TOGARAM2 (NM_199280) Human Tagged ORF Clone

CAT#: RC212833

FAM179A (Myc-DDK-tagged)-Human family with sequence similarity 179, member A (FAM179A)

Product Images

Western blot validation of overexpression lysate (Cat# LY404603) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212833 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TOGARAM2 protein (Cat# TP312833). The protein was produced from HEK293T cells transfected with TOGARAM2 cDNA clone (Cat# RC212833) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TOGARAM2
• Synonyms: FAM179A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212833 representing NM_199280
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACCCGTGACGATGTCCCCGAAGCCAAGGTCCTGGTCCCCGTGGCCGTGTACTGCGGGAGCATCC
  CTCGGACCAGTGCTGGGCCCCGGGTGCTCCCGCCTGGAAGCATCAACTCCAGTCTGCCTCATGGAGAAGG
  TTCTCTCCAGCCTGAGCCAAGAGCCCTGCTGAACAACGAGGAACCGTCACAGCTCCTGCGTGGACTCGGA
  CAGCTGGGTGGCCTCAAGCTGGACACCCCTTCCAAGGGCTGGCAGGCAAGGAATGGTCACCCCAGGAACC
  TCAGGGCCTTGTCTTTGGGGGACCAGCCCCTGGTGCTCCTCCCTTCTCCGGAGTCAGAGGCCAACAGCGT
  GGCCAGGGACACCATCCAGATTAAGGACAAGCTCAAGAAAAGGAGGCTCTCAGAGGGCTTGGCAGCGTCT
  TCCCGAGCCTCTCTGGATCCAGGGGGAGGCCCCCAAGGAGTTCCCCTGCACAGCACCATCCCCCGAGCCA
  CCTCTCAGAGGCTGCTGAGGGTGCCCAGGCCGATGCCTCTCATCCAGAGCATCCCTACCACCCCTGAGGC
  CAGCGGAGTCAAAGAGAAGGGCCTGGACCTACCGGGGAGCATTCCGGGTCCTCACGAGTTGAGACCCGGT
  GCTCAGGAGGCGCAGATCTCCTGGCAATACCTGCACTGCAATGATGAGAAGATGCAGAAGTCCCTGGGCG
  CCATCGTGATCCCACCCATCCCAAAGGCCAGGACGGTTGCAGCGACCCCCTCCCGTGTGCCTGGCTCCCT
  TCCCAGCCCGTTACCTCCAGGCCAGGGAGTCCTCACAGGCCTGAGGGCCCCACGCACGCGATTGGCTCGG
  GGGAGTGGGCCTCGGGAGAAGACCCCTGCATCTCTGGAGCCAAAACCTTTGGCCTCACCCATCAGAGACA
  GGCCTGCCGCTGCCAAGAAGCCTGCCCTGCCTTTTTCTCAGTCTGCTCCCACGCTGACAGCCTTCTCCTT
  TGACTGTGCCAGAGAAGCCTGCCCTCCGCTGAAAGAAGAGGACCAGAAGGAGATCGGCACCAAGATCCAA
  GTCACCATCTCCAAGTCTGCCCGGGAGAAGATGCAGCTGAAGCAGATGAAGGAGATGGAGCTGCTTCGGA
  GGCTGGAGGAGCCCAGGACAGGGCAGGAGCTCACTTCCCAGTGCCTGGGCTCCCAGAGAGCCTTCATGAA
  GGAAGGCCTCCTTCCCCTCCGGGGCAGCGGGACACTGTCTGTGCCCACTAGGCTGAGCGGCCCATGCAGA
  AACGACGTCAGCATCATCCTGAGGAAGTGGGCCAGCCGGGCCTCCCTGCCCAGCATCCCCATCAGCCGGC
  AGGAGCCCCGCTTTGCCCGCCACGCCTCAGCTAACTCATTACCTGCGGTGCTCACGTTGGGGTCTCCTGA
  ATGGGAAGAAGAGGAGGAGATGGATCTTAGAGCCTGTAAGGAGTTGAGGCCTTTCTCGAACCCGGAGCTG
  GGGCTGAGGGATGCACTCCAGTGCCTCAACAGCAGTGACTGGCAGATGAAGGAGAAGGGTCTGGTGAGCA
  TCCAGCGCTTGGCAGCCTGTCACTCAGAGGTCCTCACCGGGAAGCTGCACGACGTGTGCTTGGTGGTGAC
  TGGGGAGGTCACCAACCTGCGGTCCAAGGTGTCTCACCTGGCCATCAGCACCTTGGGAGACCTCTTCCAG
  GCCTTGAAGAAGAATATGGACCAGGAGGCCGAGGAGATCGCCCGCTGCTTGCTGCAGAAGATGGCGGACA
  CCAACGAGTTCATCCAGAGAGCAGCCGGCCAGTCTCTGAGGGCTATGGTGGAGAATGTGACCCTTGCCCG
  CTCCCTGGTGGTCCTCACCTCGGCGGGTGTCTACCACCGGAACCCCTTGATCCGGAAATACGCGGCTGAG
  CACCTCTCAGCTGTGCTGGAGCAGATCGGCGCTGAGAAGCTTCTCTCGGGCACCAGAGACAGCACAGACA
  TGTTGGTGCACAACCTGGTGAGGCTGGCACAGGACTCCAACCAGGACACCAGATTTTATGGCCGGAAGAT
  GGTGAATATCTTGATGGCGAACACTAAGTTTGATGCATTTCTGAAGCAATCTCTCCCATCTTACGACTTG
  CAGAAGGTCATGGCGGCCATTAAACAGCAGGGAATAGAAGATAATGATGAACTTCCCTCTGCCAAAGGCC
  GCAAGGTGTTGAGGAGTCTGGTGGTGTGTGAGAACGGGCTGCCCATCAAGGAGGGGCTCAGCTGCAATGG
  CCCAAGGCTGGTGGGGCTGCGCTCCACACTGCAGGGCCGCGGGGAGATGGTGGAGCAGCTACGGGAGCTG
  ACACGGCTGCTGGAGGCCAAGGACTTCCGGTCCCGGATGGAAGGCGTGGGGCAGCTCCTGGAGCTCTGCA
  AGGCCAAGACGGAGCTTGTCACTGCCCACCTGGTCCAGGTCTTTGATGCTTTCACCCCAAGGCTTCAGGA
  TTCCAACAAGAAAGTGAACCAGTGGGCGCTGGAGTCCTTCGCCAAGATGATCCCCCTCCTCAGAGAGAGC
  TTACACCCCATGCTGCTCTCCATCATCATCACTGTTGCAGACAACCTCAACTCCAAGAACTCAGGGATTT
  ACGCTGCTGCCGTGGCTGTGCTGGATGCGATGGTTGAGAGCCTGGACAACCTTTGCCTTCTACCAGCGCT
  TGCTGGGCGAGTGCGTTTCCTGAGTGGCCGTGCGGTGCTGGATGTCACAGATCGCCTGGCAGTGCTGGTG
  GCCTCAGTTTACCCCCGGAAGCCTCAAGCTGTAGAGCGGCATGTCCTTCCCATCCTCTGGCACTTCCTGA
  ACACCGCCACCAGGAATGGCACCCTGCCTGGACCCAGCGGGAACATCCGCGGGGTGGTGTGCCGGCTGTC
  CAGGAGCCTCCAGGAGCACATGGGCTCCCGCCTGCTGGACTTTGCCGCCAGCCAGCCAAAGCACGTCCTC
  AAGACGCTCCAGGAACTCTTAGACTCAGAGTCCTTGGGAGGCAGCCGCAAGGCCACTGACAGAGGGGTGG
  CCCCTGACAGCAAGACAACTGGCAGCTCATACCCTTTTCAGCTGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212833 representing NM_199280
  Red=Cloning site Green=Tags(s)
  
