CRYBG2 (NM_001039775) Human Tagged ORF Clone

CAT#: RC213227

AIM1L (Myc-DDK-tagged)-Human absent in melanoma 1-like (AIM1L)

Product Images

Coomassie blue staining of purified CRYBG2 protein (Cat# TP313227). The protein was produced from HEK293T cells transfected with CRYBG2 cDNA clone (Cat# RC213227) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRYBG2
• Synonyms: AIM1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213227 representing NM_001039775
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGCAGGTGGGCCCATGGCCCGGGCCAAGGCCCGAGTGGTAAGTGCCACATTGACATGGCGGC
  AGCGGCCCCCCACCCAGGAAGAGATCAAACATGGTTTTCACAAGGTGTCCCTGGTGTCAGGGGCCCAGAT
  GGAAGCCCCGCAGAAGGAGATGTTTGAGTTCAGCCGTCGAGAGGAAGTGGAAGTCAATGGCTTTGCAACA
  CAGGAAGAAGAGACTGTGAATTGCCAGGGCCCTCGGGATACAGCTGGCTCCAAGAACTTCCAGAGCCATG
  GACCCATCTTTTCCAAGAAGTACATACCACCTCCCAAGGAGAAAAGGCCTGAGGGGAGGCTGAAGGAGGC
  TGTGGACCAGAGTGATGGCAGCCGCCAAGCTCCCAGGACTGAGCCCCCATGTGTGGGAGCTATGGCCAGG
  ACTGAGCTTTTGGTTCCCCTGCCTGGGCCCCGAGAGCCAAGTCCCCACCCAGGTGTAGGTCTCACCAGTG
  GTAGCTCCCGGAGCCTCGAGGAATACCGAGTGACACGCACTGTGCGGACCACCACAGTGGTGGGAGGTCA
  TGTGGACCGGCGGATGAGCAGCTCTGTGACTGTGAGGCCAGTGTCCTCAGGCGAGGCCCTGCCACGGGGC
  CGTCAGGTCTCCCGCATGGTGCCGCCAGTGGTGGTGGGCTCCCCACCAGGCTCGCCCAGCCGCAGCCAGG
  CCGTGAAAGTGCTAAGTAACCTCGTGCCTGCTGGGCACAGCCCCCCTGCCAGTCACCTGCCCAGGCCCAC
  GGCTGGCGGGCCAAGGAGCACAGGTCTGGGCAGCACAGTGGGGGCAGCCTTGAGGCAGCTGCCTGAGACC
  GGGACAGCAGAGCTTAAAGACAGCTCTGCCCTGGCCTCTACAGGCATCCCAGCCAGTGCTCACCTGCCTA
  AGAATCAGGATGCCCCTGCAGCCTGTCCGGACAGAGACCAGGGCAGAGCCCCGGATGCCAGGGCCTGTGA
  GCTCTGGCAGGTGCTGGGAGCCCCCAGTTCCACTGAGCTCCCTCTCCAGACTTCTCAGGGTCAAGCATCA
  GTCCCCTCCTCTCCCAGACTCGAGACCCATGTCCCCTCTCCTGGCCTAACTCACCCTGCAAAGCAGCCTG
  TGGTGCCCACTCACCCCGGGGCCCGGCTCACTCCCCTTGTTCTGCCCCCTAAAAAAAAGGACGGGCCCGT
  GGACCCCCCTGCTGCCACCGTCCTGCCCATGGTGAGGAGCGAGCATGTGACAGTCCCTGGACAACCTCCT
  GCTCCATCCACTACAAGAAGGAAGGATGTCCCCAGCCCAGGAGGTCTTTCTGCTCCATCGTCCCCAAGGA
  ATAAGTTTGTTCAGAACTCTGAAAATGTCCCTGTCCTCCCCTTTACCCAGAGGGAGGTCGTGAAGGGCCC
