CPEB1 (NM_001079533) Human Tagged ORF Clone

CAT#: RC213281

CPEB1 (Myc-DDK-tagged)-Human cytoplasmic polyadenylation element binding protein 1 (CPEB1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY421521) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213281 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPEB1
• Synonyms: CPE-BP1; CPEB; CPEB-1; h-CPEB; hCPEB-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213281 representing NM_001079533
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTTCCCAACCTCTGCGCAAGAATCTTCCCGTGGCCTCCCAGATGCAAATGACTTGTGCCTTGGCC
  TGCAGTCCCTCAGTCTGACAGGCTGGGACCGACCCTGGAGCACCCAGGACTCAGATTCCTCAGCCCAGAG
  CAGCACACACTCGGTACTGAGCATGCTCCATAACCCACTGGGAAATGTCCTAGGAAAACCCCCCTTGAGC
  TTCCTGCCTCTGGATCCCCTTGGGTCTGACTTGGTGGACAAGTTTCCAGCACCCTCAGTTAGAGGATCAC
  GCCTGGACACCCGGCCCATCCTGGACTCTCGATCTAGCAGCCCCTCTGACTCAGACACCAGTGGCTTCAG
  CTCTGGATCAGATCATCTCTCAGATTTGATTTCAAGCCTTCGCATTTCTCCACCTCTGCCCTTCCTGTCT
  CTGTCAGGGGGTGGTCCCAGAGACCCTTTAAAGATGGGGGTAGGGTCTCGGATGGACCAAGAGCAAGCTG
  CTCTTGCTGCAGTCACTCCCTCCCCAACCAGTGCTTCAAAGAGATGGCCAGGAGCTTCTGTGTGGCCATC
  CTGGGACCTCCTCGAAGCTCCCAAAGACCCCTTCAGCATAGAGAGAGAGGCCAGGCTGCACCGACAAGCT
  GCAGCTGTGAATGAAGCCACCTGTACCTGGAGTGGCCAGCTTCCTCCCCGGAACTATAAGAACCCCATCT
  ACTCTTGCAAGGTGTTTCTAGGAGGTGTTCCTTGGGATATTACAGAAGCTGGATTAGTTAACACCTTCCG
  TGTTTTTGGCTCTTTGAGTGTGGAGTGGCCTGGTAAGGATGGCAAGCATCCCCGGTGTCCTCCCAAAGGT
  AATATGCCTAAAGGGTATGTGTATCTGGTCTTCGAACTAGAGAAGTCTGTCCGATCCTTGCTTCAGGCTT
  GCTCTCATGACCCGCTGAGCCCAGATGGCCTGAGTGAATATTATTTCAAGATGTCCAGCCGAAGGATGCG
  CTGCAAGGAGGTGCAGGTGATCCCCTGGGTATTAGCCGACAGTAACTTTGTCCGGAGCCCATCTCAGAGG
  CTTGACCCCAGCAGGACGGTGTTTGTCGGTGCTCTGCATGGAATGCTAAATGCTGAGGCCCTGGCAGCCA
  TCTTGAACGACCTATTTGGTGGAGTGGTGTATGCCGGGATTGACACAGATAAGCACAAGTATCCCATTGG
  TTCTGGTCGTGTGACTTTCAATAACCAACGGAGTTACCTGAAAGCAGTCAGCGCTGCTTTTGTGGAGATC
  AAAACCACCAAGTTCACAAAGAAGGTTCAGATTGACCCCTACCTAGAAGATTCTCTGTGTCATATCTGCA
  GTTCTCAGCCTGGTCCTTTCTTCTGTCGAGATCAGGTCTGCTTCAAATACTTCTGCCGGAGCTGCTGGCA
  CTGGCGGCACAGCATGGAGGGCCTGCGCCACCACAGCCCCCTGATGCGGAACCAGAAGAACCGAGATTCC
  AGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213281 representing NM_001079533
  Red=Cloning site Green=Tags(s)
  
  MLFPTSAQESSRGLPDANDLCLGLQSLSLTGWDRPWSTQDSDSSAQSSTHSVLSMLHNPLGNVLGKPPLS
  FLPLDPLGSDLVDKFPAPSVRGSRLDTRPILDSRSSSPSDSDTSGFSSGSDHLSDLISSLRISPPLPFLS
  LSGGGPRDPLKMGVGSRMDQEQAALAAVTPSPTSASKRWPGASVWPSWDLLEAPKDPFSIEREARLHRQA
  AAVNEATCTWSGQLPPRNYKNPIYSCKVFLGGVPWDITEAGLVNTFRVFGSLSVEWPGKDGKHPRCPPKG
  NMPKGYVYLVFELEKSVRSLLQACSHDPLSPDGLSEYYFKMSSRRMRCKEVQVIPWVLADSNFVRSPSQR
  LDPSRTVFVGALHGMLNAEALAAILNDLFGGVVYAGIDTDKHKYPIGSGRVTFNNQRSYLKAVSAAFVEI
  KTTKFTKKVQIDPYLEDSLCHICSSQPGPFFCRDQVCFKYFCRSCWHWRHSMEGLRHHSPLMRNQKNRDS
  S
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079533
• ORF Size: 1473 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001079533.1, NP_001073001.1
• RefSeq Size: 3110
• RefSeq ORF: 1476
• Locus ID: 64506
• Protein Pathways: Dorso-ventral axis formation, Oocyte meiosis, Progesterone-mediated oocyte maturation
• MW: 54 kDa
• Gene Summary: This gene encodes a member of the cytoplasmic polyadenylation element binding protein family. This highly conserved protein binds to a specific RNA sequence, called the cytoplasmic polyadenylation element, found in the 3' untranslated region of some mRNAs. The encoded protein functions in both the cytoplasm and the nucleus. It is involved in the regulation of mRNA translation, as well as processing of the 3' untranslated region, and may play a role in cell proliferation and tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]