HDAC5 (NM_005474) Human Tagged ORF Clone

CAT#: RC212863

HDAC5 (Myc-DDK-tagged)-Human histone deacetylase 5 (HDAC5), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HDAC5
• Synonyms: HD5; NY-CO-9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212863 representing NM_005474
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCTCCCAACGAGTCGGATGGGATGTCAGGTCGGGAACCATCCTTGGAAATCCTGCCGCGGACTT
  CTCTGCACAGCATCCCTGTGACAGTGGAGGTGAAGCCGGTGCTGCCAAGAGCCATGCCCAGTTCCATGGG
  GGGTGGGGGTGGAGGCAGCCCCAGCCCTGTGGAGCTACGGGGGGCTCTGGTGGGCTCTGTGGACCCCACA
  CTGCGGGAGCAGCAACTGCAGCAGGAGCTCCTGGCGCTCAAGCAGCAGCAGCAGCTGCAGAAGCAGCTCC
  TGTTCGCTGAGTTCCAGAAACAGCATGACCACCTGACAAGGCAGCATGAGGTCCAGCTGCAGAAGCACCT
  CAAGCAGCAGCAGGAGATGCTGGCAGCCAAGCAGCAGCAGGAGATGCTGGCAGCCAAGCGGCAGCAGGAG
  CTGGAGCAGCAGCGGCAGCGGGAGCAGCAGCGGCAGGAAGAGCTGGAGAAGCAGCGGCTGGAGCAGCAGC
  TGCTCATCCTGCGGAACAAGGAGAAGAGCAAAGAGAGTGCCATTGCCAGCACTGAGGTAAAGCTGAGGCT
  CCAGGAATTCCTCTTGTCGAAGTCAAAGGAGCCCACACCAGGCGGCCTCAACCATTCCCTCCCACAGCAC
  CCCAAATGCTGGGGAGCCCACCATGCTTCTTTGGACCAGAGTTCCCCTCCCCAGAGCGGCCCCCCTGGGA
  CGCCTCCCTCCTACAAACTGCCTTTGCCTGGGCCCTACGACAGTCGAGACGACTTCCCCCTCCGCAAAAC
  AGCCTCTGAACCCAACTTGAAAGTGCGTTCAAGGCTAAAACAGAAGGTGGCTGAGCGGAGAAGCAGTCCC
  CTCCTGCGTCGCAAGGATGGGACTGTTATTAGCACCTTTAAGAAGAGAGCTGTTGAGATCACAGGTGCCG
  GGCCTGGGGCGTCGTCCGTGTGTAACAGCGCACCCGGCTCCGGCCCCAGCTCTCCCAACAGCTCCCACAG
  CACCATCGCTGAGAATGGCTTTACTGGCTCAGTCCCCAACATCCCCACTGAGATGCTCCCTCAGCACCGA
  GCCCTCCCTCTGGACAGCTCCCCCAACCAGTTCAGCCTCTACACGTCTCCTTCTCTGCCCAACATCTCCC
  TAGGGCTGCAGGCCACGGTCACTGTCACCAACTCACACCTCACTGCCTCCCCGAAGCTGTCGACACAGCA
  GGAGGCCGAGAGGCAGGCCCTCCAGTCCCTGCGGCAGGGTGGCACGCTGACCGGCAAGTTCATGAGCACA
  TCCTCTATTCCTGGCTGCCTGCTGGGCGTGGCACTGGAGGGCGACGGGAGCCCCCACGGGCATGCCTCCC
  TGCTGCAGCATGTGCTGTTGCTGGAGCAGGCCCGGCAGCAGAGCACCCTCATTGCTGTGCCACTCCACGG
  GCAGTCCCCACTAGTGACGGGTGAACGTGTGGCCACCAGCATGCGGACGGTAGGCAAGCTCCCGCGGCAT
  CGGCCCCTGAGCCGCACTCAGTCCTCACCGCTGCCGCAGAGTCCCCAGGCCCTGCAGCAGCTGGTCATGC
  AACAACAGCACCAGCAGTTCCTGGAGAAGCAGAAGCAGCAGCAGCTACAGCTGGGCAAGATCCTCACCAA
  GACAGGGGAGCTGCCCAGGCAGCCCACCACCCACCCTGAGGAGACAGAGGAGGAGCTGACGGAGCAGCAG
  GAGGTCTTGCTGGGGGAGGGAGCCCTGACCATGCCCCGGGAGGGCTCCACAGAGAGTGAGAGCACACAGG
  AAGACCTGGAGGAGGAGGACGAGGAAGACGATGGGGAGGAGGAGGAGGATTGCATCCAGGTTAAGGACGA
  GGAGGGCGAGAGTGGTGCTGAGGAGGGGCCCGACTTGGAGGAGCCTGGTGCTGGATACAAAAAACTGTTC
  TCAGATGCCCAGCCGCTGCAGCCTTTGCAGGTGTACCAGGCGCCCCTCAGCCTGGCCACTGTGCCCCACC
  AGGCCCTGGGCCGTACCCAGTCCTCCCCTGCTGCCCCTGGGGGCATGAAGAGCCCCCCAGACCAGCCCGT
  CAAGCACCTCTTCACCACAGGTGTGGTCTACGACACGTTCATGCTAAAGCACCAGTGCATGTGCGGGAAC
  ACACACGTGCACCCTGAGCATGCTGGCCGGATCCAGAGCATCTGGTCCCGGCTGCAGGAGACAGGCCTGC
  TTAGCAAGTGCGAGCGGATCCGAGGTCGCAAAGCCACGCTAGATGAGATCCAGACAGTGCACTCTGAATA
  CCACACCCTGCTCTATGGGACCAGTCCCCTCAACCGGCAGAAGCTAGACAGCAAGAAGTTGCTCGGCCCC
  ATCAGCCAGAAGATGTATGCTGTGCTGCCTTGTGGGGGCATCGGGGTGGACAGTGACACCGTGTGGAATG
  AGATGCACTCCTCCAGTGCTGTGCGCATGGCAGTGGGCTGCCTGCTGGAGCTGGCCTTCAAGGTGGCTGC
  AGGAGAGCTCAAGAATGGATTTGCCATCATCCGGCCCCCAGGACACCACGCCGAGGAATCCACAGCCATG
  GGATTCTGCTTCTTCAACTCTGTAGCCATCACCGCAAAACTCCTACAGCAGAAGTTGAACGTGGGCAAGG
  TCCTCATCGTGGACTGGGACATTCACCATGGCAATGGCACCCAGCAGGCGTTCTACAATGACCCCTCTGT
  GCTCTACATCTCTCTGCATCGCTATGACAACGGGAACTTCTTTCCAGGCTCTGGGGCTCCTGAAGAGGTT
  GGTGGAGGACCAGGCGTGGGGTACAATGTGAACGTGGCATGGACAGGAGGTGTGGACCCCCCCATTGGAG
  ACGTGGAGTACCTTACAGCCTTCAGGACAGTGGTGATGCCCATTGCCCACGAGTTCTCACCTGATGTGGT
  CCTAGTCTCCGCCGGGTTTGATGCTGTTGAAGGACATCTGTCTCCTCTGGGTGGCTACTCTGTCACCGCC
  AGATGTTTTGGCCACTTGACCAGGCAGCTGATGACCCTGGCAGGGGGCCGGGTGGTGCTGGCCCTGGAGG
  GAGGCCATGACTTGACCGCCATCTGTGATGCCTCTGAGGCTTGTGTCTCGGCTCTGCTCAGTGTAGAGCT
  GCAGCCCTTGGATGAGGCAGTCTTGCAGCAAAAGCCCAACATCAACGCAGTGGCCACGCTAGAGAAAGTC
  ATCGAGATCCAGAGCAAACACTGGAGCTGTGTGCAGAAGTTCGCCGCTGGTCTGGGCCGGTCCCTGCGAG
  AGGCCCAAGCAGGTGAGACCGAGGAGGCCGAGACTGTGAGCGCCATGGCCTTGCTGTCGGTGGGGGCCGA
  GCAGGCCCAGGCTGCGGCAGCCCGGGAACACAGCCCCAGGCCGGCAGAGGAGCCCATGGAGCAGGAGCCT
  GCCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212863 representing NM_005474
  Red=Cloning site Green=Tags(s)
  
  MNSPNESDGMSGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELRGALVGSVDPT
  LREQQLQQELLALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQEMLAAKQQQEMLAAKRQQE
  LEQQRQREQQRQEELEKQRLEQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQH
  PKCWGAHHASLDQSSPPQSGPPGTPPSYKLPLPGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSP
  LLRRKDGTVISTFKKRAVEITGAGPGASSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMLPQHR
  ALPLDSSPNQFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGGTLTGKFMST
  SSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATSMRTVGKLPRH
  RPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQLQLGKILTKTGELPRQPTTHPEETEEELTEQQ
  EVLLGEGALTMPREGSTESESTQEDLEEEDEEDDGEEEEDCIQVKDEEGESGAEEGPDLEEPGAGYKKLF
  SDAQPLQPLQVYQAPLSLATVPHQALGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGN
  THVHPEHAGRIQSIWSRLQETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGP
  ISQKMYAVLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAM
  GFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEV
  GGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVSAGFDAVEGHLSPLGGYSVTA
  RCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPNINAVATLEKV
  IEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEP
  AL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005474
• ORF Size: 3366 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005474.1, NM_005474.2, NM_005474.3, NM_005474.4, NP_005465.2
• RefSeq Size: 5324
• RefSeq ORF: 3369
• Locus ID: 10014
• Domains: Hist_deacetyl
• Protein Families: Druggable Genome, Transcription Factors
• MW: 121.8 kDa
• Gene Summary: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]