  CGGTGCTCCTGCTGCCTCATCTCCCACCCGGAAGGAGGTGGTGCAGGGGTCCAGTGCTTCTGCTGCCTCG
  TCCCCCACCTGGAAAGAGGTCGTGAAGGGCCCTGGTGCTCCTGCTGCCTCATCTCCCACCCAGAAGGAGG
  TGGTGCAGGGGTCCAGTGCTCCTGCTGCCTTGTTCCCCACCTGGAAAGAGGTCGTGAAGGGCCCCGGTGC
  TCCTGATGCCTCATTTCCCACCTGGAAAGAGGTTGTGAAGGGCCCTGGTGCTCCTGCTGCCTCATCCCCC
  ACCCAGAAGGAGGTGGTGCAGGGGTCTGGTGCTCCTGCTGCCTTGTCCACCACCCCAAAAGAGGTTGTGA
  AGGGCCCTGGTGCTCCTGCTGCCTCATCGCCCACCCAGAAAGAGGTTGTGAAGGGCCCCTGTGCTCCTGC
  TGCCTCATCTCCCACCCAGAAGGAGGTGGTGCAGGGGTCTGGTGCTCCTGCTGCCTTGTCCCCCAAGTCA
  ACTGAGGTTGTCCAGGGCCCAAAAGGCAGCAGTAGCATCCAAAAAGAGGCTGTCCAGGGTATTGCAGGCA
  GCCTTGCCCCGCCCCTCACCAAGGAAGAGACTGTTCAAGGCCCAATTGCTCCTGCCACCTCACTCCCCAA
  ACAGGATAAGGGGGTCCAGGACTCTGAAGGGAGCCCCATCTCATCTCTCACCCAGAAAGAGGTTGTGCAG
  GACCCTGATGCTCTCCCTGCCCCATCTTCCTCAGTGGACAGGGTGTCCCCCAGCCCAGGAGGCACCCCTG
  CCCCAGTGCCAACAGGAGCAGAGGCCAGCACGGAGTCCCAGCTTGTCTCTGATCCCACCGAGGGCAAGAC
  GTGCACAGAGACATCAAGGGAGGAGGATGAGGTGGCCCTGGCCGCTGACCTGGAGATATTCCTGGATACG
  CTGCGGAGCATGGAGCCCCCTGAGATCCTCCGCACTCACCGGCTGCCACGAGCCCCTCGCTCCTCCTACC
  TGTCCATGTACGCCACGCTGCCTGCCATTGAGGAGGACCAGCTGGGGCCATGGGTGCTGGGCCCCGGACC
  CCAGGAGGTGCCTTCACTGGAAGAGAAAGAGGAGGAGGAGGAGGAGGAACCAGAGAACCCCTATCTGAGT
  GACGATGAGAAGCTCCAGCGCAGGCAGGAGAAAGCTGGGCCCAGCCCCTCCAGGGACCTCCACCCTGCCA
  GACCCACCCAGGTCTCCTGCTCTCCTCTGGAGATGATGAAGAAGCATGTAGCAGGAACCAAGGGCCCCCA
  CTCAGAGCTGGGATTGGAACTGCAGGGAGGCAGCAGGCCCACTTCCCGTCTTGGAGGCAGCCTTCTGTTC
  GGCAGTCTGGTGCCTACCGCCAAGGAGGCCTCCACCCCGGAACCGCTGGGCACAAAACTATCTGCTCTGC
  TGCCCCATGGGGCACCGGGGCTCAGGAAGGTGCCAGGACAGTTGCCCCTGCTTTGCAGTGAAAGATCATC
  CCCAACGGAGAAGCTTGCCTGTTCCCTGCCCCTGGAGGGGTGGAGCCCAGCCTTAAAGACCCAGGGCAAG
  CTGAACACCAGGCCTGGAAAGGTGATCTTCTTCTCAGAGTCTGGCTGCCAAGGCAGTGGCAGGGAGGTCT
  GGGGAGACATCGTTGATGCCTCAGGCTGGGCCCCCGTAGCCTCCATAAGGGTAGTTCGAGGCTGCTGGGT
  GCTGTACGAAGAGCCAGAGTTCCGGGGTCAGAAGCTGGTCCTGCCTGAAGGAGACATGGAACTCAGAACC
  CCAGGGACAAAGTGGAGTCCCCAAGGCATCGGCTCCCTAAGGAGGGTTGTCTGGGACTACAGCACCCCGG
