CYP4Z1 (NM_178134) Human Tagged ORF Clone

CAT#: RC213223

CYP4Z1 (Myc-DDK-tagged)-Human cytochrome P450, family 4, subfamily Z, polypeptide 1 (CYP4Z1)

Product Images

Western blot validation of overexpression lysate (Cat# LY405940) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213223 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CYP4Z1 protein (Cat# TP313223). The protein was produced from HEK293T cells transfected with CYP4Z1 cDNA clone (Cat# RC213223) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CYP4Z1
• Synonyms: CYP4A20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213223 representing NM_178134
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCTCCTGGCTTCAGGAACTCATGGCTCACCCCTTCTTGCTGCTGATCCTCCTCTGCATGTCTC
  TGCTGCTGTTTCAGGTAATCAGGTTGTACCAGAGGAGGAGATGGATGATCAGAGCCCTGCACCTGTTTCC
  TGCACCCCCTGCCCACTGGTTCTATGGCCACAAGGAGTTTTACCCAGTAAAGGAGTTTGAGGTGTATCAT
  AAGCTGATGGAAAAATACCCATGTGCTGTTCCCTTGTGGGTTGGACCCTTTACGATGTTCTTCAGTGTCC
  ATGACCCAGACTATGCCAAGATTCTCCTGAAAAGACAAGATCCCAAAAGTGCTGTTAGCCACAAAATCCT
  TGAATCCTGGGTTGGTCGAGGACTTGTGACCCTGGATGGTTCTAAATGGAAAAAGCACCGCCAGATTGTG
  AAACCTGGCTTCAACATCAGCATTCTGAAAATATTCATCACCATGATGTCTGAGAGTGTTCGGATGATGC
  TGAACAAATGGGAGGAACACATTGCCCAAAACTCACGTCTGGAGCTCTTTCAACATGTCTCCCTGATGAC
  CCTGGACAGCATCATGAAGTGTGCCTTCAGCCACCAGGGCAGCATCCAGTTGGACAGTACCCTGGACTCA
  TACCTGAAAGCAGTGTTCAACCTTAGCAAAATCTCCAACCAGCGCATGAACAATTTTCTACATCACAACG
  ACCTGGTTTTCAAATTCAGCTCTCAAGGCCAAATCTTTTCTAAATTTAACCAAGAACTTCATCAGTTCAC
  AGAGAAAGTAATCCAGGACCGGAAGGAGTCTCTTAAGGATAAGCTAAAACAAGATACTACTCAGAAAAGG
  CGCTGGGATTTTCTGGACATACTTTTGAGTGCCAAAAGCGAAAACACCAAAGATTTCTCTGAAGCAGATC
  TCCAGGCTGAAGTGAAAACGTTCATGTTTGCAGGACATGACACCACATCCAGTGCTATCTCCTGGATCCT
  TTACTGCTTGGCAAAGTACCCTGAGCATCAGCAGAGATGCCGAGATGAAATCAGGGAACTCCTAGGGGAT
  GGGTCTTCTATTACCTGGGAACACCTGAGCCAGATGCCTTACACCACGATGTGCATCAAGGAATGCCTCC
  GCCTCTACGCACCGGTAGTAAACATATCCCGGTTACTCGACAAACCCATTACTTTTCCAGATGGACGCTC
  CTTACCTGCAGGAATAACTGTGTTTATCAATATTTGGGCTCTTCACCACAACCCCTATTTCTGGGAAGAC
  CCTCAGGTCTTTAACCCCTTGAGATTCTCCAGGGAAAATTCTGAAAAAATACATCCCTATGCCTTCATAC
  CATTCTCAGCTGGATTAAGGAACTGCATTGGGCAGCATTTTGCCATAATTGAGTGTAAAGTGGCAGTGGC
  ATTAACTCTGCTCCGCTTCAAGCTGGCTCCAGACCACTCAAGGCCTCCCCAGCCTGTTCGTCAAGTTGTC
  CTCAAGTCCAAGAATGGAATCCATGTGTTTGCAAAAAAAGTTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213223 representing NM_178134
  Red=Cloning site Green=Tags(s)
  
  MEPSWLQELMAHPFLLLILLCMSLLLFQVIRLYQRRRWMIRALHLFPAPPAHWFYGHKEFYPVKEFEVYH
  KLMEKYPCAVPLWVGPFTMFFSVHDPDYAKILLKRQDPKSAVSHKILESWVGRGLVTLDGSKWKKHRQIV
  KPGFNISILKIFITMMSESVRMMLNKWEEHIAQNSRLELFQHVSLMTLDSIMKCAFSHQGSIQLDSTLDS
  YLKAVFNLSKISNQRMNNFLHHNDLVFKFSSQGQIFSKFNQELHQFTEKVIQDRKESLKDKLKQDTTQKR
  RWDFLDILLSAKSENTKDFSEADLQAEVKTFMFAGHDTTSSAISWILYCLAKYPEHQQRCRDEIRELLGD
  GSSITWEHLSQMPYTTMCIKECLRLYAPVVNISRLLDKPITFPDGRSLPAGITVFINIWALHHNPYFWED
  PQVFNPLRFSRENSEKIHPYAFIPFSAGLRNCIGQHFAIIECKVAVALTLLRFKLAPDHSRPPQPVRQVV
  LKSKNGIHVFAKKVC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178134
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178134.1, NM_178134.2, NP_835235.1
• RefSeq Size: 1907 bp
• RefSeq ORF: 1518 bp
• Locus ID: 199974
• Protein Families: Druggable Genome, P450, Transmembrane
• MW: 58.9 kDa
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This gene is part of a cluster of cytochrome P450 genes on chromosome 1p33. [provided by RefSeq, Jul 2008]