GALNT17 (NM_022479) Human Tagged ORF Clone

CAT#: RC212796

WBSCR17 (Myc-DDK-tagged)-Human Williams-Beuren syndrome chromosome region 17 (WBSCR17)

Product Images

Western blot validation of overexpression lysate (Cat# LY402927) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212796 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GALNT17
• Synonyms: GalNAc-T5L; GalNAc-T17; GalNAc-T19; GALNACT17; GALNT16; GALNT20; GALNTL3; WBSCR17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212796 representing NM_022479
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCACTGAGAAGAGTCAAAGTGCTGTTGGTGTTGAACTTGATCGCGGTAGCCGGCTTCGTGCTCT
  TCCTGGCCAAGTGCCGGCCCATCGCGGTGCGCAGCGGAGACGCCTTCCACGAGATCCGGCCGCGCGCCGA
  GGTGGCCAACCTCAGCGCGCACAGCGCCAGCCCCATCCAGGATGCGGTCCTGAAGCGCCTGTCGCTGCTG
  GAGGACATCGTGTACCGGCAGCTGAATGGCTTATCCAAATCCCTTGGGCTCATTGAAGGTTATGGTGGGC
  GGGGTAAAGGGGGCCTTCCGGCTACTCTTTCCCCGGCTGAAGAAGAAAAGGCTAAGGGACCCCATGAGAA
  GTATGGCTACAATTCATACCTCAGTGAAAAAATTTCACTGGACCGTTCCATTCCGGATTATCGTCCCACC
  AAGTGTAAGGAGCTCAAGTACTCCAAGGACCTGCCCCAGATATCCATCATATTCATCTTCGTGAACGAGG
  CCCTGTCGGTGATCCTGCGGTCCGTGCACAGTGCCGTCAATCACACGCCCACACACCTGCTGAAGGAAAT
  CATTCTGGTGGATGACAACAGCGACGAAGAGGAGCTGAAGGTCCCCCTAGAGGAGTATGTCCACAAACGC
  TACCCCGGGCTGGTGAAGGTGGTAAGAAATCAGAAGAGGGAAGGCCTGATCCGCGCTCGCATTGAGGGCT
  GGAAGGTGGCTACCGGGCAGGTCACTGGCTTCTTTGATGCCCACGTGGAATTCACCGCTGGCTGGGCTGA
  GCCGGTTCTATCCCGCATCCAGGAAAACCGGAAGCGTGTGATCCTCCCCTCCATTGACAACATCAAACAG
  GACAACTTTGAGGTGCAGCGGTACGAGAACTCGGCCCACGGGTACAGCTGGGAGCTGTGGTGCATGTACA
  TCAGCCCCCCAAAAGACTGGTGGGACGCCGGAGACCCTTCTCTCCCCATCAGGACCCCAGCCATGATAGG
  CTGCTCGTTCGTGGTCAACAGGAAGTTCTTCGGTGAAATTGGTCTTCTGGATCCTGGCATGGATGTATAC
  GGAGGAGAAAATATTGAACTGGGAATCAAGGTATGGCTCTGTGGGGGCAGCATGGAGGTCCTTCCTTGCT
  CACGGGTGGCCCACATTGAGCGGAAGAAGAAGCCATATAATAGCAACATTGGCTTCTACACCAAGAGGAA
  TGCTCTTCGCGTTGCTGAGGTCTGGATGGACGATTACAAGTCTCATGTGTACATAGCGTGGAACCTGCCG
  CTGGAGAATCCGGGAATTGACATCGGTGATGTCTCCGAAAGAAGAGCATTAAGGAAAAGTTTAAAGTGTA
  AGAATTTCCAGTGGTACCTGGACCATGTTTACCCAGAAATGAGAAGATACAATAATACCGTTGCTTACGG
  GGAGCTTCGCAACAACAAGGCAAAAGACGTCTGCTTGGACCAGGGGCCGCTGGAGAACCACACAGCAATA
  TTGTATCCGTGCCATGGCTGGGGACCACAGCTTGCCCGCTACACCAAGGAAGGCTTCCTGCACTTGGGTG
  CCCTGGGGACCACCACACTCCTCCCTGACACCCGCTGCCTGGTGGACAACTCCAAGAGTCGGCTGCCCCA
  GCTCCTGGACTGCGACAAGGTCAAGAGCAGCCTGTACAAGCGCTGGAACTTCATCCAGAATGGAGCCATC
  ATGAACAAGGGCACGGGACGCTGCCTGGAGGTGGAGAACCGGGGCCTGGCTGGCATCGACCTCATCCTCC
  GCAGCTGCACAGGTCAGAGGTGGACCATTAAGAACTCCATCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212796 representing NM_022479
  Red=Cloning site Green=Tags(s)
  
  MASLRRVKVLLVLNLIAVAGFVLFLAKCRPIAVRSGDAFHEIRPRAEVANLSAHSASPIQDAVLKRLSLL
  EDIVYRQLNGLSKSLGLIEGYGGRGKGGLPATLSPAEEEKAKGPHEKYGYNSYLSEKISLDRSIPDYRPT
  KCKELKYSKDLPQISIIFIFVNEALSVILRSVHSAVNHTPTHLLKEIILVDDNSDEEELKVPLEEYVHKR
  YPGLVKVVRNQKREGLIRARIEGWKVATGQVTGFFDAHVEFTAGWAEPVLSRIQENRKRVILPSIDNIKQ
  DNFEVQRYENSAHGYSWELWCMYISPPKDWWDAGDPSLPIRTPAMIGCSFVVNRKFFGEIGLLDPGMDVY
  GGENIELGIKVWLCGGSMEVLPCSRVAHIERKKKPYNSNIGFYTKRNALRVAEVWMDDYKSHVYIAWNLP
  LENPGIDIGDVSERRALRKSLKCKNFQWYLDHVYPEMRRYNNTVAYGELRNNKAKDVCLDQGPLENHTAI
  LYPCHGWGPQLARYTKEGFLHLGALGTTTLLPDTRCLVDNSKSRLPQLLDCDKVKSSLYKRWNFIQNGAI
  MNKGTGRCLEVENRGLAGIDLILRSCTGQRWTIKNSIK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022479
• ORF Size: 1794 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022479.1, NM_022479.2, NP_071924.1
• RefSeq Size: 3298 bp
• RefSeq ORF: 1797 bp
• Locus ID: 64409
• Domains: RICIN, Glycos_transf_2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• MW: 67.6 kDa
• Gene Summary: This gene encodes an N-acetylgalactosaminyltransferase. This gene is located centromeric to the common deleted region in Williams-Beuren syndrome (WBS), a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. This protein may play a role in membrane trafficking. [provided by RefSeq, Jan 2013]