ALDH8A1 (NM_022568) Human Tagged ORF Clone

CAT#: RC213029

ALDH8A1 (Myc-DDK-tagged)-Human aldehyde dehydrogenase 8 family, member A1 (ALDH8A1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY411634) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213029 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ALDH8A1 protein (Cat# TP313029). The protein was produced from HEK293T cells transfected with ALDH8A1 cDNA clone (Cat# RC213029) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALDH8A1
• Synonyms: ALDH12; DJ352A20.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213029 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGAACAAACGCACTTTTGATGCTGGAAAACTTCATAGATGGAAAATTTTTACCTTGTAGCTCAT
  ATATAGATTCTTACGACCCATCAACAGGGGAAGTGTATTGCAGAGTGCCAAATAGTGGAAAAGACGAGAT
  CGAAGCCGCGGTCAAGGCCGCCAGAGAAGCCTTTCCCAGCTGGTCATCCCGCAGCCCCCAGGAGCGCTCA
  CGGGTCCTGAACCAGGTGGCGGATTTGCTGGAGCAGTCCCTGGAGGAGTTTGCCCAGGCCGAGTCTAAAG
  ACCAAGGGAAAACCTTAGCACTGGCAAGAACCATGGACATTCCCCGGTCTGTGCAGAACTTCAGGTTCTT
  CGCTTCCTCCAGCCTGCACCACACGTCAGAGTGCACGCAGATGGACCACCTGGGCTGCATGCACTACACG
  GTGCGGGCCCCGGTGGGAGTCGCTGGTCTGATCAGCCCCTGGAATTTGCCACTCTACTTGCTGACCTGGA
  AGATAGCTCCAGCGATGGCTGCAGGGAACACTGTGATAGCCAAGCCCAGTGAGCTGACTTCAGTGACTGC
  GTGGATGTTGTGCAAACTCCTGGATAAAGCAGGTGTTCCACCAGGTGTGGTCAATATTGTGTTTGGAACC
  GGGCCCAGGGTGGGTGAGGCCCTGGTGTCCCACCCAGAGGTGCCCCTGATCTCCTTCACCGGGAGCCAGC
  CCACCGCTGAGCGGATCACCCAGCTGAGCGCTCCCCACTGCAAAAAGCTCTCCCTGGAGCTGGGGGGCAA
  GAATCCTGCCATCATCTTTGAGGACGCCAACCTGGATGAGTGCATTCCGGCAACCGTCAGGTCCAGCTTT
  GCCAACCAGGGTGAAATCTGTCTCTGTACCAGCAGGATCTTTGTCCAGAAGAGCATCTATAGTGAATTTT
  TAAAGAGATTTGTAGAAGCTACCAGAAAGTGGAAAGTCGGCATTCCCTCTGATCCACTGGTGAGCATAGG
  TGCTCTGATAAGTAAAGCACATTTGGAGAAAGTCAGAAGTTACGTCAAGAGAGCTCTTGCTGAAGGTGCC
  CAAATTTGGTGCGGTGAGGGAGTGGATAAGTTGAGCCTCCCTGCCAGGAACCAGGCAGGCTACTTTATGC
  TTCCCACGGTGATAACAGACATTAAGGATGAATCCTGCTGCATGACGGAAGAGATATTTGGTCCAGTGAC
  GTGTGTCGTCCCCTTTGATAGTGAAGAGGAGGTGATTGAAAGAGCCAACAACGTTAAGTATGGGCTGGCG
  GCTACCGTGTGGTCCAGCAATGTGGGGCGCGTCCACCGGGTGGCTAAGAAGCTGCAGTCTGGCTTGGTCT
  GGACCAACTGCTGGCTCATCAGGGAGCTGAACCTTCCTTTCGGGGGGATGAAGAGTTCTGGAATAGGTAG
  AGAGGGAGCCAAGGACTCTTACGACTTCTTCACTGAGATCAAAACCATCACCGTTAAACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213029 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGTNALLMLENFIDGKFLPCSSYIDSYDPSTGEVYCRVPNSGKDEIEAAVKAAREAFPSWSSRSPQERS
  RVLNQVADLLEQSLEEFAQAESKDQGKTLALARTMDIPRSVQNFRFFASSSLHHTSECTQMDHLGCMHYT
  VRAPVGVAGLISPWNLPLYLLTWKIAPAMAAGNTVIAKPSELTSVTAWMLCKLLDKAGVPPGVVNIVFGT
  GPRVGEALVSHPEVPLISFTGSQPTAERITQLSAPHCKKLSLELGGKNPAIIFEDANLDECIPATVRSSF
  ANQGEICLCTSRIFVQKSIYSEFLKRFVEATRKWKVGIPSDPLVSIGALISKAHLEKVRSYVKRALAEGA
  QIWCGEGVDKLSLPARNQAGYFMLPTVITDIKDESCCMTEEIFGPVTCVVPFDSEEEVIERANNVKYGLA
  ATVWSSNVGRVHRVAKKLQSGLVWTNCWLIRELNLPFGGMKSSGIGREGAKDSYDFFTEIKTITVKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022568
• ORF Size: 1461 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022568.1, NM_022568.2, NM_022568.3, NP_072090.1
• RefSeq Size: 2567 bp
• RefSeq ORF: 1464 bp
• Locus ID: 64577
• Domains: aldedh
• Protein Families: Druggable Genome
• MW: 53.4 kDa
• Gene Summary: This gene encodes a member of the aldehyde dehydrogenase family of proteins. The encoded protein has been implicated in the synthesis of 9-cis-retinoic acid and in the breakdown of the amino acid tryptophan. This enzyme converts 9-cis-retinal into the retinoid X receptor ligand 9-cis-retinoic acid, and has approximately 40-fold higher activity with 9-cis-retinal than with all-trans-retinal. In addition, this enzyme has been shown to catalyze the conversion of 2-aminomuconic semialdehyde to 2-aminomuconate in the kynurenine pathway of tryptophan catabolism. [provided by RefSeq, Jul 2018]