  AAATCAGCCTCTTCTCTGAGGAGGGCCTCAAGGGGGAGCAAGTGAAGCTAACAGAGGCCTTGAAGAATTC
  CCAGGGTCTGGAGAAGCCCCTGCAGGTGGCATCTGCCACCGTCTCTGCAGGACTGTGGCTACTGTACCCC
  AAACCATTATTCGAAGACACTCCCTACATCCTGGAACCTGGAGAGTACCCCACCTCAGAGGCCTGGGGCA
  CATCGGACCCCAGCGTGGGCTCCCTGAAGCCCATGAGATTGGGCTGCCCAAGTGTGGAGAAGCCAGGGGA
  GCCCAGGGCTGTGGTGTATGAGGCCCCAGGCTTTCAGGGCCGCAGCTGGGAAGTGAGCCGAGACATCTAC
  AACCTTCAGCAGCCAGAGGACAGCCAGAGCCCCCACCTGGCCTCTGTGGGGTCCCTGAGAGTTCTCGGAG
  GCTGCTGGGTGGGCTACGAGAAGGAGGGCTTCCGGGGCCACCAGTATCTGCTGGAGGAGGGGGAATACCC
  AGACTGGTCACACTGGGGAGGCTATGACGAGTTGCTGACCTCCCTCCGGGTCATCCGGACGGACTTCGGG
  GACCCGGCCGTCGTGCTATTTGAGGCCATGGACTTCGAGGGGCACGGCGTGGAGGTGAGCAAGGCATTGC
  CGGATGTGGAGCTGGTGCAACACGGCCCCAGCACACAGGCCATCCACGTGCTCAGCGGCGTGTGGGTGGC
  CTACCAGGAGGTGGGCTTCTCCGGGGAACAGTACGTGCTGGAGAAGGGCGTGTATCGTAACTGCGAGGAC
  TGGGGCGCTGGCAACAGCACCCTCGCCTCGCTGCAGCCGGTCCTACAGGTCGGGGAGCACGATCTGCACT
  TCGTCTCAAAGATTCAGCTTTTCTCCCGCCCCGACTTTCTGGGCGACCACTTCTCTTTCGAAGATGACCA
  GGCCGCTCTGCCCGCCTCCTTCCGACCTCAGTCCTGCCGGGTCCACGGCGGCAGCTGGATCCTGTTTGAT
  GAGACGAACTTCGAGGGTGACCAGCACATTCTCTCTGAGGGCGAGTTCCCCACTCTCACGGCCATGGGCT
  GCCTCGCCTCCACAGTCCTGGGCTCTCTCCAGAAGGTATCCCTGCACTTTTCAGAGCCTTCCATTTTCCT
  GTATGGACTCGAGTGCTTCGAGGGGAAGGAGATCGAGCTCAGCAGGGAGGTGCGGAGCCTGCAAGCCGAG
  GGCTTCAACAACCATGTGCTGTCTGTGCGGATCAAGGGGGGCATTTGGGTGCTATGTGAACACAGTGACT
  TCCGGGGCCGCCAGTGGCTGGTGGGAAGCTGCGAGATCACCAACTGGCTGACCTACAGCGGCACCCAGAG
  GGTGGGCTCCCTCTACCCCATCAAGCAGCGCCGGGTTTATTTCCGCCTCTGGAATGCAGCACTGGGGGGA
  TTCCTGGCAGTGCCGGACCATGTGGAGGACATGAAAGCAGGCCGTGTGGTGGTCGCCGACCCCCAAGCTG
  GAGGTAGCTGCATCTGGTACTACGAGGATGGGCTGCTGAAGAACCAGATGGCCCCCACCATGAGCCTACA
  GGTGATTGGACCCCCTAGCCCAGGCTCCAAGGTGGTGCTGTGGGCCGAGAGCCGCCTGCCGCGCCAGACG
  TGGAGCATCAGTGAATCGGGCCACATCTGCAGCCAGATGTTCGAAGGCCAGATCCTGGACGTGAAGGGAG
  GCCGGGGCTACGACCGGGACCACGTGGTGCTATGGGAGCCGGATGAGGACAGGGCATCCCAGATCTGGAC
  TATCCACGTGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213227 representing NM_001039775
  Red=Cloning site Green=Tags(s)
  
