C14orf43 (ELMSAN1) (NM_194278) Human Tagged ORF Clone

CAT#: RC212903

ELMSAN1 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 43 (C14orf43), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY420815) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217065 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ELMSAN1
• Synonyms: C14orf43; C14orf117; c14_5541; LSR68; MIDEAS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212903 representing NM_194278
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTCCAGGCCCAGCCCAAGGCTCAGAACAAGCGGAAGCGTTGCCTCTTCGGGGGCCAGGAACCAG
  CTCCCAAGGAGCAGCCCCCTCCCCTGCAGCCCCCCCAGCAGTCCATCAGAGTGAAGGAGGAGCAGTACCT
  CGGGCACGAGGGTCCAGGAGGGGCAGTCTCCACCTCTCAGCCTGTGGAACTGCCCCCTCCTAGCAGCCTG
  GCCCTGCTGAACTCTGTGGTATATGGGCCTGAGCGGACCTCAGCAGCCATGCTGTCCCAGCAGGTGGCCT
  CAGTAAAGTGGCCCAACTCTGTGATGGCTCCAGGGCGGGGCCCGGAGCGTGGAGGAGGTGGGGGTGTCAG
  TGACAGCAGCTGGCAGCAGCAGCCAGGCCAGCCTCCACCCCATTCAACATGGAACTGCCACAGTCTGTCC
  CTCTACAGTGCAACCAAGGGGAGCCCGCATCCTGGAGTGGGAGTCCCGACTTACTATAACCACCCTGAGG
  CACTGAAGCGGGAGAAAGCGGGGGGCCCACAGCTGGACCGCTATGTGCGACCAATGATGCCACAGAAGGT
  GCAGCTGGAGGTAGGGCGGCCCCAGGCACCCCTGAATTCTTTCCACGCAGCCAAGAAACCCCCAAACCAG
  TCACTGCCCCTGCAACCCTTCCAGCTGGCATTCGGCCACCAGGTGAACCGGCAGGTCTTCCGGCAGGGCC
  CACCGCCCCCAAACCCGGTGGCTGCCTTCCCTCCACAGAAGCAGCAGCAGCAGCAGCAACCACAGCAGCA
  GCAGCAGCAGCAGCAGGCAGCCCTACCCCAGATGCCGCTCTTTGAGAACTTCTATTCCATGCCGCAGCAA
  CCCTCGCAGCAACCCCAGGACTTTGGCCTGCAGCCAGCTGGGCCACTGGGACAGTCCCACCTGGCTCACC
  ACAGCATGGCACCCTACCCCTTCCCCCCCAACCCAGATATGAACCCAGAACTGCGCAAGGCCCTTCTGCA
  GGACTCAGCCCCGCAGCCAGCGCTACCTCAGGTCCAGATCCCCTTCCCCCGCCGCTCCCGCCGCCTCTCT
  AAGGAGGGTATCCTGCCTCCCAGCGCCCTGGATGGGGCTGGCACCCAGCCTGGGCAGGAGGCCACTGGCA
  ACCTGTTCCTACATCACTGGCCCCTGCAGCAGCCGCCACCTGGCTCCCTGGGGCAGCCCCATCCTGAAGC
  TCTGGGATTCCCGCTGGAGCTGAGGGAGTCGCAGCTACTGCCTGATGGGGAGAGACTAGCACCCAATGGC
  CGGGAGCGAGAGGCTCCTGCCATGGGCAGCGAGGAGGGCATGAGGGCAGTGAGCACAGGGGACTGTGGGC
  AGGTGCTACGGGGCGGAGTGATCCAGAGCACGCGACGGAGGCGCCGGGCATCCCAGGAGGCCAATTTGCT
  GACCCTGGCCCAGAAGGCTGTGGAGCTGGCCTCACTGCAGAATGCAAAGGATGGCAGTGGTTCTGAAGAG
  AAGCGGAAAAGTGTATTGGCCTCAACTACCAAGTGTGGGGTGGAGTTTTCTGAGCCTTCCTTAGCCACCA
  AGCGAGCACGAGAAGACAGTGGGATGGTACCCCTCATCATCCCAGTGTCTGTGCCTGTGCGAACTGTGGA
  CCCAACTGAGGCAGCCCAGGCTGGAGGTCTTGATGAGGACGGGAAGGGTCCTGAACAGAACCCTGCTGAG
  CACAAGCCATCAGTCATCGTCACCCGCAGGCGGTCCACCCGAATCCCCGGGACAGATGCTCAAGCTCAGG
  CAGAGGACATGAATGTCAAGTTGGAGGGGGAGCCTTCCGTGCGGAAACCAAAGCAGCGGCCCAGGCCCGA
  GCCCCTCATCATCCCCACCAAGGCGGGCACTTTCATCGCCCCTCCCGTCTACTCCAACATCACCCCATAC
  CAGAGCCACCTGCGCTCTCCCGTGCGCCTAGCTGACCACCCCTCTGAGCGGAGCTTTGAGCTACCTCCCT
  ACACGCCGCCCCCCATCCTCAGCCCTGTGCGGGAAGGCTCTGGCCTCTACTTCAATGCCATCATATCAAC
  CAGCACCATCCCTGCCCCTCCTCCCATCACGCCTAAGAGTGCCCATCGCACGCTGCTCCGGACTAACAGT
  GCTGAAGTAACCCCGCCTGTCCTCTCTGTGATGGGGGAGGCCACCCCAGTGAGCATCGAGCCACGGATCA
  ACGTGGGCTCCCGGTTCCAGGCAGAAATCCCCTTGATGAGGGACCGTGCCCTGGCAGCTGCAGATCCCCA
  CAAGGCTGACTTGGTGTGGCAGCCATGGGAGGACCTAGAGAGCAGCCGGGAGAAGCAGAGGCAAGTGGAA
  GACCTGCTGACAGCCGCCTGCTCCAGCATTTTCCCTGGTGCTGGCACCAACCAGGAGCTGGCCCTGCACT
  GTCTGCACGAATCCAGAGGAGACATCCTGGAAACGCTGAATAAGCTGCTGCTGAAGAAGCCCCTGCGGCC
  CCACAACCATCCGCTGGCAACTTATCACTACACAGGCTCTGACCAGTGGAAGATGGCCGAGAGGAAGCTG
  TTCAACAAAGGCATTGCCATCTACAAGAAGGATTTCTTCCTGGTGCAGAAGCTGATCCAGACCAAGACCG
  TGGCCCAGTGCGTGGAGTTCTACTACACCTACAAGAAGCAGGTGAAAATCGGCCGCAATGGGACTCTAAC
  CTTTGGGGATGTGGATACGAGCGATGAGAAGTCGGCCCAGGAAGAGGTTGAAGTGGATATTAAGACTTCC
  CAAAAGTTCCCAAGGGTGCCTCTTCCCAGAAGAGAGTCCCCAAGTGAAGAGAGGCTGGAGCCCAAGAGGG
  AGGTGAAGGAGCCCAGGAAGGAGGGGGAGGAGGAGGTGCCAGAGATCCAAGAGAAGGAGGAGCAGGAAGA
  GGGGCGAGAGCGCAGCAGGCGGGCAGCGGCAGTCAAAGCCACGCAGACACTACAGGCCAATGAGTCGGCC
  AGTGACATCCTCATCCTCCGGAGCCACGAGTCCAACGCCCCTGGGTCTGCCGGTGGCCAGGCCTCGGAGA
  AGCCAAGGGAAGGGACAGGGAAGTCACGAAGGGCACTACCTTTTTCAGAGAAGAAGAAAAAAACAGAGAC
  ATTCAGTAAGACCCAGAATCAGGAGAACACTTTCCCCTGTAAAAAATGTGGCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212903 representing NM_194278
  Red=Cloning site Green=Tags(s)
  
