Fibronectin (FN1) (NM_054034) Human Tagged ORF Clone
CAT#: RC212860L2
- LentiORF®
Lenti ORF clone of Human fibronectin 1 (FN1), transcript variant 7, mGFP tagged
"NM_054034" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | FN1 |
Synonyms | CIG; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF; SMDCF |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC212860).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_054034 |
ORF Size | 1971 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_054034.2, NP_473375.2 |
RefSeq Size | 2402 bp |
RefSeq ORF | 1974 bp |
Locus ID | 2335 |
Cytogenetics | 2q35 |
Domains | FN1, FN2 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein |
Protein Pathways | ECM-receptor interaction, Focal adhesion, Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer |
MW | 71.2 kDa |
Gene Summary | 'This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC314343 | FN1 (untagged)-Human fibronectin 1 (FN1), transcript variant 7 |
USD 1,110.00 |
|
RC212860 | FN1 (Myc-DDK-tagged)-Human fibronectin 1 (FN1), transcript variant 7 |
USD 480.00 |
|
RG212860 | FN1 (GFP-tagged) - Human fibronectin 1 (FN1), transcript variant 7 |
USD 530.00 |
|
RC212860L1 | Lenti ORF clone of Human fibronectin 1 (FN1), transcript variant 7, Myc-DDK-tagged |
USD 840.00 |
|
RC212860L3 | Lenti ORF clone of Human fibronectin 1 (FN1), transcript variant 7, Myc-DDK-tagged |
USD 680.00 |
|
RC212860L4 | Lenti ORF clone of Human fibronectin 1 (FN1), transcript variant 7, mGFP tagged |
USD 680.00 |
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