C10orf81 (PLEKHS1) (NM_024889) Human Tagged ORF Clone

CAT#: RC212940

PLEKHS1 (Myc-DDK-tagged)-Human chromosome 10 open reading frame 81 (C10orf81), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY410994) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212940 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLEKHS1
• Synonyms: C10orf81; HEL185
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212940 representing NM_024889
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCCAAACCTCAGAAGAGTCCAGGCAAACAATTTACATTTTCTTATGAAAATGAAGTCTGCAAAC
  AAGATTACTTTATTAAATCACCACCTTCTCAGCTGTTCTCCTCTGTGACCTCTTGGAAAAAGCGGTTTTT
  CATCCTGTCAAAGGCTGGGGAAAAGAGCTTTAGTCTTTCCTATTATAAAGACCATCATCACCGAGGTTCC
  ATTGAAATTGATCAAAATTCCAGTGTAGAAGTTGGCATAAGTAGCCAGGAAAAGATGCAATCTGTGCAGA
  AGATGTTTAAATGCCACCCTGATGAGGTCATGTCCATCAGAACCACTAACAGGGAATACTTCCTCATTGG
  CCACGACAGGGAGAAGATTAAAGACTGGGTCTCCTTCATGTCATCATTTCGCCAGGATATAAAAGCAACA
  CAGCAGAACACAGAGGAGGAACTCTCATTGGGTAATAAAAGAACCCTCTTCTACTCCAGCCCTCTCCTTG
  GCCCTTCCAGCACATCAGAGGCTGTTGGCTCCAGCTCACCAAGAAATGGTCTCCAAGACAAGCATTTAAT
  GGAACAAAGTTCTCCAGGATTTAGGCAAACTCACCTACAAGATTTATCAGAAGCCACTCAAGATGTGAAG
  GAAGAGAATCATTATCTTACTCCTCGAAGTGTTCTTTTAGAGTTGGATAATATCATTGCTTCCAGTGATT
  CTGGTGAATCCATTGAAACTGATGGTCCAGACCAGGTCTCTGGAAGAATTGAGTGTCATTATGAGCCAAT
  GGAATCCTATTTTTTCAAAGAGACATCCCATGAGTCTGTGGATAGCAGCAAAGAGGAACCCCAGACCCTT
  CCAGAGACCCAGGATGGGGACCTCCACCTGCAAGAACAAGGCTCAGGAATTGATTGGTGTCTTTCCCCTG
  CCGATGTGGAAGCACAGACCACAAATGACCAAAAGGGGTCGGCCTCACTAACTGTTGTGCAATTGTCTAT
  ATTAATCAATAATATCCCCGATGAAAGCCAAGTGGAGAAACTGAACGTTTTCCTTTCTCCTCCTGATGTC
  ATCAACTATCTTGCTCTCACAGAAGCCACAGGACGGATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212940 representing NM_024889
  Red=Cloning site Green=Tags(s)
  
  MEPKPQKSPGKQFTFSYENEVCKQDYFIKSPPSQLFSSVTSWKKRFFILSKAGEKSFSLSYYKDHHHRGS
  IEIDQNSSVEVGISSQEKMQSVQKMFKCHPDEVMSIRTTNREYFLIGHDREKIKDWVSFMSSFRQDIKAT
  QQNTEEELSLGNKRTLFYSSPLLGPSSTSEAVGSSSPRNGLQDKHLMEQSSPGFRQTHLQDLSEATQDVK
  EENHYLTPRSVLLELDNIIASSDSGESIETDGPDQVSGRIECHYEPMESYFFKETSHESVDSSKEEPQTL
  PETQDGDLHLQEQGSGIDWCLSPADVEAQTTNDQKGSASLTVVQLSILINNIPDESQVEKLNVFLSPPDV
  INYLALTEATGRI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024889
• ORF Size: 1089 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024889.3, NM_024889.4, NP_079165.3
• RefSeq Size: 3249
• RefSeq ORF: 1092
• Locus ID: 79949
• MW: 40.8 kDa