HAT1 (NM_003642) Human Tagged ORF Clone

CAT#: RC213094

HAT1 (Myc-DDK-tagged)-Human histone acetyltransferase 1 (HAT1), transcript variant 1. Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HAT1
• Synonyms: KAT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213094 ORF sequence, codon optimized.
  Due to the complexity of NM_003642, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCTTCGGCGCTATGGAGAAATTTCTCGTTGAATACAAGTCTGCCGTGGAGAAGAAATTGGCGG
  AGTACAAGTGCAATACCAACACTGCTATTGAACTCAAATTGGTTCGCTTCCCCGAAGACCTCGAAAATGA
  CATCAGGACATTTTTTCCAGAATATACTCATCAGCTGTTCGGGGACGATGAAACAGCCTTTGGTTATAAA
  GGCCTCAAAATTCTGCTCTACTACATTGCAGGAAGCTTGAGCACCATGTTCCGCGTAGAATATGCGAGTA
  AGGTGGATGAGAATTTCGACTGCGTCGAGGCCGACGATGTGGAAGGGAAGATACGGCAGATCATCCCTCC
  CGGCTTTTGTACTAATACCAATGACTTTCTTTCCCTGCTCGAAAAGGAGGTGGACTTCAAGCCTTTCGGC
  ACCCTGCTGCATACCTACAGTGTGCTTTCTCCTACTGGCGGGGAAAACTTCACGTTCCAAATCTACAAGG
  CCGACATGACTTGTAGAGGGTTTCGGGAGTACCATGAGAGGTTGCAGACTTTTCTCATGTGGTTCATCGA
  AACGGCCTCATTCATCGACGTGGATGATGAGCGCTGGCACTACTTTCTGGTATTTGAAAAGTACAATAAG
  GACGGCGCCACATTGTTTGCGACTGTGGGGTACATGACCGTGTACAATTACTACGTTTATCCGGACAAGA
  CACGCCCACGAGTAAGTCAGATGCTGATCCTTACCCCTTTCCAAGGGCAGGGACACGGCGCGCAATTGCT
  TGAAACTGTCCACCGCTACTATACCGAGTTCCCCACTGTGCTCGATATTACAGCTGAGGACCCCTCCAAG
  TCCTACGTGAAGCTGCGAGATTTTGTGTTGGTGAAACTGTGCCAAGATCTCCCCTGTTTCTCCCGAGAAA
  AGCTGATGCAGGGATTTAACGAGGACATGGCCATCGAAGCCCAGCAGAAGTTTAAAATTAACAAGCAGCA
  CGCTAGGCGAGTTTATGAAATTTTGAGGCTCTTGGTCACAGATATGAGCGACGCCGAACAGTATAGGTCT
  TACCGGCTCGACATAAAACGGCGGCTGATATCACCTTATAAGAAGAAACAGCGAGATCTGGCAAAAATGA
  GAAAATGTCTGAGGCCCGAGGAGCTTACTAACCAAATGAATCAGATCGAGATTTCAATGCAACATGAACA
  ACTGGAGGAGTCATTCCAAGAACTGGTCGAGGATTACAGGAGAGTCATCGAGCGGCTCGCCCAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213094 representing NM_003642
  Red=Cloning site Green=Tags(s)
  
  MAGFGAMEKFLVEYKSAVEKKLAEYKCNTNTAIELKLVRFPEDLENDIRTFFPEYTHQLFGDDETAFGYK
  GLKILLYYIAGSLSTMFRVEYASKVDENFDCVEADDVEGKIRQIIPPGFCTNTNDFLSLLEKEVDFKPFG
  TLLHTYSVLSPTGGENFTFQIYKADMTCRGFREYHERLQTFLMWFIETASFIDVDDERWHYFLVFEKYNK
  DGATLFATVGYMTVYNYYVYPDKTRPRVSQMLILTPFQGQGHGAQLLETVHRYYTEFPTVLDITAEDPSK
  SYVKLRDFVLVKLCQDLPCFSREKLMQGFNEDMAIEAQQKFKINKQHARRVYEILRLLVTDMSDAEQYRS
  YRLDIKRRLISPYKKKQRDLAKMRKCLRPEELTNQMNQIEISMQHEQLEESFQELVEDYRRVIERLAQE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003642
• ORF Size: 1257 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003642.2, NP_003633.1
• RefSeq Size: 1634
• RefSeq ORF: 1260
• Locus ID: 8520
• Protein Families: Druggable Genome
• MW: 49.5 kDa
• Gene Summary: The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jun 2009]