SEPTIN4 (NM_004574) Human Tagged ORF Clone

CAT#: RC213106

SEPT4 (Myc-DDK-tagged)-Human septin 4 (SEPT4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY401449) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213106 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SEPTIN4 protein (Cat# TP313106). The protein was produced from HEK293T cells transfected with SEPTIN4 cDNA clone (Cat# RC213106) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEPTIN4
• Synonyms: ARTS; BRADEION; C17orf47; CE5B3; H5; hCDCREL-2; hucep-7; MART; PNUTL2; SEP4; SEPT4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213106 representing NM_004574
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGTTCACTGGGATGGCAAGGGAATTCTGTCCCTGAGGACAGGACTGAAGCTGGGATCAAGCGTT
  TCCTGGAGGACACCACGGATGATGGAGAACTGAGCAAGTTCGTGAAGGATTTCTCAGGAAATGCGAGCTG
  CCACCCACCAGAGGCTAAGACCTGGGCATCCAGGCCCCAAGTCCCGGAGCCAAGGCCCCAGGCCCCGGAC
  CTCTATGATGATGACCTGGAGTTCAGACCCCCCTCGCGGCCCCAGTCCTCTGACAACCAGCAGTACTTCT
  GTGCCCCAGCCCCTCTCAGCCCATCTGCCAGGCCCCGCAGCCCATGGGGCAAGCTTGATCCCTATGATTC
  CTCTGAGGATGACAAGGAGTATGTGGGCTTTGCAACCCTCCCCAACCAAGTCCACCGAAAGTCCGTGAAG
  AAAGGCTTTGACTTTACCCTCATGGTGGCAGGAGAGTCTGGCCTGGGCAAATCCACACTTGTCAATAGCC
  TCTTCCTCACTGATCTGTACCGGGACCGGAAACTTCTTGGTGCTGAAGAGAGGATCATGCAAACTGTGGA
  GATCACTAAGCATGCAGTGGACATAGAAGAGAAGGGTGTGAGGCTGCGGCTCACCATTGTGGACACACCA
  GGTTTTGGGGATGCAGTCAACAACACAGAGTGCTGGAAGCCTGTGGCAGAATACATTGATCAGCAGTTTG
  AGCAGTATTTCCGAGACGAGAGTGGCCTGAACCGAAAGAACATCCAAGACAACAGGGTGCACTGCTGCCT
  GTACTTCATCTCACCCTTCGGCCATGGGCTCCGGCCATTGGATGTTGAATTCATGAAGGCCCTGCATCAG
  CGGGTCAACATCGTGCCTATCCTGGCTAAGGCAGACACACTGACACCTCCCGAAGTGGACCACAAGAAAC
  GCAAAATCCGGGAGGAGATTGAGCATTTTGGAATCAAGATCTATCAATTCCCAGACTGTGACTCTGATGA
  GGATGAGGACTTCAAATTGCAGGACCAAGCCCTAAAGGAAAGCATCCCATTTGCAGTAATTGGCAGCAAC
  ACTGTAGTAGAGGCCAGAGGGCGGCGAGTTCGGGGTCGACTCTACCCCTGGGGCATCGTGGAAGTGGAAA
  ACCCAGGGCACTGCGACTTTGTGAAGCTGAGGACAATGCTGGTACGTACCCACATGCAGGACCTGAAGGA
  TGTGACGCGGGAGACACATTATGAGAACTACCGGGCACAGTGCATCCAGAGCATGACCCGCCTGGTGGTG
  AAGGAACGGAATCGCAACAAACTGACTCGGGAAAGTGGTACCGACTTCCCCATCCCTGCTGTCCCACCAG
  GGACAGATCCAGAAACTGAGAAGCTTATCCGAGAGAAAGATGAGGAGCTGCGGCGGATGCAGGAGATGCT
  ACACAAAATACAAAAACAGATGAAGGAGAACTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213106 representing NM_004574
  Red=Cloning site Green=Tags(s)
  
  MDRSLGWQGNSVPEDRTEAGIKRFLEDTTDDGELSKFVKDFSGNASCHPPEAKTWASRPQVPEPRPQAPD
  LYDDDLEFRPPSRPQSSDNQQYFCAPAPLSPSARPRSPWGKLDPYDSSEDDKEYVGFATLPNQVHRKSVK
  KGFDFTLMVAGESGLGKSTLVNSLFLTDLYRDRKLLGAEERIMQTVEITKHAVDIEEKGVRLRLTIVDTP
  GFGDAVNNTECWKPVAEYIDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPLDVEFMKALHQ
  RVNIVPILAKADTLTPPEVDHKKRKIREEIEHFGIKIYQFPDCDSDEDEDFKLQDQALKESIPFAVIGSN
  TVVEARGRRVRGRLYPWGIVEVENPGHCDFVKLRTMLVRTHMQDLKDVTRETHYENYRAQCIQSMTRLVV
  KERNRNKLTRESGTDFPIPAVPPGTDPETEKLIREKDEELRRMQEMLHKIQKQMKENY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004574
• ORF Size: 1434 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004574.1, NM_004574.2, NM_004574.3, NM_004574.4, NP_004565.1
• RefSeq Size: 1767 bp
• RefSeq ORF: 1437 bp
• Locus ID: 5414
• Cytogenetics: 17q22
• Domains: GTP_CDC
• MW: 54.9 kDa
• Gene Summary: 'This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. [provided by RefSeq, Nov 2010]'