  MNLQAQPKAQNKRKRCLFGGQEPAPKEQPPPLQPPQQSIRVKEEQYLGHEGPGGAVSTSQPVELPPPSSL
  ALLNSVVYGPERTSAAMLSQQVASVKWPNSVMAPGRGPERGGGGGVSDSSWQQQPGQPPPHSTWNCHSLS
  LYSATKGSPHPGVGVPTYYNHPEALKREKAGGPQLDRYVRPMMPQKVQLEVGRPQAPLNSFHAAKKPPNQ
  SLPLQPFQLAFGHQVNRQVFRQGPPPPNPVAAFPPQKQQQQQQPQQQQQQQQAALPQMPLFENFYSMPQQ
  PSQQPQDFGLQPAGPLGQSHLAHHSMAPYPFPPNPDMNPELRKALLQDSAPQPALPQVQIPFPRRSRRLS
  KEGILPPSALDGAGTQPGQEATGNLFLHHWPLQQPPPGSLGQPHPEALGFPLELRESQLLPDGERLAPNG
  REREAPAMGSEEGMRAVSTGDCGQVLRGGVIQSTRRRRRASQEANLLTLAQKAVELASLQNAKDGSGSEE
  KRKSVLASTTKCGVEFSEPSLATKRAREDSGMVPLIIPVSVPVRTVDPTEAAQAGGLDEDGKGPEQNPAE
  HKPSVIVTRRRSTRIPGTDAQAQAEDMNVKLEGEPSVRKPKQRPRPEPLIIPTKAGTFIAPPVYSNITPY
  QSHLRSPVRLADHPSERSFELPPYTPPPILSPVREGSGLYFNAIISTSTIPAPPPITPKSAHRTLLRTNS
  AEVTPPVLSVMGEATPVSIEPRINVGSRFQAEIPLMRDRALAAADPHKADLVWQPWEDLESSREKQRQVE
  DLLTAACSSIFPGAGTNQELALHCLHESRGDILETLNKLLLKKPLRPHNHPLATYHYTGSDQWKMAERKL
  FNKGIAIYKKDFFLVQKLIQTKTVAQCVEFYYTYKKQVKIGRNGTLTFGDVDTSDEKSAQEEVEVDIKTS
  QKFPRVPLPRRESPSEERLEPKREVKEPRKEGEEEVPEIQEKEEQEEGRERSRRAAAVKATQTLQANESA
  SDILILRSHESNAPGSAGGQASEKPREGTGKSRRALPFSEKKKKTETFSKTQNQENTFPCKKCGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194278
• ORF Size: 3135 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194278.2, NM_194278.3, NP_919254.2
• RefSeq Size: 8105 bp
• RefSeq ORF: 3138 bp
• Locus ID: 91748
• MW: 114.8 kDa