  MGTRDDVPEAKVLVPVAVYCGSIPRTSAGPRVLPPGSINSSLPHGEGSLQPEPRALLNNEEPSQLLRGLG
  QLGGLKLDTPSKGWQARNGHPRNLRALSLGDQPLVLLPSPESEANSVARDTIQIKDKLKKRRLSEGLAAS
  SRASLDPGGGPQGVPLHSTIPRATSQRLLRVPRPMPLIQSIPTTPEASGVKEKGLDLPGSIPGPHELRPG
  AQEAQISWQYLHCNDEKMQKSLGAIVIPPIPKARTVAATPSRVPGSLPSPLPPGQGVLTGLRAPRTRLAR
  GSGPREKTPASLEPKPLASPIRDRPAAAKKPALPFSQSAPTLTAFSFDCAREACPPLKEEDQKEIGTKIQ
  VTISKSAREKMQLKQMKEMELLRRLEEPRTGQELTSQCLGSQRAFMKEGLLPLRGSGTLSVPTRLSGPCR
  NDVSIILRKWASRASLPSIPISRQEPRFARHASANSLPAVLTLGSPEWEEEEEMDLRACKELRPFSNPEL
  GLRDALQCLNSSDWQMKEKGLVSIQRLAACHSEVLTGKLHDVCLVVTGEVTNLRSKVSHLAISTLGDLFQ
  ALKKNMDQEAEEIARCLLQKMADTNEFIQRAAGQSLRAMVENVTLARSLVVLTSAGVYHRNPLIRKYAAE
  HLSAVLEQIGAEKLLSGTRDSTDMLVHNLVRLAQDSNQDTRFYGRKMVNILMANTKFDAFLKQSLPSYDL
  QKVMAAIKQQGIEDNDELPSAKGRKVLRSLVVCENGLPIKEGLSCNGPRLVGLRSTLQGRGEMVEQLREL
  TRLLEAKDFRSRMEGVGQLLELCKAKTELVTAHLVQVFDAFTPRLQDSNKKVNQWALESFAKMIPLLRES
  LHPMLLSIIITVADNLNSKNSGIYAAAVAVLDAMVESLDNLCLLPALAGRVRFLSGRAVLDVTDRLAVLV
  ASVYPRKPQAVERHVLPILWHFLNTATRNGTLPGPSGNIRGVVCRLSRSLQEHMGSRLLDFAASQPKHVL
  KTLQELLDSESLGGSRKATDRGVAPDSKTTGSSYPFQLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199280
• ORF Size: 3057 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199280.2, NP_954974.2
• RefSeq Size: 3564
• RefSeq ORF: 3060
• Locus ID: 165186
• MW: 111 kDa