PIGQ (NM_004204) Human Tagged ORF Clone

CAT#: RC213194

PIGQ (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class Q (PIGQ), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIGQ
• Synonyms: c407A10.1; GPI1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213194 representing NM_004204
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTCAAGGCCTTCTTCCCCACGTGCTGCGTCTCGACGGACAGCGGGCTGCTGGTGGGACGGTGGG
  TGCCGGAGCAGAGCAGCGCCGTGGTCCTGGCGGTCCTGCACTTTCCCTTCATCCCCATCCAGGTCAAGCA
  GCTCCTGGCCCAGGTGCGGCAGGCCAGCCAGGTGGGCGTGGCCGTGCTGGGCACCTGGTGCCACTGCCGG
  CAGGAGCCCGAGGAGAGCCTGGGCCGCTTCCTGGAGAGCCTGGGTGCTGTCTTCCCCCATGAGCCCTGGC
  TGCGGCTGTGCCGGGAGAGAGGCGGCACGTTCTGGAGCTGCGAGGCCACCCACCGGCAAGCGCCCACTGC
  CCCCGGTGCCCCTGGTGAGGACCAGGTCATGCTCATCTTCTATGACCAGCGCCAGGTGTTGCTGTCACAG
  CTACACCTGCCCACCGTCCTGCCCGACCGCCAGGCTGGAGCCACCACTGCCAGCACGGGGGGCCTGGCTG
  CCGTCTTCGACACGGTAGCACGCAGTGAGGTGCTCTTCCGCAGTGACCGCTTTGATGAGGGCCCCGTGCG
  GCTGAGCCACTGGCAGTCGGAGGGCGTGGAGGCCAGCATCCTCGCGGAGCTGGCCAGGCGAGCCTCGGGA
  CCCATTTGCCTGCTGTTGGCCAGCCTGCTGTCGCTGGTCTCAGCTGTCAGTGCCTGCCGAGTGTTCAAGC
  TCTGGCCCCTGTCCTTCCTCGGGAGCAAACTCTCCACGTGCGAACAGCTCCGGCACCGGCTGGAGCACCT
  CACGCTAATCTTCAGTACACGGAAGGCGGAGAACCCTGCCCAGCTGATGAGGAAGGCCAACACGGTGGCC
  TCTGTGCTGCTGGACGTGGCCCTGGGCCTCATGCTGCTGTCCTGGCTCCACGGGAGAAGCCGCATCGGGC
  ATCTGGCCGACGCCCTCGTTCCTGTGGCTGACCACGTGGCCGAGGAGCTCCAGCATCTGCTGCAGTGGCT
  GATGGGTGCTCCCGCCGGGCTCAAGATGAACCGTGCACTGGACCAGGTGCTGGGCCGCTTCTTCCTCTAC
  CACATCCACCTGTGGATCAGCTACATCCACCTCATGTCCCCCTTCGTGGAGCACATCCTTTGGCACGTGG
  GCCTCTCGGCCTGCCTGGGCCTGACGGTGGCCCTGTCCCTCCTCTCGGACATTATCGCCCTCCTCACCTT
  CCACATCTACTGCTTTTACGTCTATGGAGCCAGGCTGTACTGCCTGAAGATCCATGGCCTGTCCTCACTG
  TGGCGTCTGTTCCGGGGGAAGAAGTGGAACGTTCTGCGCCAGCGCGTGGACTCCTGTTCCTATGACCTGG
  ACCAGCTGTTCATCGGGACTCTGCTCTTCACCATCCTGCTCTTCCTCCTGCCTACCACAGCCCTGTACTA
  CCTGGTGTTCACCCTGCTCCGGCTCCTGGTGGTCGCCGTGCAGGGCCTGATCCATCTGCTCGTGGACCTC
  ATCAACTCCCTGCCGCTGTACTCACTGGGTCTTCGGCTCTGCCGGCCCTACAGGCTGGCGGCTGGCGTGA
  AGTTCCGTGTCCTCCGGCACGAGGCCGGCAGGCCCCTCCGCCTCCTGATGCAGATAAACCCACTGCCCTA
  CAGCCGCGTGGTGCACACCTACCGCCTCCCCAGCTGTGGCTGCCACCCCAAGCACTCCTGGGGCGCCCTG
  TGCCGCAAGCTGTTCCTTGGGGAGCTCATCTACCCCTGGAGGCAGAGAGGGGACAAGCAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213194 representing NM_004204
  Red=Cloning site Green=Tags(s)
  
  MVLKAFFPTCCVSTDSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGVAVLGTWCHCR
  QEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGAPGEDQVMLIFYDQRQVLLSQ
  LHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDRFDEGPVRLSHWQSEGVEASILAELARRASG
  PICLLLASLLSLVSAVSACRVFKLWPLSFLGSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVA
  SVLLDVALGLMLLSWLHGRSRIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLY
  HIHLWISYIHLMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL
  WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAVQGLIHLLVDL
  INSLPLYSLGLRLCRPYRLAAGVKFRVLRHEAGRPLRLLMQINPLPYSRVVHTYRLPSCGCHPKHSWGAL
  CRKLFLGELIYPWRQRGDKQD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004204
• ORF Size: 1743 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004204.1, NM_004204.2, NM_004204.3, NP_004195.2
• RefSeq Size: 2921
• RefSeq ORF: 1746
• Locus ID: 9091
• Domains: Gpi1
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• MW: 65.2 kDa
• Gene Summary: This gene is involved in the first step in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes a N-acetylglucosaminyl transferase component that is part of the complex that catalyzes transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]