Cytokeratin 2 (KRT2) (NM_000423) Human Tagged ORF Clone
CAT#: RC213030
- TrueORF®
KRT2 (Myc-DDK-tagged)-Human keratin 2 (KRT2)
"NM_000423" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | KRT2 |
Synonyms | CK-2e; K2e; KRT2A; KRT2E; KRTE |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC213030 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTGTCAGATCTCTTGCAAATCTCGAGGAAGAGGAGGAGGTGGAGGAGGATTCCGGGGCTTCAGCA GCGGCTCAGCTGTGGTGTCTGGTGGAAGCCGGAGATCAACTTCCAGCTTCTCCTGCTTGAGCCGCCATGG TGGTGGTGGCGGGGGCTTCGGTGGAGGCGGCTTTGGCAGTCGGAGTCTTGTTGGCCTTGGAGGGACCAAG AGCATCTCCATTAGTGTGGCTGGAGGAGGTGGTGGCTTTGGCGCCGCTGGTGGATTTGGTGGCAGAGGAG GTGGTTTTGGAGGCGGCAGCAGCTTTGGAGGTGGCAGCGGCTTCAGTGGTGGTGGTTTCGGTGGAGGCGG CTTTGGTGGAGGCCGCTTTGGAGGTTTTGGGGGCCCTGGTGGTGTTGGAGGTTTAGGGGGTCCTGGTGGC TTTGGGCCTGGAGGATACCCTGGTGGCATCCACGAAGTCTCTGTCAACCAGAGCCTCCTGCAGCCTCTCA ACGTGAAAGTTGACCCAGAGATCCAGAATGTGAAGGCCCAAGAGCGTGAGCAGATCAAAACTCTCAACAA CAAATTTGCCTCCTTCATTGACAAGGTGCGGTTCTTGGAGCAGCAGAACCAGGTGTTACAGACCAAATGG GAGCTGCTACAACAAATGAATGTTGGCACCCGCCCCATCAACCTGGAGCCCATCTTCCAGGGGTATATCG ACAGCCTCAAGAGATATCTGGATGGGCTCACTGCAGAAAGAACATCACAGAATTCAGAGCTGAATAACAT GCAGGATCTTGTGGAGGATTATAAGAAGAAGTATGAGGATGAAATCAATAAGCGCACAGCTGCTGAGAAT GATTTTGTGACGCTTAAAAAGGACGTGGACAATGCCTACATGATAAAGGTGGAGTTGCAGTCCAAGGTGG ACCTGCTGAACCAGGAAATTGAGTTTCTGAAAGTTCTCTATGATGCGGAGATATCCCAGATACATCAGAG TGTCACTGACACCAACGTCATCCTCTCCATGGACAACAGCCGCAACCTGGACTTGGATAGCATCATCGCC GAGGTCAAGGCCCAGTATGAGGAGATCGCCCAGAGGAGCAAGGAAGAAGCGGAGGCCCTGTACCACAGCA AGTATGAGGAGCTCCAGGTGACTGTCGGGAGACATGGAGACAGCCTGAAAGAGATCAAGATAGAGATCAG CGAGCTGAACCGCGTGATCCAGAGGCTGCAGGGGGAGATCGCACATGTGAAGAAGCAGTGTAAGAATGTG CAAGATGCCATCGCAGATGCCGAGCAGCGTGGGGAGCATGCCCTCAAGGATGCCAGGAACAAGTTGAATG ACCTGGAGGAGGCCCTGCAGCAGGCCAAGGAGGACTTGGCGCGGCTGCTGCGTGACTACCAGGAGCTGAT GAACGTGAAGCTGGCCCTAGATGTGGAGATCGCCACCTACCGCAAACTGCTGGAGGGCGAGGAGTGCAGG ATGTCTGGAGACCTCAGCAGCAATGTGACTGTGTCTGTGACAAGCAGCACCATTTCATCAAATGTGGCAT CCAAGGCTGCCTTTGGAGGTTCTGGAGGTAGAGGGTCCAGTTCCGGAGGAGGATACAGCTCTGGAAGCAG CAGTTATGGCTCTGGAGGCCGACAGTCTGGCTCCAGAGGCGGTAGTGGAGGAGGAGGTTCTATCTCTGGA GGAGGATATGGCTCTGGCGGTGGTTCTGGAGGAAGATACGGATCTGGTGGTGGCTCTAAGGGAGGGTCCA TCTCTGGAGGAGGATATGGCTCTGGAGGTGGAAAACACAGCTCTGGAGGTGGCTCTAGAGGAGGCTCCAG CTCTGGAGGAGGATATGGCTCTGGAGGTGGGGGTTCTAGCTCTGTAAAGGGTAGCTCAGGTGAAGCTTTT GGTTCCAGCGTGACCTTCTCTTTTAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213030 protein sequence
Red=Cloning site Green=Tags(s) MSCQISCKSRGRGGGGGGFRGFSSGSAVVSGGSRRSTSSFSCLSRHGGGGGGFGGGGFGSRSLVGLGGTK SISISVAGGGGGFGAAGGFGGRGGGFGGGSSFGGGSGFSGGGFGGGGFGGGRFGGFGGPGGVGGLGGPGG FGPGGYPGGIHEVSVNQSLLQPLNVKVDPEIQNVKAQEREQIKTLNNKFASFIDKVRFLEQQNQVLQTKW ELLQQMNVGTRPINLEPIFQGYIDSLKRYLDGLTAERTSQNSELNNMQDLVEDYKKKYEDEINKRTAAEN DFVTLKKDVDNAYMIKVELQSKVDLLNQEIEFLKVLYDAEISQIHQSVTDTNVILSMDNSRNLDLDSIIA EVKAQYEEIAQRSKEEAEALYHSKYEELQVTVGRHGDSLKEIKIEISELNRVIQRLQGEIAHVKKQCKNV QDAIADAEQRGEHALKDARNKLNDLEEALQQAKEDLARLLRDYQELMNVKLALDVEIATYRKLLEGEECR MSGDLSSNVTVSVTSSTISSNVASKAAFGGSGGRGSSSGGGYSSGSSSYGSGGRQSGSRGGSGGGGSISG GGYGSGGGSGGRYGSGGGSKGGSISGGGYGSGGGKHSSGGGSRGGSSSGGGYGSGGGGSSSVKGSSGEAF GSSVTFSFR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000423 |
ORF Size | 1917 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000423.2, NP_000414.2 |
RefSeq Size | 2459 bp |
RefSeq ORF | 1920 bp |
Locus ID | 3849 |
Cytogenetics | 12q13.13 |
MW | 65.4 kDa |
Gene Summary | 'The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC300067 | KRT2 (untagged)-Human keratin 2 (KRT2) |
USD 760.00 |
|
RG213030 | KRT2 (GFP-tagged) - Human keratin 2 (KRT2) |
USD 460.00 |
|
RC213030L3 | Lenti ORF clone of Human keratin 2 (KRT2), Myc-DDK-tagged |
USD 620.00 |
|
RC213030L4 | Lenti ORF clone of Human keratin 2 (KRT2), mGFP tagged |
USD 620.00 |
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