NMBR (NM_002511) Human Tagged ORF Clone

CAT#: RC213319

NMBR (Myc-DDK-tagged)-Human neuromedin B receptor (NMBR)

Product Images

Western blot validation of overexpression lysate (Cat# LY400899) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213319 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NMBR
• Synonyms: BB1; BB1R; NMB-R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213319 representing NM_002511
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCTAAGTCTCTTTCCAACCTCTCGGTGACCACCGGCGCGAATGAGAGCGGTTCCGTTCCCGAGG
  GGTGGGAAAGGGATTTCCTGCCGGCCTCGGACGGGACCACCACGGAGTTGGTGATCCGCTGTGTGATCCC
  GTCCCTCTACCTGCTCATCATCACCGTGGGCTTGCTGGGCAACATCATGCTGGTGAAGATCTTCATCACC
  AACAGCGCCATGAGGAGCGTCCCCAACATCTTCATCTCTAACCTGGCGGCCGGGGACTTGCTGCTGCTGC
  TCACCTGCGTCCCGGTGGACGCCTCGCGCTACTTCTTCGACGAGTGGATGTTTGGCAAGGTGGGCTGCAA
  ACTGATCCCTGTCATCCAGCTCACTTCCGTGGGGGTTTCCGTGTTCACTCTCACTGCCCTCAGCGCCGAC
  AGGTACAGAGCCATCGTTAACCCCATGGACATGCAGACGTCAGGGGCATTGCTGCGGACCTGTGTGAAGG
  CCATGGGTATCTGGGTGGTCTCCGTGTTGCTGGCAGTTCCCGAAGCGGTGTTTTCAGAAGTGGCTCGCAT
  CAGTAGCTTGGATAATAGCAGCTTCACAGCATGTATCCCATACCCTCAAACAGATGAATTACATCCAAAG
  ATTCATTCAGTGCTCATTTTCTTGGTCTATTTCCTCATACCACTTGCCATTATTAGCATTTATTATTGTC
  ATATTGCAAAGACCTTAATTAAAAGCGCACACAATCTTCCTGGAGAATACAATGAACATACCAAAAAACA
  GATGGAAACACGGAAACGCCTGGCTAAAATTGTGCTTGTCTTTGTGGGCTGTTTCATCTTCTGTTGGTTT
  CCAAACCACATCCTTTACATGTATCGGTCTTTCAACTATAATGAGATTGATCCATCTCTAGGCCACATGA
  TTGTCACCTTAGTTGCCCGGGTTCTCAGTTTTGGCAATTCTTGTGTCAACCCATTTGCTCTTTACCTACT
  CAGTGAAAGCTTCAGGAGGCATTTCAACAGCCAACTCTGCTGTGGGAGGAAGTCCTATCAAGAGAGAGGA
  ACCAGCTACCTACTCAGCTCTTCAGCGGTGCGTATGACATCTCTGAAAAGCAATGCTAAGAACATGGTGA
  CCAATTCTGTTTTACTAAATGGGCACAGCATGAAGCAGGAAATGGCAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213319 representing NM_002511
  Red=Cloning site Green=Tags(s)
  
  MPSKSLSNLSVTTGANESGSVPEGWERDFLPASDGTTTELVIRCVIPSLYLLIITVGLLGNIMLVKIFIT
  NSAMRSVPNIFISNLAAGDLLLLLTCVPVDASRYFFDEWMFGKVGCKLIPVIQLTSVGVSVFTLTALSAD
  RYRAIVNPMDMQTSGALLRTCVKAMGIWVVSVLLAVPEAVFSEVARISSLDNSSFTACIPYPQTDELHPK
  IHSVLIFLVYFLIPLAIISIYYCHIAKTLIKSAHNLPGEYNEHTKKQMETRKRLAKIVLVFVGCFIFCWF
  PNHILYMYRSFNYNEIDPSLGHMIVTLVARVLSFGNSCVNPFALYLLSESFRRHFNSQLCCGRKSYQERG
  TSYLLSSSAVRMTSLKSNAKNMVTNSVLLNGHSMKQEMAM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002511
• ORF Size: 1170 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002511.1, NM_002511.2, NM_002511.3, NP_002502.1
• RefSeq Size: 1352 bp
• RefSeq ORF: 1173 bp
• Locus ID: 4829
• Cytogenetics: 6q24.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 43.3 kDa
• Gene Summary: 'This gene encodes a 7-transmembrane G protein-coupled receptor that binds neuromedin B, which is a growth factor and mitogen for gastrointestinal epithelial tissue and for normal and neoplastic lung. This receptor may play a role in smooth muscle contraction, neuronal responses, and the regulation of cell growth. Antagonists of this receptor have a potential therapeutic use in inhibiting tumor cell growth. Polymorphisms in this gene may be associated with a susceptibility for schizophrenia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2016]'