  MEEAGGPMARAKARVVSATLTWRQRPPTQEEIKHGFHKVSLVSGAQMEAPQKEMFEFSRREEVEVNGFAT
  QEEETVNCQGPRDTAGSKNFQSHGPIFSKKYIPPPKEKRPEGRLKEAVDQSDGSRQAPRTEPPCVGAMAR
  TELLVPLPGPREPSPHPGVGLTSGSSRSLEEYRVTRTVRTTTVVGGHVDRRMSSSVTVRPVSSGEALPRG
  RQVSRMVPPVVVGSPPGSPSRSQAVKVLSNLVPAGHSPPASHLPRPTAGGPRSTGLGSTVGAALRQLPET
  GTAELKDSSALASTGIPASAHLPKNQDAPAACPDRDQGRAPDARACELWQVLGAPSSTELPLQTSQGQAS
  VPSSPRLETHVPSPGLTHPAKQPVVPTHPGARLTPLVLPPKKKDGPVDPPAATVLPMVRSEHVTVPGQPP
  APSTTRRKDVPSPGGLSAPSSPRNKFVQNSENVPVLPFTQREVVKGPGAPAASSPTRKEVVQGSSASAAS
  SPTWKEVVKGPGAPAASSPTQKEVVQGSSAPAALFPTWKEVVKGPGAPDASFPTWKEVVKGPGAPAASSP
  TQKEVVQGSGAPAALSTTPKEVVKGPGAPAASSPTQKEVVKGPCAPAASSPTQKEVVQGSGAPAALSPKS
  TEVVQGPKGSSSIQKEAVQGIAGSLAPPLTKEETVQGPIAPATSLPKQDKGVQDSEGSPISSLTQKEVVQ
  DPDALPAPSSSVDRVSPSPGGTPAPVPTGAEASTESQLVSDPTEGKTCTETSREEDEVALAADLEIFLDT
  LRSMEPPEILRTHRLPRAPRSSYLSMYATLPAIEEDQLGPWVLGPGPQEVPSLEEKEEEEEEEPENPYLS
  DDEKLQRRQEKAGPSPSRDLHPARPTQVSCSPLEMMKKHVAGTKGPHSELGLELQGGSRPTSRLGGSLLF
  GSLVPTAKEASTPEPLGTKLSALLPHGAPGLRKVPGQLPLLCSERSSPTEKLACSLPLEGWSPALKTQGK
  LNTRPGKVIFFSESGCQGSGREVWGDIVDASGWAPVASIRVVRGCWVLYEEPEFRGQKLVLPEGDMELRT
  PGTKWSPQGIGSLRRVVWDYSTPEISLFSEEGLKGEQVKLTEALKNSQGLEKPLQVASATVSAGLWLLYP
  KPLFEDTPYILEPGEYPTSEAWGTSDPSVGSLKPMRLGCPSVEKPGEPRAVVYEAPGFQGRSWEVSRDIY
  NLQQPEDSQSPHLASVGSLRVLGGCWVGYEKEGFRGHQYLLEEGEYPDWSHWGGYDELLTSLRVIRTDFG
  DPAVVLFEAMDFEGHGVEVSKALPDVELVQHGPSTQAIHVLSGVWVAYQEVGFSGEQYVLEKGVYRNCED
  WGAGNSTLASLQPVLQVGEHDLHFVSKIQLFSRPDFLGDHFSFEDDQAALPASFRPQSCRVHGGSWILFD
  ETNFEGDQHILSEGEFPTLTAMGCLASTVLGSLQKVSLHFSEPSIFLYGLECFEGKEIELSREVRSLQAE
  GFNNHVLSVRIKGGIWVLCEHSDFRGRQWLVGSCEITNWLTYSGTQRVGSLYPIKQRRVYFRLWNAALGG
  FLAVPDHVEDMKAGRVVVADPQAGGSCIWYYEDGLLKNQMAPTMSLQVIGPPSPGSKVVLWAESRLPRQT
  WSISESGHICSQMFEGQILDVKGGRGYDRDHVVLWEPDEDRASQIWTIHVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039775
• ORF Size: 4983 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039775.3, NP_001034864.2
• RefSeq Size: 5245
• RefSeq ORF: 4986
• Locus ID: 55057
• MW: 178.4 kDa