTGLIRLQELIKAPSRYNIKIKIRQLPSGNK
  DAKPLLKEMKKGKEFYVIFDCSHETAAEILKQILFMGMMTEYYHYFFTTLDLFALDLELYRYSGVNMTGF
  RLLNIDNPHVSSIIEKWSMERLQAPPRPETGLLDGMMTTEAALMYDAVYMVAIASHRASQLTVSSLQCHR
  HKPWRLGPRFMNLIKEARWDGLTGHITFNKTNGLRKDFDLDIISLKEEGTEKIGIWNSNSGLNMTDSNKD
  KSSNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNILGFIYDVKLVPDGKYG
  AQNDKGEWNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLS
  PDIWMYVLLACLGVSCVLFVIARFTPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQGSELMPKA
  LSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVRDGSTMTFFKKSKIS
  TYEKMWAFMSSRQQTALVRNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNLTQIGGLIDSKGYGVGTPI
  GSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDNKEASALGVENIGGIFIVLAAGLVLSVFVAIG
  EFIYKSRKNNDIEQCLSFNAIMEELGISLKNQKKIKKKSRTKGKSSFTSILTCHQRRTQRKETVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175611
• ORF Size: 2715 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175611.1, NM_175611.2, NP_783300.1
• RefSeq Size: 3489 bp
• RefSeq ORF: 2718 bp
• Locus ID: 2897
• Cytogenetics: 21q21.3
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 99.1 kDa
• Gene Summary: 'Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008]'